PMID- 2825421 OWN - NLM STAT- MEDLINE DCOM- 19880121 LR - 20190714 IS - 0042-6822 (Print) IS - 0042-6822 (Linking) VI - 161 IP - 2 DP - 1987 Dec TI - Synthesis in Escherichia coli of the HTLV-I trans-acting protein p40x. PG - 555-60 AB - The pX gene of human T-cell leukemia virus type I (HTLV-I) encodes a protein that activates the expression of viral genes in trans. Plasmid constructs designed to express the pX gene under the control of either the temperature-inducible lambda PL promoter or the trp promoter were used to transform several Escherichia coli strains, including murein-lipoprotein and Ion mutant strains. Upon induction it was possible to detect the synthesis of a new polypeptide of approximately 40 kDa which reacted specifically with serum from an ATL patient. FAU - Sanchez, A AU - Sanchez A AD - Department of Molecular Genetics, Nippon Roche Research Center, Kanagawa, Japan. FAU - Richardson, M A AU - Richardson MA FAU - Yoshida, M AU - Yoshida M FAU - Furuichi, Y AU - Furuichi Y LA - eng PT - Journal Article PL - United States TA - Virology JT - Virology JID - 0110674 RN - 0 (Nuclear Proteins) RN - 0 (Retroviridae Proteins) RN - 0 (Transcription Factors) SB - IM MH - Cloning, Molecular MH - Deltaretrovirus/*genetics/immunology MH - Escherichia coli MH - Gene Expression Regulation MH - Immunosorbent Techniques MH - Nuclear Proteins/*genetics MH - Plasmids MH - Retroviridae Proteins/*genetics/immunology MH - Transcription Factors/*genetics EDAT- 1987/12/01 00:00 MHDA- 1987/12/01 00:01 CRDT- 1987/12/01 00:00 PHST- 1987/12/01 00:00 [pubmed] PHST- 1987/12/01 00:01 [medline] PHST- 1987/12/01 00:00 [entrez] AID - 10.1016/0042-6822(87)90151-6 [doi] PST - ppublish SO - Virology. 1987 Dec;161(2):555-60. doi: 10.1016/0042-6822(87)90151-6.