PMID- 28260296
OWN - NLM
STAT- MEDLINE
DCOM- 20180531
LR  - 20211204
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 97
IP  - 7
DP  - 2017 Feb 21
TI  - [Expression of signaling pathway of mammalian target of rapamycin in articular 
      cartilage cell induced by interleukin 1beta].
PG  - 540-544
LID - 10.3760/cma.j.issn.0376-2491.2017.07.014 [doi]
AB  - Objective: To observe effect of interleukin(IL)-1beta on the expression of signaling 
      pathway of mammalian target of rapamycin(mTOR) of articular cartilage. Methods: 
      Articular cartilage of rats was isolated under sterile technique, cells were 
      digested by type Ⅱ collagenase and trypsin and cultured in vitro, pre-culture the 
      Ⅱ cells for three days, different concentrations of IL-1beta were added for 24 
      hours.The cells were stained with toluidine blue and HE, to observe morphological 
      changes of cells.RT-PCR was used to detect the mRNA expression of typeⅡcollagen 
      gene, aggrecan gene, mTOR gene and P70S6K gene, Western blotting was used to 
      detect the expression of protein related to mTOR. Results: With increasing 
      concentrations of IL-1beta, the phenotype of cells appeared polygon into a spindle, 
      the mRNA expression of gene of type Ⅱ collagen (the control group: 0.821+/-0.014; 1 
      ng/ml: 0.614+/-0.014; 10 ng/ml: 0.549+/-0.009; 100 ng/ml: 0.520+/-0.008), 
      aggrecan(0.867+/-0.005; 0.857+/-0.001; 0.554+/-0.008; 0.538+/-0.004) and 
      mTOR(0.845+/-0.015; 0.785+/-0.009; 0.569+/-0.025; 0.518+/-0.014) reduced, but 
      P70S6K(0.465+/-0.024; 0.566+/-0.022; 0.663+/-0.022; 0.896+/-0.015) increased by PCR 
      .Expression of protein detected by Western blotting was similar to the trend of 
      PCR. Conclusion: mTOR signaling pathway may play an important role on the 
      degeneration of articular cartilage, regulating mTOR signaling pathway may 
      provides a new idea of delaying the degeneration process of cells.
FAU - Xiong, S L
AU  - Xiong SL
AD  - Department of Arthrosis Surgery, Yijishan Hosptial, Wannan Medical College, Anhui 
      241011, China.
FAU - Xu, H G
AU  - Xu HG
FAU - Wang, Q
AU  - Wang Q
FAU - Wang, J
AU  - Wang J
FAU - He, H Z
AU  - He HZ
FAU - Yang, X
AU  - Yang X
FAU - Dong, L J
AU  - Dong LJ
FAU - Zhao, Q L
AU  - Zhao QL
FAU - Huang, D G
AU  - Huang DG
FAU - Xuan, H B
AU  - Xuan HB
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
RN  - 0 (Aggrecans)
RN  - 0 (Collagen Type II)
RN  - 0 (Interleukin-1beta)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases, 70-kDa)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 2.7.11.1 (ribosomal protein S6 kinase, 70kD, polypeptide 2)
RN  - W36ZG6FT64 (Sirolimus)
SB  - IM
MH  - Aggrecans
MH  - Animals
MH  - *Cartilage, Articular
MH  - Cells, Cultured
MH  - Chondrocytes
MH  - Collagen Type II
MH  - Interleukin-1beta
MH  - Rats
MH  - Ribosomal Protein S6 Kinases, 70-kDa
MH  - *Signal Transduction
MH  - Sirolimus
MH  - TOR Serine-Threonine Kinases
OTO - NOTNLM
OT  - Cartilage, articular
OT  - Cell aging
OT  - Interleukin-1
OT  - Protein kinases
OT  - mTOR
EDAT- 2017/03/06 06:00
MHDA- 2018/06/01 06:00
CRDT- 2017/03/06 06:00
PHST- 2017/03/06 06:00 [entrez]
PHST- 2017/03/06 06:00 [pubmed]
PHST- 2018/06/01 06:00 [medline]
AID - 10.3760/cma.j.issn.0376-2491.2017.07.014 [doi]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2017 Feb 21;97(7):540-544. doi: 
      10.3760/cma.j.issn.0376-2491.2017.07.014.