PMID- 28244988
OWN - NLM
STAT- MEDLINE
DCOM- 20170425
LR  - 20220318
IS  - 1552-4469 (Electronic)
IS  - 1552-4450 (Print)
IS  - 1552-4450 (Linking)
VI  - 13
IP  - 5
DP  - 2017 May
TI  - The Arabidopsis O-fucosyltransferase SPINDLY activates nuclear growth repressor 
      DELLA.
PG  - 479-485
LID - 10.1038/nchembio.2320 [doi]
AB  - Plant development requires coordination among complex signaling networks to 
      enhance the plant's adaptation to changing environments. DELLAs, transcription 
      regulators originally identified as repressors of phytohormone gibberellin 
      signaling, play a central role in integrating multiple signaling activities via 
      direct protein interactions with key transcription factors. Here, we found that 
      DELLA is mono-O-fucosylated by the novel O-fucosyltransferase SPINDLY (SPY) in 
      Arabidopsis thaliana. O-fucosylation activates DELLA by promoting its interaction 
      with key regulators in brassinosteroid- and light-signaling pathways, including 
      BRASSINAZOLE-RESISTANT1 (BZR1), PHYTOCHROME-INTERACTING-FACTOR3 (PIF3) and PIF4. 
      Moreover, spy mutants displayed elevated responses to gibberellin and 
      brassinosteroid, and increased expression of common target genes of DELLAs, BZR1 
      and PIFs. Our study revealed that SPY-dependent protein O-fucosylation plays a 
      key role in regulating plant development. This finding may have broader 
      importance because SPY orthologs are conserved in prokaryotes and eukaryotes, 
      thus suggesting that intracellular O-fucosylation may regulate a wide range of 
      biological processes in diverse organisms.
FAU - Zentella, Rodolfo
AU  - Zentella R
AD  - Department of Biology, Duke University, Durham, North Carolina, USA.
FAU - Sui, Ning
AU  - Sui N
AD  - Department of Biology, Duke University, Durham, North Carolina, USA.
FAU - Barnhill, Benjamin
AU  - Barnhill B
AD  - Department of Chemistry, University of Virginia, Charlottesville, Virginia, USA.
FAU - Hsieh, Wen-Ping
AU  - Hsieh WP
AD  - Department of Biology, Duke University, Durham, North Carolina, USA.
FAU - Hu, Jianhong
AU  - Hu J
AD  - Department of Biology, Duke University, Durham, North Carolina, USA.
FAU - Shabanowitz, Jeffrey
AU  - Shabanowitz J
AD  - Department of Chemistry, University of Virginia, Charlottesville, Virginia, USA.
FAU - Boyce, Michael
AU  - Boyce M
AD  - Department of Biochemistry, Duke University Medical Center, Durham, North 
      Carolina, USA.
FAU - Olszewski, Neil E
AU  - Olszewski NE
AUID- ORCID: 0000-0001-5393-7743
AD  - Department of Plant Biology, University of Minnesota, Saint Paul, Minnesota, USA.
FAU - Zhou, Pei
AU  - Zhou P
AD  - Department of Biochemistry, Duke University Medical Center, Durham, North 
      Carolina, USA.
AD  - Department of Chemistry, Duke University, Durham, North Carolina, USA.
FAU - Hunt, Donald F
AU  - Hunt DF
AD  - Department of Chemistry, University of Virginia, Charlottesville, Virginia, USA.
AD  - Department of Pathology, University of Virginia, Charlottesville, Virginia, USA.
FAU - Sun, Tai-Ping
AU  - Sun TP
AD  - Department of Biology, Duke University, Durham, North Carolina, USA.
LA  - eng
GR  - R01 GM037537/GM/NIGMS NIH HHS/United States
GR  - R01 GM100051/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20170228
PL  - United States
TA  - Nat Chem Biol
JT  - Nature chemical biology
JID - 101231976
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (GAI protein, Arabidopsis)
RN  - 0 (Repressor Proteins)
RN  - 0 (SPY protein, Arabidopsis)
RN  - EC 2.4.1.- (Fucosyltransferases)
SB  - IM
MH  - Arabidopsis/enzymology/genetics/*metabolism
MH  - Arabidopsis Proteins/genetics/*metabolism
MH  - Fucosyltransferases/genetics/*metabolism
MH  - Repressor Proteins/genetics/*metabolism
PMC - PMC5391292
MID - NIHMS833882
COIS- Competing financial interests statements The authors declare no competing 
      financial interests.
EDAT- 2017/03/01 06:00
MHDA- 2017/04/26 06:00
PMCR- 2017/08/28
CRDT- 2017/03/01 06:00
PHST- 2016/08/04 00:00 [received]
PHST- 2016/12/01 00:00 [accepted]
PHST- 2017/03/01 06:00 [pubmed]
PHST- 2017/04/26 06:00 [medline]
PHST- 2017/03/01 06:00 [entrez]
PHST- 2017/08/28 00:00 [pmc-release]
AID - nchembio.2320 [pii]
AID - 10.1038/nchembio.2320 [doi]
PST - ppublish
SO  - Nat Chem Biol. 2017 May;13(5):479-485. doi: 10.1038/nchembio.2320. Epub 2017 Feb 
      28.