PMID- 28237703
OWN - NLM
STAT- MEDLINE
DCOM- 20170613
LR  - 20171127
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 485
IP  - 2
DP  - 2017 Apr 1
TI  - Effect of cellular ubiquitin levels on the regulation of oxidative stress 
      response and proteasome function via Nrf1.
PG  - 234-240
LID - S0006-291X(17)30396-0 [pii]
LID - 10.1016/j.bbrc.2017.02.105 [doi]
AB  - The polyubiquitin genes Ubb and Ubc are upregulated under oxidative stress 
      induced by arsenite [As(III)]. However, the role of ubiquitin (Ub) under As(III) 
      exposure is not known in detail. In a previous study, we showed that the reduced 
      viability observed in Ubc(-/-) mouse embryonic fibroblasts under As(III) exposure 
      was not due to dysregulation of the Nrf2-Keap1 pathway, which prompted us to 
      investigate another NFE2 family protein, nuclear factor erythroid 2-related 
      factor 1 (Nrf1). In this study, we found that Ub deficiency due to Ubc knockdown 
      in N2a cells reduced cell viability and proteasome activity under As(III) 
      exposure. Furthermore, mRNA levels of the proteasome subunit Psma1 were also 
      reduced. In addition, Ub deficiency led to the nuclear accumulation of the p65 
      isoform of Nrf1 under As(III) exposure. Interestingly, the overexpression of 
      p65-Nrf1 recapitulated the phenotypes of Ub-deficient N2a cells under As(III) 
      exposure. On the other hand, Nrf1 knockdown suppressed the death of Ub-deficient 
      N2a cells upon exposure to As(III). Therefore, the levels of p65-Nrf1 may play an 
      important role in the maintenance of cell viability under oxidative stress 
      induced by As(III).
CI  - Copyright (c) 2017 Elsevier Inc. All rights reserved.
FAU - Lee, Donghee
AU  - Lee D
AD  - Department of Life Science, University of Seoul, Seoul 02504, Republic of Korea.
FAU - Ryu, Kwon-Yul
AU  - Ryu KY
AD  - Department of Life Science, University of Seoul, Seoul 02504, Republic of Korea. 
      Electronic address: kyryu@uos.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170222
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Arsenites)
RN  - 0 (Nrf1 protein, mouse)
RN  - 0 (Nuclear Respiratory Factor 1)
RN  - 0 (Ubiquitin)
RN  - 0 (Ubiquitin C)
RN  - EC 3.4.25.1 (Proteasome Endopeptidase Complex)
RN  - N5509X556J (arsenite)
SB  - IM
MH  - Animals
MH  - Arsenites/*toxicity
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - Gene Expression Regulation/drug effects
MH  - Gene Knockdown Techniques
MH  - Mice
MH  - NIH 3T3 Cells
MH  - Nuclear Respiratory Factor 1/genetics/*metabolism
MH  - Oxidative Stress/*drug effects
MH  - Proteasome Endopeptidase Complex/genetics/*metabolism
MH  - Ubiquitin/genetics/*metabolism
MH  - Ubiquitin C/genetics/metabolism
OTO - NOTNLM
OT  - Arsenite
OT  - Nrf1
OT  - Oxidative stress
OT  - Polyubiquitin gene
OT  - Proteasome
OT  - Ubiquitin
EDAT- 2017/02/27 06:00
MHDA- 2017/06/14 06:00
CRDT- 2017/02/27 06:00
PHST- 2017/02/15 00:00 [received]
PHST- 2017/02/20 00:00 [accepted]
PHST- 2017/02/27 06:00 [pubmed]
PHST- 2017/06/14 06:00 [medline]
PHST- 2017/02/27 06:00 [entrez]
AID - S0006-291X(17)30396-0 [pii]
AID - 10.1016/j.bbrc.2017.02.105 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2017 Apr 1;485(2):234-240. doi: 
      10.1016/j.bbrc.2017.02.105. Epub 2017 Feb 22.