PMID- 28254289
OWN - NLM
STAT- MEDLINE
DCOM- 20170614
LR  - 20220321
IS  - 1873-0191 (Electronic)
IS  - 0928-4931 (Linking)
VI  - 74
DP  - 2017 May 1
TI  - A combination of GDNF and hUCMSC transplantation loaded on SF/AGs composite 
      scaffolds for spinal cord injury repair.
PG  - 230-237
LID - S0928-4931(16)30956-0 [pii]
LID - 10.1016/j.msec.2016.12.017 [doi]
AB  - Spinal cord injury (SCI) is a severe trauma for which no effective treatment is 
      currently available. In this study, a composited treatment system was prepared 
      using a silk fibroin/alginates/glial cell line-derived neurotrophic factor 
      (SF/AGs/GDNF) scaffold seeded with human umbilical cord mesenchymal stem cells 
      (hUCMSCs) and the combined therapeutic effects of the composite scaffold to 
      repair SCI rats were evaluated. The use of SF as a scaffold material could act as 
      a biomimetic platform allowing neurons to properly accommodate and rebuild the 
      target tissue. The SF/AGs/GDNF scaffold had the best sustained-release function 
      and the AGs were the key determining factor in the controlled release of GDNF. 
      After 8weeks of treatment, the hUCMSCs on SF/AGs/GDNF composite scaffolds could 
      significantly enhance the scar expansion of spinal cord tissue and increased the 
      number of surviving neurons. The combination of GDNF and hUCMSCs transplantation 
      loaded on SF/AGs composite scaffolds exhibited better therapeutic and repair 
      effects to the SCI of rats, compared with the SF/AGs group or GDNF alone on 
      SF/AGs scaffolds. The composite scaffold, GDNF and stem cells could build a 
      bioactive material to form the micro-environment of growth and repair of the 
      neurons. These results may provide a theoretical basis and beneficial exploration 
      for clinical treatment of SCI.
CI  - Copyright (c) 2016 Elsevier B.V. All rights reserved.
FAU - Jiao, Genlong
AU  - Jiao G
AD  - Department of Orthopedic, The First Affiliated Hospital of Jinan University, 
      Guangzhou 510632, China.
FAU - Lou, Guofeng
AU  - Lou G
AD  - Institute of Biomedicine & Department of Cell Biology, Jinan University, 
      Guangzhou 510632, China.
FAU - Mo, Yunfei
AU  - Mo Y
AD  - Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Jinan 
      University, Guangzhou 510632, China; Department of Biomedical Engineering, Jinan 
      University, Guangzhou 510632, China.
FAU - Pan, Yongqin
AU  - Pan Y
AD  - Department of Surgery, The First Affiliated Hospital of Jinan University, 
      Guangzhou 510632, China.
FAU - Zhang, Ziyong
AU  - Zhang Z
AD  - Department of Materials Science and Engineering, Jinan University, Guangzhou 
      510632, China.
FAU - Guo, Rui
AU  - Guo R
AD  - Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Jinan 
      University, Guangzhou 510632, China; Department of Biomedical Engineering, Jinan 
      University, Guangzhou 510632, China. Electronic address: guorui@jnu.edu.cn.
FAU - Li, Zhizhong
AU  - Li Z
AD  - Department of Orthopedic, The First Affiliated Hospital of Jinan University, 
      Guangzhou 510632, China. Electronic address: lizhizhong1@medmail.com.cn.
LA  - eng
PT  - Journal Article
DEP - 20161209
PL  - Netherlands
TA  - Mater Sci Eng C Mater Biol Appl
JT  - Materials science & engineering. C, Materials for biological applications
JID - 101484109
RN  - 0 (Alginates)
RN  - 0 (Glial Cell Line-Derived Neurotrophic Factor)
SB  - IM
MH  - Alginates/*chemistry
MH  - Animals
MH  - Behavior, Animal
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Female
MH  - Glial Cell Line-Derived Neurotrophic Factor/*chemistry/metabolism
MH  - Humans
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/cytology/metabolism
MH  - Microscopy, Fluorescence
MH  - PC12 Cells
MH  - Rats
MH  - Rats, Wistar
MH  - Spinal Cord Injuries/metabolism/pathology/*therapy
MH  - Tissue Scaffolds/*chemistry
MH  - Umbilical Cord/cytology
OTO - NOTNLM
OT  - Alginate microspheres
OT  - Glial cell-derived neurotrophic factor
OT  - Human umbilical cord mesenchymal stem cells
OT  - Scaffold
OT  - Silk fibroin
OT  - Spinal cord injury
EDAT- 2017/03/04 06:00
MHDA- 2017/06/15 06:00
CRDT- 2017/03/04 06:00
PHST- 2016/08/23 00:00 [received]
PHST- 2016/10/26 00:00 [revised]
PHST- 2016/12/04 00:00 [accepted]
PHST- 2017/03/04 06:00 [entrez]
PHST- 2017/03/04 06:00 [pubmed]
PHST- 2017/06/15 06:00 [medline]
AID - S0928-4931(16)30956-0 [pii]
AID - 10.1016/j.msec.2016.12.017 [doi]
PST - ppublish
SO  - Mater Sci Eng C Mater Biol Appl. 2017 May 1;74:230-237. doi: 
      10.1016/j.msec.2016.12.017. Epub 2016 Dec 9.