PMID- 28243358
OWN - NLM
STAT- MEDLINE
DCOM- 20170316
LR  - 20181113
IS  - 1942-0994 (Electronic)
IS  - 1942-0900 (Print)
IS  - 1942-0994 (Linking)
VI  - 2017
DP  - 2017
TI  - Hydrogen-Rich Water Ameliorates Total Body Irradiation-Induced Hematopoietic Stem 
      Cell Injury by Reducing Hydroxyl Radical.
PG  - 8241678
LID - 10.1155/2017/8241678 [doi]
LID - 8241678
AB  - We examined whether consumption of hydrogen-rich water (HW) could ameliorate 
      hematopoietic stem cell (HSC) injury in mice with total body irradiation (TBI). 
      The results indicated that HW alleviated TBI-induced HSC injury with respect to 
      cell number alteration and to the self-renewal and differentiation of HSCs. HW 
      specifically decreased hydroxyl radical ((∙)OH) levels in the c-kit(+) cells of 
      4 Gy irradiated mice. Proliferative bone marrow cells (BMCs) increased and 
      apoptotic c-kit(+) cells decreased in irradiated mice uptaken with HW. In 
      addition, the mean fluorescence intensity (MFI) of gamma-H2AX and percentage of 
      8-oxoguanine positive cells significantly decreased in HW-treated c-kit(+) cells, 
      indicating that HW can alleviate TBI-induced DNA damage and oxidative DNA damage 
      in c-kit(+) cells. Finally, the cell cycle (P21), cell apoptosis (BCL-XL and 
      BAK), and oxidative stress (NRF2, HO-1, NQO1, SOD, and GPX1) proteins were 
      significantly altered by HW in irradiated mouse c-kit(+) cells. Collectively, the 
      present results suggest that HW protects against TBI-induced HSC injury.
FAU - Zhang, Junling
AU  - Zhang J
AUID- ORCID: 0000-0003-4321-3156
AD  - Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, 
      Institute of Radiation Medicine, Peking Union Medical College and Chinese Academy 
      of Medical Science, Tianjin 300192, China.
FAU - Xue, Xiaolei
AU  - Xue X
AD  - Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, 
      Institute of Radiation Medicine, Peking Union Medical College and Chinese Academy 
      of Medical Science, Tianjin 300192, China.
FAU - Han, Xiaodan
AU  - Han X
AD  - Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, 
      Institute of Radiation Medicine, Peking Union Medical College and Chinese Academy 
      of Medical Science, Tianjin 300192, China.
FAU - Li, Yuan
AU  - Li Y
AD  - Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, 
      Institute of Radiation Medicine, Peking Union Medical College and Chinese Academy 
      of Medical Science, Tianjin 300192, China.
FAU - Lu, Lu
AU  - Lu L
AD  - Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, 
      Institute of Radiation Medicine, Peking Union Medical College and Chinese Academy 
      of Medical Science, Tianjin 300192, China.
FAU - Li, Deguan
AU  - Li D
AD  - Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, 
      Institute of Radiation Medicine, Peking Union Medical College and Chinese Academy 
      of Medical Science, Tianjin 300192, China.
FAU - Fan, Saijun
AU  - Fan S
AUID- ORCID: 0000-0003-0691-6864
AD  - Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, 
      Institute of Radiation Medicine, Peking Union Medical College and Chinese Academy 
      of Medical Science, Tianjin 300192, China.
LA  - eng
PT  - Journal Article
DEP - 20170122
PL  - United States
TA  - Oxid Med Cell Longev
JT  - Oxidative medicine and cellular longevity
JID - 101479826
RN  - 0 (Protective Agents)
RN  - 059QF0KO0R (Water)
RN  - 3352-57-6 (Hydroxyl Radical)
RN  - 7YNJ3PO35Z (Hydrogen)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - DNA Damage/drug effects/radiation effects
MH  - Flow Cytometry
MH  - Hematopoietic Stem Cell Transplantation
MH  - Hematopoietic Stem Cells/*drug effects/radiation effects
MH  - Hydrogen/*pharmacology
MH  - Hydroxyl Radical/*metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Oxidative Stress/*drug effects/radiation effects
MH  - Protective Agents/*pharmacology
MH  - Radiation Injuries, Experimental/prevention & control
MH  - Water/*chemistry
MH  - *Whole-Body Irradiation
PMC - PMC5294227
COIS- The authors declare that they have no competing interests.
EDAT- 2017/03/01 06:00
MHDA- 2017/03/17 06:00
PMCR- 2017/01/22
CRDT- 2017/03/01 06:00
PHST- 2016/08/04 00:00 [received]
PHST- 2016/11/08 00:00 [revised]
PHST- 2016/12/26 00:00 [accepted]
PHST- 2017/03/01 06:00 [entrez]
PHST- 2017/03/01 06:00 [pubmed]
PHST- 2017/03/17 06:00 [medline]
PHST- 2017/01/22 00:00 [pmc-release]
AID - 10.1155/2017/8241678 [doi]
PST - ppublish
SO  - Oxid Med Cell Longev. 2017;2017:8241678. doi: 10.1155/2017/8241678. Epub 2017 Jan 
      22.