PMID- 28257842
OWN - NLM
STAT- MEDLINE
DCOM- 20170612
LR  - 20191210
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 485
IP  - 4
DP  - 2017 Apr 15
TI  - PICOT alleviates myocardial ischemia-reperfusion injury by reducing intracellular 
      levels of reactive oxygen species.
PG  - 807-813
LID - S0006-291X(17)30436-9 [pii]
LID - 10.1016/j.bbrc.2017.02.136 [doi]
AB  - Excessive generation of reactive oxygen species (ROS) is one of the main causes 
      of myocardial ischemia-reperfusion (I/R) injury. In this study, we investigated 
      the role of protein kinase C-interacting cousin of thioredoxin (PICOT; Grx3) 
      during myocardial I/R using PICOT transgenic (TG) and knockdown (KD) mice. 
      Infarction and apoptosis were attenuated in PICOT TG mice but exacerbated in 
      PICOT KD mice upon I/R. In parallel, I/R-induced generation of ROS was attenuated 
      in PICOT TG mice but exacerbated in PICOT KD mice. Angiotensin II 
      (AngII)-mediated increases in ROS and free iron levels were also attenuated in 
      cardiomyocytes isolated from PICOT TG mice but exacerbated in cardiomyocytes from 
      PICOT KD mice. Accordingly, H(2)O(2)-mediated cell death was attenuated in 
      cardiomyocytes isolated from PICOT TG mice but exacerbated in cardiomyocytes from 
      PICOT KD mice. Taken together, these data show that PICOT alleviates myocardial 
      I/R injury by regulating intracellular ROS and free iron levels. We suggest that 
      PICOT presents a novel therapeutic strategy for myocardial I/R injury.
CI  - Copyright (c) 2017 Elsevier Inc. All rights reserved.
FAU - Kim, Jihwa
AU  - Kim J
AD  - College of Life Sciences, Gwangju Institute of Science and Technology, Gwangju 
      Institute of Science and Technology, 123 Cheomdangwagi-ro, Buk-gu, Gwangju 61005, 
      Republic of Korea.
FAU - Kim, Jooyeon
AU  - Kim J
AD  - College of Life Sciences, Gwangju Institute of Science and Technology, Gwangju 
      Institute of Science and Technology, 123 Cheomdangwagi-ro, Buk-gu, Gwangju 61005, 
      Republic of Korea.
FAU - Kook, Hyun
AU  - Kook H
AD  - Department of Pharmacology and Medical Research Center for Gene Regulation, 
      Chonnam National University Medical School, 160 Baekseo-ro, Dong-ku, Gwangju 
      61469, Republic of Korea.
FAU - Park, Woo Jin
AU  - Park WJ
AD  - College of Life Sciences, Gwangju Institute of Science and Technology, Gwangju 
      Institute of Science and Technology, 123 Cheomdangwagi-ro, Buk-gu, Gwangju 61005, 
      Republic of Korea. Electronic address: woojinpark@icloud.com.
LA  - eng
PT  - Journal Article
DEP - 20170228
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Carrier Proteins)
RN  - 0 (Oxidants)
RN  - 0 (Reactive Oxygen Species)
RN  - 11128-99-7 (Angiotensin II)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - E1UOL152H7 (Iron)
RN  - EC 1.8.4.2 (Protein Disulfide Reductase (Glutathione))
RN  - EC 1.8.4.2 (Txnl2 protein, mouse)
SB  - IM
MH  - Angiotensin II/pharmacology
MH  - Animals
MH  - Carrier Proteins/genetics/*metabolism
MH  - Cell Survival/drug effects/genetics
MH  - Cells, Cultured
MH  - Dose-Response Relationship, Drug
MH  - Hydrogen Peroxide/pharmacology
MH  - Intracellular Space/drug effects/*metabolism
MH  - Iron/metabolism
MH  - Male
MH  - Mice, Knockout
MH  - Mice, Transgenic
MH  - Microscopy, Fluorescence
MH  - Myocardial Reperfusion Injury/genetics/*metabolism
MH  - Myocytes, Cardiac/cytology/drug effects/*metabolism
MH  - Oxidants/pharmacology
MH  - Protein Disulfide Reductase (Glutathione)
MH  - Reactive Oxygen Species/*metabolism
OTO - NOTNLM
OT  - Free iron
OT  - Glutaredoxin 3
OT  - Heart
OT  - Ischemia-reperfusion injury
OT  - PICOT
OT  - ROS
EDAT- 2017/03/05 06:00
MHDA- 2017/06/13 06:00
CRDT- 2017/03/05 06:00
PHST- 2017/02/22 00:00 [received]
PHST- 2017/02/27 00:00 [accepted]
PHST- 2017/03/05 06:00 [pubmed]
PHST- 2017/06/13 06:00 [medline]
PHST- 2017/03/05 06:00 [entrez]
AID - S0006-291X(17)30436-9 [pii]
AID - 10.1016/j.bbrc.2017.02.136 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2017 Apr 15;485(4):807-813. doi: 
      10.1016/j.bbrc.2017.02.136. Epub 2017 Feb 28.