PMID- 28249643
OWN - NLM
STAT- MEDLINE
DCOM- 20180326
LR  - 20180530
IS  - 1879-0593 (Electronic)
IS  - 1368-8375 (Linking)
VI  - 66
DP  - 2017 Mar
TI  - Chromosome instability in tumor resection margins of primary OSCC is a predictor 
      of local recurrence.
PG  - 14-21
LID - S1368-8375(16)30276-7 [pii]
LID - 10.1016/j.oraloncology.2016.12.029 [doi]
AB  - BACKGROUND: The local recurrence rate in oral squamous cell cancer (OSCC) hardly 
      decreases. This is partly due to the presence of (pre)malignant cells in the 
      remaining tissue after resection, that may lead to the development of a new tumor 
      in time. Detection of histologically (pre)malignant cells in the tumor resection 
      margins should predict these patients at risk for recurrence, however this 
      appears to be difficult in routine practice. Purpose of this study was to apply 
      easy-to-use molecular tests for more accurate detection of (pre)malignant cells 
      in histopathologically tumor-free margins, to improve diagnosis of patients at 
      risk. METHODS: 42 patients with firstly diagnosed, radically resected primary 
      OSCC with histopathologically confirmed tumor-free resection margins (treated 
      between 1994 and 2003) were included. Inclusion criteria comprised of follow-up 
      ⩾5years, and radical surgery without postoperative treatment. Formalin-fixed 
      paraffine-embedded tissue sections of 42 tumors, 290 resection margins, and 11 
      recurrences were subjected to fluorescence in situ hybridization (FISH) to 
      examine chromosome 1 and 7 copy number variations (CNV), and to p53 
      immunohistochemistry (IHC). RESULTS: 11 out of the 42 patients developed a local 
      recurrence within 5years. FISH analysis showed that nine of eleven recurrences 
      exhibited CI in at least one of the resection margins (p=0.008). P53 
      overexpression and routine histopathologic classification were not correlated 
      with recurrent disease. The presence of CI in the resection margins revealed a 
      significantly worse progression-free survival (log-rank p=0.012). CONCLUSIONS: CI 
      in the resection margins of OSCC can reliably identify patients at risk for 
      developing a local recurrence.
CI  - Copyright (c) 2016 Elsevier Ltd. All rights reserved.
FAU - Pierssens, Damiana D C G
AU  - Pierssens DDCG
AD  - Department of Oral and Craniomaxillofacial Surgery, GROW-School for Oncology and 
      Developmental Biology, Maastricht University Medical Centre, Maastricht, The 
      Netherlands. Electronic address: damiana.pierssens@mumc.nl.
FAU - Borgemeester, Maarten C
AU  - Borgemeester MC
AD  - Department of Otorhinolaryngology, Head & Neck Surgery, GROW-School for Oncology 
      and Developmental Biology, Maastricht University Medical Centre, Maastricht, The 
      Netherlands.
FAU - van der Heijden, Stijn J H
AU  - van der Heijden SJH
AD  - Department of Otorhinolaryngology, Head & Neck Surgery, GROW-School for Oncology 
      and Developmental Biology, Maastricht University Medical Centre, Maastricht, The 
      Netherlands.
FAU - Peutz-Kootstra, Carine J
AU  - Peutz-Kootstra CJ
AD  - Department of Pathology, GROW-School for Oncology and Developmental Biology, 
      Maastricht University Medical Centre, Maastricht, The Netherlands.
FAU - Ruland, Andrea M
AU  - Ruland AM
AD  - Department of Pathology, GROW-School for Oncology and Developmental Biology, 
      Maastricht University Medical Centre, Maastricht, The Netherlands.
FAU - Haesevoets, Annick M
AU  - Haesevoets AM
AD  - Department of Pathology, GROW-School for Oncology and Developmental Biology, 
      Maastricht University Medical Centre, Maastricht, The Netherlands.
FAU - Kessler, Peter A W H
AU  - Kessler PAWH
AD  - Department of Oral and Craniomaxillofacial Surgery, GROW-School for Oncology and 
      Developmental Biology, Maastricht University Medical Centre, Maastricht, The 
      Netherlands.
FAU - Kremer, Bernd
AU  - Kremer B
AD  - Department of Otorhinolaryngology, Head & Neck Surgery, GROW-School for Oncology 
      and Developmental Biology, Maastricht University Medical Centre, Maastricht, The 
      Netherlands.
FAU - Speel, Ernst-Jan M
AU  - Speel EM
AD  - Department of Pathology, GROW-School for Oncology and Developmental Biology, 
      Maastricht University Medical Centre, Maastricht, The Netherlands.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170106
PL  - England
TA  - Oral Oncol
JT  - Oral oncology
JID - 9709118
SB  - IM
MH  - Carcinoma, Squamous Cell/*genetics/pathology/surgery
MH  - *Chromosomal Instability
MH  - Chromosomes, Human, Pair 1
MH  - Chromosomes, Human, Pair 7
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Mouth Neoplasms/*genetics/pathology/surgery
MH  - *Neoplasm Recurrence, Local
OTO - NOTNLM
OT  - Aneuploidy
OT  - Chromosomal instability
OT  - Copy number variation
OT  - FISH
OT  - Oral squamous cell carcinoma
OT  - Recurrence
OT  - Resection margins
OT  - p53
EDAT- 2017/03/03 06:00
MHDA- 2018/03/27 06:00
CRDT- 2017/03/03 06:00
PHST- 2016/09/25 00:00 [received]
PHST- 2016/12/08 00:00 [revised]
PHST- 2016/12/28 00:00 [accepted]
PHST- 2017/03/03 06:00 [entrez]
PHST- 2017/03/03 06:00 [pubmed]
PHST- 2018/03/27 06:00 [medline]
AID - S1368-8375(16)30276-7 [pii]
AID - 10.1016/j.oraloncology.2016.12.029 [doi]
PST - ppublish
SO  - Oral Oncol. 2017 Mar;66:14-21. doi: 10.1016/j.oraloncology.2016.12.029. Epub 2017 
      Jan 6.