PMID- 28257878
OWN - NLM
STAT- MEDLINE
DCOM- 20171215
LR  - 20180315
IS  - 1873-4596 (Electronic)
IS  - 0891-5849 (Linking)
VI  - 106
DP  - 2017 May
TI  - The carboxyl terminus of heat shock protein 70-interacting protein (CHIP) 
      participates in high glucose-induced cardiac injury.
PG  - 339-344
LID - S0891-5849(17)30119-3 [pii]
LID - 10.1016/j.freeradbiomed.2017.02.047 [doi]
AB  - The carboxyl terminus of heat shock protein 70-interacting protein (CHIP) is 
      confirmed to have a protective effect on the myocardium, but its effect on 
      diabetic cardiomyopathy is unclear. Small interfering RNA (siRNA) was used for 
      knockdown experiments in neonatal rat cardiomyocytes to examine the function of 
      CHIP in high glucose-induced injury. High glucose stimulated the production of 
      reactive oxygen species (ROS), nicotinamide adenine dinucleotide phosphate 
      oxidase (NOX), interleukin-1beta (IL-1beta), interleukin-6 (IL-6), and monocyte 
      chemoattractant protein-1 (MCP-1) production. However, cardiomyocytes lacking 
      CHIP suffered from increased oxidative stress and inflammatory responses. High 
      glucose increased the expression of Bax and caspase-3 mRNAs, decreased the 
      expression of Bcl-2 mRNA, and up-regulated the expression of the nuclear factor 
      kappa B (NF-kappaB) and mitogen-activated protein kinase (MAPK) proteins. However, 
      upon CHIP knockdown, the expression of Bax and caspase-3 mRNAs increased even 
      further, and the expression of Bcl-2 mRNA was further suppressed. The expression 
      of the phosphorylated p65 and p38 proteins (p-p65 and p-p38) was also further 
      enhanced. Thus, CHIP is a potent cardioprotective molecule.
CI  - Copyright (c) 2017 Elsevier Inc. All rights reserved.
FAU - Xiong, Wenjun
AU  - Xiong W
AD  - Department of Cardiology, The First Affiliated Hospital of Nanchang University, 
      Nanchang, Jiangxi 330006, People's Republic of China.
FAU - Liu, Shiwen
AU  - Liu S
AD  - Department of Cardiology, The First Affiliated Hospital of Nanchang University, 
      Nanchang, Jiangxi 330006, People's Republic of China.
FAU - Cai, Wenyao
AU  - Cai W
AD  - Department of Cardiology, The First Affiliated Hospital of Nanchang University, 
      Nanchang, Jiangxi 330006, People's Republic of China.
FAU - Wen, Jinhua
AU  - Wen J
AD  - Department of Cardiology, The First Affiliated Hospital of Nanchang University, 
      Nanchang, Jiangxi 330006, People's Republic of China.
FAU - Fu, Yongnan
AU  - Fu Y
AD  - Department of Cardiology, The First Affiliated Hospital of Nanchang University, 
      Nanchang, Jiangxi 330006, People's Republic of China. Electronic address: 
      fyn1028@126.com.
FAU - Peng, Jingtian
AU  - Peng J
AD  - Department of Cardiology, The First Affiliated Hospital of Nanchang University, 
      Nanchang, Jiangxi 330006, People's Republic of China.
FAU - Zheng, Zeqi
AU  - Zheng Z
AD  - Department of Cardiology, The First Affiliated Hospital of Nanchang University, 
      Nanchang, Jiangxi 330006, People's Republic of China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170228
PL  - United States
TA  - Free Radic Biol Med
JT  - Free radical biology & medicine
JID - 8709159
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Nucleocytoplasmic Transport Proteins)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (bcl-2-Associated X Protein)
RN  - 0 (p65 oncofetal mRNA transport protein, rat)
RN  - EC 2.3.2.27 (STUB1 protein, human)
RN  - EC 2.3.2.27 (Ubiquitin-Protein Ligases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Diabetic Cardiomyopathies/*genetics/metabolism/pathology
MH  - Gene Expression Regulation
MH  - Glucose/toxicity
MH  - Heart Injuries/chemically induced/*genetics/physiopathology
MH  - Humans
MH  - Myocytes, Cardiac/metabolism/pathology
MH  - Neoplasm Proteins/*genetics
MH  - Nucleocytoplasmic Transport Proteins/*genetics
MH  - Oxidative Stress/genetics
MH  - Proto-Oncogene Proteins c-bcl-2/biosynthesis/genetics
MH  - Rats
MH  - Reactive Oxygen Species/metabolism
MH  - Ubiquitin-Protein Ligases/*genetics
MH  - bcl-2-Associated X Protein/biosynthesis/genetics
MH  - p38 Mitogen-Activated Protein Kinases/*genetics
OTO - NOTNLM
OT  - CHIP
OT  - Cardiomyocytes
OT  - High glucose
OT  - NF-kappaB
OT  - ROS
EDAT- 2017/03/05 06:00
MHDA- 2017/12/16 06:00
CRDT- 2017/03/05 06:00
PHST- 2016/11/24 00:00 [received]
PHST- 2017/02/21 00:00 [revised]
PHST- 2017/02/27 00:00 [accepted]
PHST- 2017/03/05 06:00 [pubmed]
PHST- 2017/12/16 06:00 [medline]
PHST- 2017/03/05 06:00 [entrez]
AID - S0891-5849(17)30119-3 [pii]
AID - 10.1016/j.freeradbiomed.2017.02.047 [doi]
PST - ppublish
SO  - Free Radic Biol Med. 2017 May;106:339-344. doi: 
      10.1016/j.freeradbiomed.2017.02.047. Epub 2017 Feb 28.