PMID- 28248341
OWN - NLM
STAT- MEDLINE
DCOM- 20180205
LR  - 20181023
IS  - 1539-4794 (Electronic)
IS  - 0146-9592 (Linking)
VI  - 42
IP  - 5
DP  - 2017 Mar 1
TI  - Functional optoacoustic neuro-tomography of calcium fluxes in adult zebrafish 
      brain in vivo.
PG  - 959-962
LID - 10.1364/OL.42.000959 [doi]
AB  - Genetically-encoded calcium indicators (GECIs) have revolutionized neuroimaging 
      by enabling mapping of the activity of entire neuronal populations in vivo. 
      Visualization of these powerful activity sensors has to date been limited to 
      depth-restricted microscopic studies due to intense light scattering in the 
      brain. We demonstrate, for the first time, in vivo real-time volumetric 
      optoacoustic monitoring of calcium transients in adult transgenic zebrafish 
      expressing the GCaMP5G calcium indicator. Fast changes in optoacoustic traces 
      associated with GCaMP5G activity were detectable in the presence of other 
      strongly absorbing endogenous chromophores, such as hemoglobin. The new 
      functional optoacoustic neuroimaging method can visualize neural activity at 
      penetration depths and spatio-temporal resolution scales not covered with the 
      existing neuroimaging techniques.
FAU - Dean-Ben, X Luis
AU  - Dean-Ben XL
FAU - Gottschalk, Sven
AU  - Gottschalk S
FAU - Sela, Gali
AU  - Sela G
FAU - Shoham, Shy
AU  - Shoham S
FAU - Razansky, Daniel
AU  - Razansky D
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Opt Lett
JT  - Optics letters
JID - 7708433
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Animals, Genetically Modified
MH  - Brain/*metabolism
MH  - Calcium/*metabolism
MH  - Neurons
MH  - *Photoacoustic Techniques
MH  - Tomography
MH  - Tomography, X-Ray Computed/*methods
MH  - Zebrafish
EDAT- 2017/03/02 06:00
MHDA- 2018/02/06 06:00
CRDT- 2017/03/02 06:00
PHST- 2017/03/02 06:00 [entrez]
PHST- 2017/03/02 06:00 [pubmed]
PHST- 2018/02/06 06:00 [medline]
AID - 360365 [pii]
AID - 10.1364/OL.42.000959 [doi]
PST - ppublish
SO  - Opt Lett. 2017 Mar 1;42(5):959-962. doi: 10.1364/OL.42.000959.