PMID- 28245527
OWN - NLM
STAT- MEDLINE
DCOM- 20180430
LR  - 20181202
IS  - 1525-1594 (Electronic)
IS  - 0160-564X (Linking)
VI  - 41
IP  - 7
DP  - 2017 Jul
TI  - Isolation of Mesenchymal Stromal Cells From Peripheral Blood of ST Elevation 
      Myocardial Infarction Patients.
PG  - 654-666
LID - 10.1111/aor.12829 [doi]
AB  - Bone marrow mesenchymal stromal cells (MSCs) have shown therapeutic potential in 
      the treatment of myocardial infarction patients. However, bone marrow requires 
      invasive harvesting techniques. Therefore, the aim was to carry out a feasibility 
      study of using autologous peripheral blood (PB) as a source for MSCs and platelet 
      lysate (PL), a potential novel therapeutic intervention in acute ST elevation 
      myocardial infarction (STEMI) patients. Autologous PL and MSCs were prepared from 
      STEMI patient and healthy control blood. MSCs were analyzed by trilineage 
      differentiation and flow cytometry. PB MSCs were isolated from 83% of patients 
      (n = 6) but not from controls. The use of PL was feasible in the first passage 
      but not in subsequent ones due to volume. To conclude, PB is a promising 
      alternative to bone marrow. It negates the need for invasive harvesting 
      techniques, and reduces hemorrhagic risk in this patient population routinely 
      managed with anticoagulant and antiplatelet agents.
CI  - (c) 2017 International Center for Artificial Organs and Transplantation and Wiley 
      Periodicals, Inc.
FAU - Pieper, Ina Laura
AU  - Pieper IL
AD  - Swansea University Medical School, Institute of Life Science.
FAU - Smith, Rachel
AU  - Smith R
AD  - Swansea University Medical School, Institute of Life Science.
FAU - Bishop, Joanna C
AU  - Bishop JC
AD  - Swansea University Medical School, Institute of Life Science.
FAU - Aldalati, Omar
AU  - Aldalati O
AD  - Regional Cardiac Centre, Morriston Hospital, Swansea, Wales, UK.
FAU - Chase, Alex J
AU  - Chase AJ
AD  - Regional Cardiac Centre, Morriston Hospital, Swansea, Wales, UK.
FAU - Morgan, Gareth
AU  - Morgan G
AD  - Swansea University Medical School, Institute of Life Science.
FAU - Thornton, Catherine A
AU  - Thornton CA
AD  - Swansea University Medical School, Institute of Life Science.
LA  - eng
PT  - Journal Article
DEP - 20170228
PL  - United States
TA  - Artif Organs
JT  - Artificial organs
JID - 7802778
RN  - 0 (Antigens, CD)
SB  - IM
MH  - Aged
MH  - Antigens, CD/analysis
MH  - Blood Cell Count
MH  - Cell Differentiation
MH  - Cell Separation/*methods
MH  - Feasibility Studies
MH  - Female
MH  - Humans
MH  - Male
MH  - Mesenchymal Stem Cells/*cytology/pathology
MH  - Middle Aged
MH  - ST Elevation Myocardial Infarction/*blood/pathology
OTO - NOTNLM
OT  - Mesenchymal stromal cells-Cardiology-Cell therapy-Regenerative medicine
EDAT- 2017/03/01 06:00
MHDA- 2018/05/01 06:00
CRDT- 2017/03/01 06:00
PHST- 2016/04/30 00:00 [received]
PHST- 2016/06/16 00:00 [revised]
PHST- 2016/06/30 00:00 [accepted]
PHST- 2017/03/01 06:00 [pubmed]
PHST- 2018/05/01 06:00 [medline]
PHST- 2017/03/01 06:00 [entrez]
AID - 10.1111/aor.12829 [doi]
PST - ppublish
SO  - Artif Organs. 2017 Jul;41(7):654-666. doi: 10.1111/aor.12829. Epub 2017 Feb 28.