PMID- 28256590
OWN - NLM
STAT- MEDLINE
DCOM- 20181106
LR  - 20181202
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 7
DP  - 2017 Mar 3
TI  - CD61 promotes the differentiation of canine ADMSCs into PGC-like cells through 
      modulation of TGF-beta signaling.
PG  - 43851
LID - 10.1038/srep43851 [doi]
LID - 43851
AB  - Previous studies have shown that CD61 (integrin-beta3) promotes the differentiation 
      of human umbilical cord mesenchymal stem cells (hUC-MSCs) into germ-like cells. 
      However, the mechanism remains unclear. In this study, we showed that 
      overexpression of CD61 in canine adipose-derived mesenchymal stem cells (cADMSCs) 
      promotes their differentiation into primordial germ cell (PGC)-like cells. 
      Quantitative real-time PCR, immunocytochemistry and western blot detected higher 
      levels of PGC-specific markers in CD61-overexpressed cADMSCs compared with those 
      in control cells. Moreover, phosphorylation of Smad2, a downstream mediator of 
      transforming growth factor beta (TGF-beta), was increased in CD61-overexpressed 
      cADMSCs than that in control cells. However, the expression of PGC-specific 
      markers was downregulated in cADMSCs treated with a TGF-beta inhibitor. These 
      results suggested that CD61 could induce cADMSCs to differentiate into PGC-like 
      cells by relying on the activation of TGF-beta pathway. ADMSCs possess a 
      considerable potential in treating the infertility of rare animal species.
FAU - Fang, Jia
AU  - Fang J
AD  - College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering 
      &Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China.
FAU - Wei, Yudong
AU  - Wei Y
AD  - College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering 
      &Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China.
FAU - Lv, Changrong
AU  - Lv C
AD  - College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering 
      &Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China.
FAU - Peng, Sha
AU  - Peng S
AD  - College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering 
      &Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China.
FAU - Zhao, Shanting
AU  - Zhao S
AD  - College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering 
      &Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China.
FAU - Hua, Jinlian
AU  - Hua J
AD  - College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering 
      &Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China.
LA  - eng
GR  - L30 AA021605/AA/NIAAA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170303
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Integrin beta3)
RN  - 0 (LY2109761)
RN  - 0 (Pyrazoles)
RN  - 0 (Pyrroles)
RN  - 0 (Smad2 Protein)
RN  - 0 (Transforming Growth Factor beta)
SB  - IM
MH  - Adipose Tissue/cytology
MH  - Animals
MH  - *Cell Differentiation
MH  - Cells, Cultured
MH  - Dogs
MH  - Gene Expression/drug effects
MH  - Germ Cells/cytology/*metabolism
MH  - Integrin beta3/genetics/*metabolism
MH  - Male
MH  - Mesenchymal Stem Cells/cytology/*metabolism
MH  - Phosphorylation
MH  - Pyrazoles/pharmacology
MH  - Pyrroles/pharmacology
MH  - Signal Transduction/drug effects
MH  - Smad2 Protein/metabolism
MH  - Transforming Growth Factor beta/*metabolism/pharmacology
PMC - PMC5335555
COIS- The authors declare no competing financial interests.
EDAT- 2017/03/04 06:00
MHDA- 2018/11/07 06:00
PMCR- 2017/03/03
CRDT- 2017/03/04 06:00
PHST- 2016/10/05 00:00 [received]
PHST- 2017/01/30 00:00 [accepted]
PHST- 2017/03/04 06:00 [entrez]
PHST- 2017/03/04 06:00 [pubmed]
PHST- 2018/11/07 06:00 [medline]
PHST- 2017/03/03 00:00 [pmc-release]
AID - srep43851 [pii]
AID - 10.1038/srep43851 [doi]
PST - epublish
SO  - Sci Rep. 2017 Mar 3;7:43851. doi: 10.1038/srep43851.