PMID- 28249227
OWN - NLM
STAT- MEDLINE
DCOM- 20170606
LR  - 20181202
IS  - 1879-1514 (Electronic)
IS  - 0166-445X (Linking)
VI  - 186
DP  - 2017 May
TI  - Effects of food-borne exposure of juvenile rainbow trout (Oncorhynchus mykiss) to 
      emerging brominated flame retardants 1,2-bis(2,4,6-tribromophenoxy)ethane and 
      2-ethylhexyl-2,3,4,5-tetrabromobenzoate.
PG  - 40-49
LID - S0166-445X(17)30054-1 [pii]
LID - 10.1016/j.aquatox.2017.02.023 [doi]
AB  - Brominated flame retardants (BFRs) represent a large group of chemicals used in a 
      variety of household and commercial products to prevent fire propagation. The 
      environmental persistence and toxicity of some of the most widely used BFRs has 
      resulted in a progressive ban worldwide and the development of novel BFRs for 
      which the knowledge on environmental health impacts remains limited. The 
      objectives of this study were to evaluate the effects of two emerging BFRs, 
      1,2-bis(2,4,6-tribromophenoxy)ethane (BTBPE) and 
      2-ethylhexyl-2,3,4,5-tetrabromobenzoate (EH-TBB), in diet exposed juvenile 
      rainbow trout (Oncorhynchus mykiss). Both compounds were detected in fish 
      carcasses at 76% and 2% of the daily dosage of BTBPE and EH-TBB, respectively, 
      indicating accumulation of BTBPE and by contrast extensive depuration/metabolism 
      of EH-TBB. Liver gene transcription analysis using RNA-sequencing indicated that 
      the chronic 28-d dietary exposure of trout to EH-TBB down-regulated one single 
      gene related to endocrine-mediated processes, whereas BTBPE impacted the 
      transcription of 33 genes, including genes involved in the immune response, 
      reproduction, and oxidative stress. Additional analysis using qRT-PCR after 48-h 
      and 28-d of exposure confirmed the impact of BTBPE on immune related genes in the 
      liver (apolipoprotein A-I, lysozyme) and the head-kidney (complement c3-4). 
      However, the activity of lysozymes measured at the protein level did not reflect 
      transcriptomic results. Overall, results suggested an impact on immune-related 
      gene transcription in BTBPE exposed fish, as well as oxidative stress and 
      endocrine disruption potentials.
CI  - Crown Copyright (c) 2017. Published by Elsevier B.V. All rights reserved.
FAU - Giraudo, Maeva
AU  - Giraudo M
AD  - Environment and Climate Change Canada, Aquatic Contaminants Research Division, 
      105 McGill Street, Montreal, QC, H2Y 2E7 Canada. Electronic address: 
      maeva.giraudo@gmail.com.
FAU - Douville, Melanie
AU  - Douville M
AD  - Environment and Climate Change Canada, Aquatic Contaminants Research Division, 
      105 McGill Street, Montreal, QC, H2Y 2E7 Canada.
FAU - Letcher, Robert J
AU  - Letcher RJ
AD  - Environment and Climate Change Canada, Ecotoxicology and Wildlife Health 
      Division, National Wildlife Research Centre, Bldg. 33, 1125 Colonel By Dr. (Raven 
      Road), Carleton University, Ottawa, ON, K1A 0H3 Canada.
FAU - Houde, Magali
AU  - Houde M
AD  - Environment and Climate Change Canada, Aquatic Contaminants Research Division, 
      105 McGill Street, Montreal, QC, H2Y 2E7 Canada.
LA  - eng
PT  - Journal Article
DEP - 20170222
PL  - Netherlands
TA  - Aquat Toxicol
JT  - Aquatic toxicology (Amsterdam, Netherlands)
JID - 8500246
RN  - 0 (2-ethylhexyl 2,3,4,5-tetrabromobenzoate)
RN  - 0 (Benzoates)
RN  - 0 (Bromobenzenes)
RN  - 0 (Flame Retardants)
RN  - 0 (RNA, Messenger)
RN  - 0 (Water Pollutants, Chemical)
RN  - 34I00D6RNM (1,2-bis(2,4,6-tribromophenoxy)ethane)
RN  - EC 3.2.1.17 (Muramidase)
SB  - IM
MH  - Animals
MH  - Benzoates/chemistry/*toxicity
MH  - Bromobenzenes/chemistry/*toxicity
MH  - Diet
MH  - Environmental Exposure/*analysis
MH  - Flame Retardants/*toxicity
MH  - *Food
MH  - Head Kidney/drug effects/metabolism
MH  - Liver/drug effects/metabolism
MH  - Molecular Sequence Annotation
MH  - Molecular Weight
MH  - Muramidase/metabolism
MH  - Oncorhynchus mykiss/growth & development/*metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Real-Time Polymerase Chain Reaction
MH  - Sequence Analysis, RNA
MH  - Transcription, Genetic/drug effects
MH  - Transcriptome/drug effects/genetics
MH  - Water Pollutants, Chemical/chemistry/toxicity
OTO - NOTNLM
OT  - BTBPE
OT  - EHTBB
OT  - Flame retardants
OT  - Gene transcription
OT  - RNA-sequencing
OT  - Rainbow trout
EDAT- 2017/03/02 06:00
MHDA- 2017/06/07 06:00
CRDT- 2017/03/02 06:00
PHST- 2017/01/04 00:00 [received]
PHST- 2017/02/20 00:00 [revised]
PHST- 2017/02/21 00:00 [accepted]
PHST- 2017/03/02 06:00 [pubmed]
PHST- 2017/06/07 06:00 [medline]
PHST- 2017/03/02 06:00 [entrez]
AID - S0166-445X(17)30054-1 [pii]
AID - 10.1016/j.aquatox.2017.02.023 [doi]
PST - ppublish
SO  - Aquat Toxicol. 2017 May;186:40-49. doi: 10.1016/j.aquatox.2017.02.023. Epub 2017 
      Feb 22.