PMID- 28239040
OWN - NLM
STAT- MEDLINE
DCOM- 20170918
LR  - 20181113
IS  - 1347-7439 (Electronic)
IS  - 0916-7250 (Print)
IS  - 0916-7250 (Linking)
VI  - 79
IP  - 4
DP  - 2017 Apr 8
TI  - Efficacy of Type 2 porcine reproductive and respiratory syndrome virus (PRRSV) 
      vaccine against the 2010 isolate of Vietnamese highly pathogenic PRRSV challenge 
      in pigs.
PG  - 765-773
LID - 10.1292/jvms.16-0536 [doi]
AB  - The efficacy of a commercial attenuated live type 2 porcine reproductive and 
      respiratory syndrome (PRRS) vaccine was tested under experimental infection with 
      a highly virulent Vietnamese virus isolated from a diseased pig affected with 
      highly pathogenic PRRS (HP-PRRS) using specific pathogen-free (SPF) pigs. 
      Twenty-five 4-week-old SPF pigs were divided into three groups as follows: pigs 
      vaccinated with a single dose of the vaccine (Group 1, n=10), unvaccinated pigs 
      (Group 2, n=10) and unvaccinated and non-infectious control pigs (Group 3, n=5). 
      Four weeks later, Groups 1 and 2 were challenged with a 1 ml inoculum containing 
      1 x 10(5.5) 50% tissue culture infectious dose (TCID(50))/ml of a Vietnamese 
      HP-PRRS virus isolated in 2010 via the intranasal route. Animals were monitored 
      during the subsequent two-week period post-challenge and necropsied for 
      virological and pathological assays. Results showed a significant reduction in 
      viral replication and shedding in vaccinated pigs compared to unvaccinated pigs. 
      The non-vaccinated pigs showed severe pyrogenic and respiratory illness with 
      marked systematic lesions including interstitial pneumonia and thymic atrophy. In 
      contrast, vaccinated pigs recovered quickly from fever with only mild 
      pathological manifestations. Therefore, although viral shedding was still noted, 
      immunization with the live PRRS vaccine did indeed reduce viral replication and 
      disease severity, suggesting its utility in minimizing outbreaks of HP-PRRS.
FAU - Iseki, Hiroshi
AU  - Iseki H
AD  - Viral Disease and Epidemiology Research Division, National Institute of Animal 
      Health, National Agriculture and Food Research Organization, 3-1-5 Kannondai, 
      Tsukuba, Ibaraki 305-0856 Japan.
FAU - Kawashima, Kenji
AU  - Kawashima K
FAU - Tung, Nguyen
AU  - Tung N
FAU - Inui, Kenjiro
AU  - Inui K
FAU - Ikezawa, Mitsutaka
AU  - Ikezawa M
FAU - Shibahara, Tomoyuki
AU  - Shibahara T
FAU - Yamakawa, Makoto
AU  - Yamakawa M
LA  - eng
PT  - Journal Article
PT  - Randomized Controlled Trial
DEP - 20170225
PL  - Japan
TA  - J Vet Med Sci
JT  - The Journal of veterinary medical science
JID - 9105360
RN  - 0 (Antibodies, Viral)
RN  - 0 (RNA, Viral)
RN  - 0 (Vaccines, Attenuated)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - Body Temperature
MH  - Porcine Reproductive and Respiratory Syndrome/immunology/pathology/*prevention & 
      control
MH  - *Porcine respiratory and reproductive syndrome virus
MH  - RNA, Viral/analysis
MH  - Swine
MH  - Vaccines, Attenuated/administration & dosage
MH  - Viral Load
MH  - Viral Vaccines/*administration & dosage
MH  - Virus Shedding
PMC - PMC5402200
EDAT- 2017/02/28 06:00
MHDA- 2017/09/19 06:00
PMCR- 2017/04/01
CRDT- 2017/02/28 06:00
PHST- 2017/02/28 06:00 [pubmed]
PHST- 2017/09/19 06:00 [medline]
PHST- 2017/02/28 06:00 [entrez]
PHST- 2017/04/01 00:00 [pmc-release]
AID - 16-0536 [pii]
AID - 10.1292/jvms.16-0536 [doi]
PST - ppublish
SO  - J Vet Med Sci. 2017 Apr 8;79(4):765-773. doi: 10.1292/jvms.16-0536. Epub 2017 Feb 
      25.