PMID- 28239394
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220311
IS  - 1678-9199 (Print)
IS  - 1678-9199 (Electronic)
IS  - 1678-9180 (Linking)
VI  - 23
DP  - 2017
TI  - Standardization and validation of Dot-ELISA assay for Paracoccidioides 
      brasiliensis antibody detection.
PG  - 11
LID - 10.1186/s40409-017-0101-3 [doi]
LID - 11
AB  - BACKGROUND: Paracoccidioidomycosis (PCM) is a neglected systemic mycosis caused 
      by a dimorphic fungus of the Paracoccidioides genus. The standard diagnosis is 
      based on isolation of the fungi in culture, and by microscopic visualization of 
      characteristic multiple budding yeast cells in biological samples. However, in 
      some situations, access to the site of injury prevents the collection of 
      biological material. A variety of immuno-serological techniques has proven useful 
      for allowing inferring diagnosis with a certain degree of certainty, thus 
      optimizing time. The aim of this study was to standardize and validate the 
      Dot-ELISA (DE) assay, comparing it with the serological standard, double 
      immunodiffusion (DI). METHODS: In order to standardize the DE assay, 143 serum 
      samples were used. Out of those, 23 were from apparently healthy patients, 77 
      were from patients with confirmed PCM and 43 were from patients with other lung 
      infections (tuberculosis, aspergillosis and histoplasmosis). To validate the DE 
      technique, 300 serum samples from patients with PCM clinical suspicion (probable 
      and possible cases) were employed, and these results were compared with those of 
      DI. RESULTS: The DE assay showed sensitivity of 91%, specificity of 95.4%, 
      positive predictive value of 96%, negative predictive value of 98.2%, accuracy of 
      93%, and great precision (k = 0.93). In addition, the nitrocellulose membranes 
      have proved to be viable for using at least 90 days after P. brasiliensis B-339 
      antigen sensitization. CONCLUSION: Dot-ELISA method was found to be an extremely 
      promising tool as serologic screening technique, because of its high sensitivity. 
      Furthermore, Dot-ELISA shows the prospect of being transferred to laboratories of 
      mycoserology including those with fewer resources or even to be used directly in 
      the field. It has an excellent shelf life - membranes coated with antigen can be 
      used for testing without changes in the pattern of reactivity among laboratories 
      - and presents reliable values of sensitivity, specificity, predictive values, 
      accuracy and a high correlation with the serological standard methodology. Based 
      on the present findings, it possible to state that this technique constitutes a 
      remarkable option to be used in routine diagnosis for public health centers.
FAU - Kamikawa, Camila Mika
AU  - Kamikawa CM
AD  - Laboratory of Mycosis Immunodiagnosis, Center of Immunology, Adolfo Lutz 
      Institute, Av. Dr. Arnaldo, 355, 11o andar, sala 1117 Sao Paulo, SP Brazil. ISNI: 
      0000 0004 0602 9808. GRID: grid.414596.b
AD  - Graduate Program in Sciences, Disease Control Coordination of the Sao Paulo State 
      Health Secretariat, Sao Paulo, SP Brazil.
FAU - Mendes, Rinaldo Poncio
AU  - Mendes RP
AD  - Department of Tropical Diseases, Botucatu Medical School, Sao Paulo State 
      University (UNESP - Univ Estadual Paulista), Botucatu, Sao Paulo State Brazil. 
      ISNI: 0000 0001 2188 478X. GRID: grid.410543.7
FAU - Vicentini, Adriana Pardini
AU  - Vicentini AP
AD  - Laboratory of Mycosis Immunodiagnosis, Center of Immunology, Adolfo Lutz 
      Institute, Av. Dr. Arnaldo, 355, 11o andar, sala 1117 Sao Paulo, SP Brazil. ISNI: 
      0000 0004 0602 9808. GRID: grid.414596.b
AD  - Graduate Program in Sciences, Disease Control Coordination of the Sao Paulo State 
      Health Secretariat, Sao Paulo, SP Brazil.
LA  - eng
PT  - Journal Article
DEP - 20170215
PL  - Brazil
TA  - J Venom Anim Toxins Incl Trop Dis
JT  - The journal of venomous animals and toxins including tropical diseases
JID - 101201501
PMC - PMC5312266
OTO - NOTNLM
OT  - Dot-ELISA
OT  - Immunodiagnostic tool
OT  - Paracoccidioides brasiliensis
OT  - Paracoccidioidomycosis
OT  - Validation
EDAT- 2017/02/28 06:00
MHDA- 2017/02/28 06:01
PMCR- 2017/02/15
CRDT- 2017/02/28 06:00
PHST- 2016/08/25 00:00 [received]
PHST- 2017/02/08 00:00 [accepted]
PHST- 2017/02/28 06:00 [entrez]
PHST- 2017/02/28 06:00 [pubmed]
PHST- 2017/02/28 06:01 [medline]
PHST- 2017/02/15 00:00 [pmc-release]
AID - 101 [pii]
AID - 10.1186/s40409-017-0101-3 [doi]
PST - epublish
SO  - J Venom Anim Toxins Incl Trop Dis. 2017 Feb 15;23:11. doi: 
      10.1186/s40409-017-0101-3. eCollection 2017.