PMID- 28245636
OWN - NLM
STAT- MEDLINE
DCOM- 20170420
LR  - 20181113
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 18
IP  - 3
DP  - 2017 Feb 26
TI  - A Comparison of the Effects of Benzalkonium Chloride on Ocular Surfaces between 
      C57BL/6 and BALB/c Mice.
LID - 10.3390/ijms18030509 [doi]
LID - 509
AB  - Models of benzalkonium chloride (BAC)-induced ocular disruption have been created 
      and are widely used in various animals. This study aimed to compare the effects 
      of BAC on the ocular surfaces of C57BL/6 and BALB/c mice. C57BL/6 and BALB/c mice 
      were treated separately with BAC eye-drops at different concentrations. Eyes were 
      evaluated by scoring epithelial disruption, corneal opacity and 
      neovascularization in vivo, and by histological assays with hematoxylin/eosin 
      (H/E) and periodic acid-Schiff stainings and by determining the expression of 
      inflammatory factors in vitro on Days 7 and 14. The in vivo corneal epithelial 
      disruption, corneal edema/opacity and neovascularization, which were in 
      accordance with the results of the H/E staining and peaked at Day 7, were 
      observed in a dose-dependent manner in the BAC-treated mice, with more severe 
      signs in the C57BL/6 mice than the BALB/c mice. The loss of conjunctival goblet 
      cells in the conjunctivas and the increasing expression of monocyte 
      chemoattractant protein 1 (MCP-1), growth-regulated protein alpha (GROa) and 
      macrophage inflammatory protein-1 alpha (MIP-1a) in the corneas were found in a 
      dose-dependent manner in both strains of mice. Topical application of BAC can 
      dramatically disrupt the ocular surfaces of C57BL/6 and BALB/c mice, and the 
      disruptions were much more severe in the C57BL/6 mice that received high doses of 
      BAC.
FAU - Yang, Qian
AU  - Yang Q
AD  - Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China. 
      muyangmay@126.com.
FAU - Zhang, Yafang
AU  - Zhang Y
AD  - Department of Ophthalmology, Hubei University of Science and Technology, Xianning 
      437100, China. zhangyafang709@163.com.
FAU - Liu, Xiuping
AU  - Liu X
AD  - Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China. 
      LXP__2171@126.com.
FAU - Wang, Nan
AU  - Wang N
AD  - Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China. 
      yankewangnan@126.com.
FAU - Song, Zhenyu
AU  - Song Z
AD  - Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China. 
      songzhenyu08@sina.com.
FAU - Wu, Kaili
AU  - Wu K
AD  - Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China. 
      wukaili@mail.sysu.edu.cn.
AD  - Department of Ophthalmology, Hubei University of Science and Technology, Xianning 
      437100, China. wukaili@mail.sysu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20170226
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Anti-Infective Agents, Local)
RN  - 0 (Benzalkonium Compounds)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Ophthalmic Solutions)
SB  - IM
MH  - Animals
MH  - Anti-Infective Agents, Local/administration & dosage/*pharmacology
MH  - Benzalkonium Compounds/administration & dosage/*pharmacology
MH  - Conjunctiva/drug effects/metabolism/pathology
MH  - Cornea/drug effects/metabolism/pathology
MH  - Disease Models, Animal
MH  - Female
MH  - Inflammation Mediators/metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Ophthalmic Solutions/administration & dosage/*pharmacology
MH  - Xerophthalmia/drug therapy/metabolism/pathology
PMC - PMC5372525
OTO - NOTNLM
OT  - BALB/c
OT  - C57BL/6
OT  - benzalkonium chloride
OT  - conjunctiva
OT  - cornea
OT  - mouse
COIS- The authors declare no conflict of interest.
EDAT- 2017/03/02 06:00
MHDA- 2017/04/21 06:00
PMCR- 2017/03/01
CRDT- 2017/03/02 06:00
PHST- 2017/01/01 00:00 [received]
PHST- 2017/02/13 00:00 [revised]
PHST- 2017/02/23 00:00 [accepted]
PHST- 2017/03/02 06:00 [entrez]
PHST- 2017/03/02 06:00 [pubmed]
PHST- 2017/04/21 06:00 [medline]
PHST- 2017/03/01 00:00 [pmc-release]
AID - ijms18030509 [pii]
AID - ijms-18-00509 [pii]
AID - 10.3390/ijms18030509 [doi]
PST - epublish
SO  - Int J Mol Sci. 2017 Feb 26;18(3):509. doi: 10.3390/ijms18030509.