PMID- 28253590
OWN - NLM
STAT- MEDLINE
DCOM- 20170801
LR  - 20211204
IS  - 1002-0098 (Print)
IS  - 1002-0098 (Linking)
VI  - 52
IP  - 2
DP  - 2017 Feb 9
TI  - [Activation of endoplasmic reticulum stress and its effect on osteogenic 
      differentiation induced by micropit/nanotube topography].
PG  - 126-131
LID - 10.3760/cma.j.issn.1002-0098.2017.02.015 [doi]
AB  - Objective: To explore the activation of endoplasmic reticulum stress (ERS) in 
      bone marrow mesenchymal stem cell (BMMSC) and its effect on osteogenic 
      differentiation induced by micropit/nanotube topography (MNT), so as to provide 
      guidance for the topography design of biomaterials. Methods: Four sample groups 
      were fabricated: polishing control group (polished titanium, PT, no treatment), 
      thapsigargin treatment (TG, 0.1 mumol/L TG treated for 9 h), MNT5 and MNT20 
      (anodized at 5 V and 20 V after acid etching). Scanning electron microscope (SEM) 
      was used to observe the topography of Ti samples. The alkaline phosphatase (ALP) 
      production, collagen secretion and extracellular matrix (ECM) mineralization of 
      BMMSC (osteogenic induced for 7, 14 and 21 d) on Ti samples were detected to 
      evaluate the osteogenic differentiation. After 12 h incubation, the shape and 
      size of ER was examined using a transmission electron microscope (TEM), and 
      ERS-related genes including immunoglobulin heavy chain binding protein (BiP), 
      protein kinase RNA-like endoplasmic reticulum kinase (PERK) and activating 
      transcription factor 4 (ATF4) were detected by quantitative real-time PCR 
      (qRT-PCR). Results: After 7, 14 and 21 d of induction, the ALP production, 
      collagen secretion and ECM mineralization in TG and MNT20 all significantly 
      increased compared to PT (P<0.05). The cells grown on TG, MNT5 and MNT20 surfaces 
      displayed gross distortions of the ER. Compared to PT, BiP, PERK, ATF4 mRNA 
      expression in TG was respectively 1.87+/-0.10, 2.24+/-0.35, 1.85+/-0.14; BiP, ATF4 mRNA 
      expression in MNT5 were respectively 1.27+/-0.09, 1.25+/-0.04; BiP, PERK, ATF4 mRNA 
      expression in MNT20 were respectively 1.44+/-0.09, 2.40+/-0.60, 1.48+/-0.05 (P<0.05). 
      Conclusions: MNT triggered different degree of ERS, and the activated ERS may 
      promote MNT-induced osteogenic differentiation.
FAU - Shi, M Q
AU  - Shi MQ
AD  - Department of Prosthodontics, School of Stomatology, The Fourth Military Medical 
      University & State Key Laboratory of Military Stomatology & National Clinical 
      Research Center for Oral Diseases & Shaanxi Key Laboratory of Oral Diseases, 
      Xi'an 710032, China.
FAU - Song, W
AU  - Song W
AD  - Department of Prosthodontics, School of Stomatology, The Fourth Military Medical 
      University & State Key Laboratory of Military Stomatology & National Clinical 
      Research Center for Oral Diseases & Shaanxi Key Laboratory of Oral Diseases, 
      Xi'an 710032, China.
FAU - Han, T X
AU  - Han TX
AD  - Department of Prosthodontics, Capital Medical University School of Stomatology, 
      Beijing 100050, China.
FAU - Chang, B
AU  - Chang B
AD  - Department of Stomatology, PLA Rocket Force General Hospital, Beijing 100088, 
      China.
FAU - Zhang, Y M
AU  - Zhang YM
AD  - Department of Prosthodontics, School of Stomatology, The Fourth Military Medical 
      University & State Key Laboratory of Military Stomatology & National Clinical 
      Research Center for Oral Diseases & Shaanxi Key Laboratory of Oral Diseases, 
      Xi'an 710032, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Kou Qiang Yi Xue Za Zhi
JT  - Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese 
      journal of stomatology
JID - 8711066
RN  - 0 (Endoplasmic Reticulum Chaperone BiP)
RN  - 0 (Heat-Shock Proteins)
RN  - 145891-90-3 (Activating Transcription Factor 4)
RN  - 67526-95-8 (Thapsigargin)
RN  - 9007-34-5 (Collagen)
RN  - EC 2.7.11.1 (PERK kinase)
RN  - EC 2.7.11.1 (eIF-2 Kinase)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Activating Transcription Factor 4/genetics/metabolism
MH  - Alkaline Phosphatase/biosynthesis
MH  - Animals
MH  - Calcification, Physiologic
MH  - Cell Differentiation/*physiology
MH  - Collagen/metabolism
MH  - Endoplasmic Reticulum Chaperone BiP
MH  - Endoplasmic Reticulum Stress/*physiology
MH  - Heat-Shock Proteins
MH  - Mesenchymal Stem Cells/*cytology
MH  - Microscopy, Electron, Scanning
MH  - *Nanotubes
MH  - Osteogenesis/*physiology
MH  - Thapsigargin/pharmacology
MH  - Time Factors
MH  - eIF-2 Kinase/genetics/metabolism
OTO - NOTNLM
OT  - Endoplasmic reticulum stress
OT  - Mesenchymal stem cells
OT  - Nanotubes
OT  - Titanium
EDAT- 2017/03/04 06:00
MHDA- 2017/08/02 06:00
CRDT- 2017/03/04 06:00
PHST- 2017/03/04 06:00 [entrez]
PHST- 2017/03/04 06:00 [pubmed]
PHST- 2017/08/02 06:00 [medline]
AID - 10.3760/cma.j.issn.1002-0098.2017.02.015 [doi]
PST - ppublish
SO  - Zhonghua Kou Qiang Yi Xue Za Zhi. 2017 Feb 9;52(2):126-131. doi: 
      10.3760/cma.j.issn.1002-0098.2017.02.015.