PMID- 28246355
OWN - NLM
STAT- MEDLINE
DCOM- 20180129
LR  - 20181113
IS  - 1573-4935 (Electronic)
IS  - 0144-8463 (Print)
IS  - 0144-8463 (Linking)
VI  - 37
IP  - 2
DP  - 2017 Apr 28
TI  - Association of A1538G and C2437T single nucleotide polymorphisms in heat shock 
      protein-70 genes with diabetic nephropathy among South Indian population.
LID - BSR20160605 [pii]
LID - 10.1042/BSR20160605 [doi]
AB  - Diabetic Nephropathy (DN) is the leading cause of end-stage renal disease, 
      characterized by progressive albuminuria and conferring additional risk of 
      cardiovascular disease (CVD) and mortality. The crucial role of heat-shock 
      proteins (HSPs) on renal function in patients with DN has been well documented. 
      The present study was aimed to understand the association of HSP-70 gene variants 
      on the susceptibility of Type 2 Diabetes Mellitus (T2DM) and DN. A total of 946 
      subjects (549 Males; 397 Females) were recruited and divided into four groups 
      according to the levels of urinary albumin excretion (UAE): those with 
      normoalbuminuria (UAE <30 mg/24 h; n=230), those with microalbuminuria (30</= UAE 
      </=300 mg/24 h; n=230), and those with macroalbuminuria (UAE> 300 mg/24 h; n=230). 
      The control group randomly enrolled a consecutive population of 256 healthy 
      subjects who had a routine medical check-up in our hospital. Those subjects had 
      no history or clinical symptoms of diabetes. Subjects were genotyped for HSP70-2 
      (+1538 A/G; rs2763979) and HSP70-hom (+2437 C/T; rs2227956) by PCR-restriction 
      fragment length polymorphism (RFLP). The 'G' allele of HSP70-2 (+1538 A/G) single 
      nucleotide polymorphism (SNP) showed relative risk for normoalbuminuria, 
      microalbuminuria and macroalbuminuria subjects whereas the 'T' allele of 
      HSP70-hom (+2437 C/T) SNP showed significant protection against macroalbuminuria 
      subjects. In conclusion, our results indicate that the HSP70-2 (+1538 A/G) and 
      HSP70-hom (+2437 C/T) SNPs are highly associated with renal complications in T2DM 
      among the South Indian population.
CI  - (c) 2017 The Author(s).
FAU - Dhamodharan, Umapathy
AU  - Dhamodharan U
AD  - Life Science Division, SRM Research Institute, SRM University, Kattankulathur, 
      Chennai-603203, India.
FAU - Ezhilarasi, Krishnamoorthy
AU  - Ezhilarasi K
AD  - Department of Biochemistry and Molecular Genetics, Prof. M. Viswanathan Diabetes 
      Research Centre and M.V. Hospital for Diabetes (A WHO Collaborating Centre for 
      Research, Education & Training in Diabetes), Royapuram, Chennai-600013, India.
FAU - Ponjayanthi, Balashanmugam
AU  - Ponjayanthi B
AD  - Life Science Division, SRM Research Institute, SRM University, Kattankulathur, 
      Chennai-603203, India.
FAU - Sireesh, Dornadula
AU  - Sireesh D
AD  - Life Science Division, SRM Research Institute, SRM University, Kattankulathur, 
      Chennai-603203, India.
FAU - Ramkumar, Kunka Mohanram
AU  - Ramkumar KM
AD  - Life Science Division, SRM Research Institute, SRM University, Kattankulathur, 
      Chennai-603203, India drvijay@mvdiabetes.com ramkumar.km@res.srmuniv.ac.in.
FAU - Viswanathan, Vijay
AU  - Viswanathan V
AD  - Department of Biochemistry and Molecular Genetics, Prof. M. Viswanathan Diabetes 
      Research Centre and M.V. Hospital for Diabetes (A WHO Collaborating Centre for 
      Research, Education & Training in Diabetes), Royapuram, Chennai-600013, India 
      drvijay@mvdiabetes.com ramkumar.km@res.srmuniv.ac.in.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170327
PL  - England
TA  - Biosci Rep
JT  - Bioscience reports
JID - 8102797
RN  - 0 (Biomarkers)
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 0 (Mitochondrial Proteins)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Albuminuria/urine
MH  - Biomarkers/blood/urine
MH  - Diabetes Mellitus, Type 2/*complications/epidemiology
MH  - Diabetic Nephropathies/epidemiology/*genetics
MH  - Female
MH  - *Genetic Predisposition to Disease
MH  - Genotype
MH  - HSP70 Heat-Shock Proteins/blood/*genetics
MH  - Humans
MH  - India/epidemiology
MH  - Male
MH  - Middle Aged
MH  - Mitochondrial Proteins/blood/*genetics
MH  - Pilot Projects
MH  - Polymorphism, Single Nucleotide
MH  - Risk Factors
MH  - Statistics, Nonparametric
MH  - Time Factors
PMC - PMC5469327
OTO - NOTNLM
OT  - Diabetic Nephropathy
OT  - Heat-shock proteins
OT  - Macroalbuminuria
OT  - Microalbuminuria
OT  - Single Nucleotide Polymorphism
OT  - Type 2 Diabetes Mellitus
COIS- The authors declare that there are no competing interests associated with the 
      manuscript.
EDAT- 2017/03/02 06:00
MHDA- 2018/01/30 06:00
PMCR- 2017/03/27
CRDT- 2017/03/02 06:00
PHST- 2016/12/21 00:00 [received]
PHST- 2017/02/20 00:00 [revised]
PHST- 2017/02/27 00:00 [accepted]
PHST- 2017/03/02 06:00 [pubmed]
PHST- 2018/01/30 06:00 [medline]
PHST- 2017/03/02 06:00 [entrez]
PHST- 2017/03/27 00:00 [pmc-release]
AID - BSR20160605 [pii]
AID - 10.1042/BSR20160605 [doi]
PST - epublish
SO  - Biosci Rep. 2017 Mar 27;37(2):BSR20160605. doi: 10.1042/BSR20160605. Print 2017 
      Apr 28.