PMID- 28260093
OWN - NLM
STAT- MEDLINE
DCOM- 20170515
LR  - 20181113
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Print)
IS  - 1791-2997 (Linking)
VI  - 15
IP  - 4
DP  - 2017 Apr
TI  - Recombinant adeno-associated virus serotype 9 in a mouse model of 
      atherosclerosis: Determination of the optimal expression time in vivo.
PG  - 2090-2096
LID - 10.3892/mmr.2017.6235 [doi]
AB  - Adeno-associated virus 9 (AAV9) has been identified as one of the optimal gene 
      transduction carriers for gene therapy. The aim of the present study was to 
      determine the gene transfection efficiency and safety of an AAV9 vector produced 
      using a recombinant baculovirus (rBac)‑based system. AAV9‑cytomegalovirus 
      (CMV)-green fluorescent protein was produced using an rBac system and the 
      resulting vector particles were injected intravenously into mice. Animals were 
      sacrificed at 14, 21, 28, 35, 60, 90 and 120 days following injection. GFP 
      expression in aortic vasculature and aortic plaques in C57/6B and apolipoprotein 
      E‑/‑ mice was analyzed by fluorescence imaging and western blotting. In vivo 
      analyses of biological markers of liver and heart damage, and renal function, as 
      well as in vitro terminal deoxynucleotidyl transferase dUTP nick end labeling 
      analysis were used to determine the toxicity of the AAV9 carrier. The findings of 
      the present study demonstrated that AAV9 viral vectors packaged using the rBac 
      system functioned appropriately in arteriosclerosis plaques. The CMV promoter 
      significantly induced GFP expression in the vascular plaque in a time-dependent 
      manner. AAV9‑CMV viral particles did not lead to heart, liver or renal damage and 
      no change in apoptotic rate was identified. These findings indicated that 
      AAV9-CMV may be effectively and safely used to transfect genes into 
      atherosclerotic plaques.
FAU - Chen, Qingjie
AU  - Chen Q
AD  - Department of Cardiology, First Affiliated Hospital of Xinjiang Medical 
      University, Urumqi, Xinjiang 830054, P.R. China.
FAU - Zhai, Hui
AU  - Zhai H
AD  - Department of Cardiology, First Affiliated Hospital of Xinjiang Medical 
      University, Urumqi, Xinjiang 830054, P.R. China.
FAU - Li, Xiaomei
AU  - Li X
AD  - Department of Cardiology, First Affiliated Hospital of Xinjiang Medical 
      University, Urumqi, Xinjiang 830054, P.R. China.
FAU - Ma, Yitong
AU  - Ma Y
AD  - Department of Cardiology, First Affiliated Hospital of Xinjiang Medical 
      University, Urumqi, Xinjiang 830054, P.R. China.
FAU - Chen, Bangdang
AU  - Chen B
AD  - Xinjiang Key Laboratory of Cardiovascular Disease Research, Urumqi, Xinjiang 
      830054, P.R. China.
FAU - Liu, Fen
AU  - Liu F
AD  - Xinjiang Key Laboratory of Cardiovascular Disease Research, Urumqi, Xinjiang 
      830054, P.R. China.
FAU - Lai, Hongmei
AU  - Lai H
AD  - Department of Cardiology, First Affiliated Hospital of Xinjiang Medical 
      University, Urumqi, Xinjiang 830054, P.R. China.
FAU - Xie, Jia
AU  - Xie J
AD  - Department of Cardiology, First Affiliated Hospital of Xinjiang Medical 
      University, Urumqi, Xinjiang 830054, P.R. China.
FAU - He, Chunhui
AU  - He C
AD  - Department of Cardiology, First Affiliated Hospital of Xinjiang Medical 
      University, Urumqi, Xinjiang 830054, P.R. China.
FAU - Luo, Junyi
AU  - Luo J
AD  - Department of Cardiology, First Affiliated Hospital of Xinjiang Medical 
      University, Urumqi, Xinjiang 830054, P.R. China.
FAU - Gao, Jing
AU  - Gao J
AD  - Department of Cardiology, First Affiliated Hospital of Xinjiang Medical 
      University, Urumqi, Xinjiang 830054, P.R. China.
FAU - Yang, Yining
AU  - Yang Y
AD  - Department of Cardiology, First Affiliated Hospital of Xinjiang Medical 
      University, Urumqi, Xinjiang 830054, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20170222
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Animals
MH  - Aorta/metabolism/pathology
MH  - Atherosclerosis/*genetics/pathology/therapy
MH  - Baculoviridae/genetics
MH  - Cytomegalovirus/genetics
MH  - Dependovirus/*genetics
MH  - Disease Models, Animal
MH  - Genetic Therapy
MH  - Genetic Vectors/*administration & dosage/analysis/*genetics
MH  - Green Fluorescent Proteins/analysis/genetics
MH  - Male
MH  - Mice, Inbred C57BL
MH  - Plaque, Atherosclerotic/*genetics/pathology/therapy
MH  - Promoter Regions, Genetic
MH  - Transduction, Genetic
MH  - Transfection/*methods
PMC - PMC5364991
EDAT- 2017/03/06 06:00
MHDA- 2017/05/16 06:00
PMCR- 2017/02/22
CRDT- 2017/03/06 06:00
PHST- 2015/11/23 00:00 [received]
PHST- 2016/11/23 00:00 [accepted]
PHST- 2017/03/06 06:00 [pubmed]
PHST- 2017/05/16 06:00 [medline]
PHST- 2017/03/06 06:00 [entrez]
PHST- 2017/02/22 00:00 [pmc-release]
AID - mmr-15-04-2090 [pii]
AID - 10.3892/mmr.2017.6235 [doi]
PST - ppublish
SO  - Mol Med Rep. 2017 Apr;15(4):2090-2096. doi: 10.3892/mmr.2017.6235. Epub 2017 Feb 
      22.