PMID- 28241979 OWN - NLM STAT- MEDLINE DCOM- 20170821 LR - 20220408 IS - 1873-1244 (Electronic) IS - 0899-9007 (Linking) VI - 35 DP - 2017 Mar TI - Effects of omega-3 fatty acids on stereotypical behavior and social interactions in Wistar rats prenatally exposed to lipopolysaccarides. PG - 119-127 LID - S0899-9007(16)30255-6 [pii] LID - 10.1016/j.nut.2016.10.019 [doi] AB - OBJECTIVE: Supplementation with omega-3 polyunsaturated fatty acids (PUFAs) can positively contribute to neurologic development, modulating inflammatory responses, promoting homeostasis, and having a positive effect on animal behaviors associated with mental disorders. The aim of this study was to evaluate behavioral and biochemical effects of omega-3 fatty acid supplementation in an animal model for mental disorders by prenatal maternal exposure to lipopolysaccardies (LPS) from the maternal immune activation. METHODS: Twelve pregnant Wistar rats were used. Each rat received 100 mug/kg of LPS or saline solution on gestational day (GD) 9.5. The offspring remained with mothers until weaning and from postnatal day (PND) 30 were supplemented with omega-3 PUFA or saline solution by gavage at a dose of 0.8 g/kg orally for 21 d. On PND 52, the animals underwent behavioral tests; then, they were sacrificed, and the brain structures were dissected and analyzed by levels: neuron-specific enolase (NSE), brain-derived neurotrophic factor, and transforming growth factor (TGF)-beta. RESULT: Prenatal exposure to LPS significantly increased the episodes of stereotyped movements and decreased social interaction in the offspring (P = 0.009 and P = 0.001, respectively), after omega-3 PUFA supplementation these parameters reversed (P = 0.005 and P = 0.013, respectively). Significant changes also were identified in the biochemical analysis in NSE and TGF-beta in the brain structures; these conditions were reversed after omega-3 PUFA supplementation. CONCLUSION: Supplementation with omega-3 PUFA reversed animal behaviors that often are observed in autism and other mental disorders in rats prenatally exposed to LPS, and also exerted neuroprotective effects in marker levels of neuronal damage and expression of TGF-beta. CI - Copyright (c) 2016 Elsevier Inc. All rights reserved. FAU - Fortunato, Jucelia Jeremias AU - Fortunato JJ AD - Neurobiology Laboratory of Inflammatory and Metabolic Processes (NeuroIMet), Postgraduate Program in Health Sciences, University of Southern Santa Catarina, Tubarao, Santa Catarina, Brazil; Clinical Research Center, Nossa Senhora da Conceicao Hospital and the University of Southern Santa Catarina, Tubarao, Santa Catarina, Brazil. Electronic address: Jucelia.fortunato@unisul.br. FAU - da Rosa, Naiana AU - da Rosa N AD - Neurobiology Laboratory of Inflammatory and Metabolic Processes (NeuroIMet), Postgraduate Program in Health Sciences, University of Southern Santa Catarina, Tubarao, Santa Catarina, Brazil. FAU - Martins Laurentino, Ana Olivia AU - Martins Laurentino AO AD - Neurobiology Laboratory of Inflammatory and Metabolic Processes (NeuroIMet), Postgraduate Program in Health Sciences, University of Southern Santa Catarina, Tubarao, Santa Catarina, Brazil. FAU - Goulart, Marina AU - Goulart M AD - Neurobiology Laboratory of Inflammatory and Metabolic Processes (NeuroIMet), Postgraduate Program in Health Sciences, University of Southern Santa Catarina, Tubarao, Santa Catarina, Brazil. FAU - Michalak, Camila AU - Michalak C AD - Neurobiology Laboratory of Inflammatory and Metabolic Processes (NeuroIMet), Postgraduate Program in Health Sciences, University of Southern Santa Catarina, Tubarao, Santa Catarina, Brazil. FAU - Borges, Lidiane Pinto AU - Borges LP AD - Neurobiology Laboratory of Inflammatory and Metabolic Processes (NeuroIMet), Postgraduate Program in Health Sciences, University of Southern Santa Catarina, Tubarao, Santa Catarina, Brazil. FAU - da Cruz Cittadin Soares, Evandro AU - da Cruz Cittadin Soares E AD - Neurobiology Laboratory of Inflammatory and Metabolic Processes (NeuroIMet), Postgraduate Program in Health Sciences, University of Southern Santa Catarina, Tubarao, Santa Catarina, Brazil. FAU - Reis, Patricia Alves AU - Reis PA AD - Immunopharmacology Laboratory, Instituto Oswaldo Cruz/IOC/FIOCRUZ-Manguinhos, Rio de Janeiro, Brazil. FAU - de Castro Faria Neto, Hugo Caire AU - de Castro Faria Neto HC AD - Immunopharmacology Laboratory, Instituto Oswaldo Cruz/IOC/FIOCRUZ-Manguinhos, Rio de Janeiro, Brazil. FAU - Petronilho, Fabricia AU - Petronilho F AD - Neurobiology Laboratory of Inflammatory and Metabolic Processes (NeuroIMet), Postgraduate Program in Health Sciences, University of Southern Santa Catarina, Tubarao, Santa Catarina, Brazil. LA - eng PT - Journal Article DEP - 20161122 PL - United States TA - Nutrition JT - Nutrition (Burbank, Los Angeles County, Calif.) JID - 8802712 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Fatty Acids, Omega-3) RN - 0 (Lipopolysaccharides) RN - 0 (Transforming Growth Factor beta) SB - IM MH - Animals MH - Behavior, Animal/drug effects MH - Brain-Derived Neurotrophic Factor/genetics/metabolism MH - Dietary Supplements MH - Fatty Acids, Omega-3/*pharmacology MH - Female MH - *Interpersonal Relations MH - Lipopolysaccharides/*toxicity MH - Male MH - Pregnancy MH - *Prenatal Exposure Delayed Effects MH - Rats MH - Rats, Wistar MH - Stereotyped Behavior/*drug effects MH - Transforming Growth Factor beta/genetics/metabolism MH - Weaning OTO - NOTNLM OT - Lipopolysaccharides OT - Maternal immune activation OT - Neuron-specific enolase OT - Transforming growth factor-beta OT - omega-3 EDAT- 2017/03/01 06:00 MHDA- 2017/08/22 06:00 CRDT- 2017/03/01 06:00 PHST- 2016/05/24 00:00 [received] PHST- 2016/10/21 00:00 [revised] PHST- 2016/10/29 00:00 [accepted] PHST- 2017/03/01 06:00 [entrez] PHST- 2017/03/01 06:00 [pubmed] PHST- 2017/08/22 06:00 [medline] AID - S0899-9007(16)30255-6 [pii] AID - 10.1016/j.nut.2016.10.019 [doi] PST - ppublish SO - Nutrition. 2017 Mar;35:119-127. doi: 10.1016/j.nut.2016.10.019. Epub 2016 Nov 22.