PMID- 28260507
OWN - NLM
STAT- MEDLINE
DCOM- 20180322
LR  - 20191210
IS  - 1875-5739 (Electronic)
IS  - 1567-2026 (Print)
IS  - 1567-2026 (Linking)
VI  - 14
IP  - 2
DP  - 2017
TI  - Tanshinone IIA Protects Hippocampal Neuronal Cells from Reactive Oxygen Species 
      Through Changes in Autophagy and Activation of Phosphatidylinositol 3-Kinase, 
      Protein Kinas B, and Mechanistic Target of Rapamycin Pathways.
PG  - 132-140
LID - 10.2174/1567202614666170306105315 [doi]
AB  - BACKGROUND: Tanshinone IIA is a key active ingredient of danshen, which is 
      derived from the dried root or rhizome of Salviae miltiorrhizae Bge. The 
      tanshinone IIA has protective effects against the focal cerebral ischemic injury. 
      However, the underlying mechanisms remain unclear. METHODS: An in vitro model of 
      cerebral ischemia was established by subjecting cultures of hippocampal neuronal 
      cells to oxygen-glucose deprivation followed by reperfusion (OGD/R). The probes 
      of 5-(and-6)-chloromethyl-2',7'-dichlorodihydrofluorescein diacetate, acetyl 
      ester (CMH2DCFDA) and 
      5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolylcarbocyanine,iodide (JC-1) 
      were used to determine the mitochondrial membrane potential (MMP) and reactive 
      oxygen species (ROS) production. Western-blot was used to detect the expression 
      of proteins in HT-22 cells. RESULTS: The results of cell proliferative assays 
      showed that the tanshinone IIA attenuated OGD/Rmediated neuronal cell death, with 
      the evidence of increased cell viability. In addition, OGD/R exposure led to 
      increase the levels of intracellular reactive oxygen species (ROS), which were 
      significantly suppressed by tanshinone IIA treatment. Furthermore, tanshinone IIA 
      treatment inhibited elevations in MMP and autophagy following exposure to OGD/R. 
      Additionally, OGD/R promoted cell death with concomitant inhibiting 
      phosphatidylinositol-3-kinase (PI3K)/protein kinase B (Akt)/ mammalian target of 
      Rapamycin (mTOR) pathway, which was reversed by tanshinone IIA. CONCLUSION: These 
      results suggest that the tanshinone IIA protects against OGD/R-mediated cell 
      death in HT-22 cells, in part, due to activating PI3K/Akt/mTOR pathway.
CI  - Copyright(c) Bentham Science Publishers; For any queries, please email at 
      epub@benthamscience.org.
FAU - Zhu, Yingchun
AU  - Zhu Y
AD  - Department of Neurology Disease, the Affiliated Anhui Provincial Hospital of 
      Anhui Medical University, Hefei 230022, Anhui, China.
FAU - Tang, Qiqiang
AU  - Tang Q
AD  - Department of Neurology Disease, the Affiliated Anhui Provincial Hospital of 
      Anhui Medical University, Hefei 230022, Anhui, China.
FAU - Wang, Guopin
AU  - Wang G
AD  - Department of Neurology Disease, the Affiliated Anhui Provincial Hospital of 
      Anhui Medical University, Hefei 230022, Anhui, China.
FAU - Han, Ruodong
AU  - Han R
AD  - Department of Intensive Care Division, The People's Hospital of Bozhou, Bozhou 
      236800, Anhui, China.
LA  - eng
PT  - Journal Article
PL  - United Arab Emirates
TA  - Curr Neurovasc Res
JT  - Current neurovascular research
JID - 101208439
RN  - 0 (Abietanes)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Reactive Oxygen Species)
RN  - 03UUH3J385 (tanshinone)
RN  - EC 2.7.1.137 (Phosphatidylinositol 3-Kinase)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - W36ZG6FT64 (Sirolimus)
SB  - IM
MH  - Abietanes/chemistry/*pharmacology
MH  - Animals
MH  - Autophagy/*drug effects
MH  - Cell Line, Transformed
MH  - Cell Survival/drug effects
MH  - Hippocampus/cytology
MH  - Membrane Potential, Mitochondrial/drug effects
MH  - Mice
MH  - Neurons/*drug effects
MH  - Neuroprotective Agents/chemistry/*pharmacology
MH  - Phosphatidylinositol 3-Kinase/metabolism
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - Reactive Oxygen Species/*metabolism
MH  - Signal Transduction/*drug effects
MH  - Sirolimus/metabolism
PMC - PMC5543574
OTO - NOTNLM
OT  - Tanshinone IIA
OT  - cerebral ischemic
OT  - mitochondrial membrane potential
OT  - neural cells
OT  - reactive oxygen species
EDAT- 2017/03/07 06:00
MHDA- 2018/03/23 06:00
PMCR- 2017/08/04
CRDT- 2017/03/07 06:00
PHST- 2017/01/24 00:00 [received]
PHST- 2017/02/18 00:00 [revised]
PHST- 2017/02/20 00:00 [accepted]
PHST- 2017/03/07 06:00 [pubmed]
PHST- 2018/03/23 06:00 [medline]
PHST- 2017/03/07 06:00 [entrez]
PHST- 2017/08/04 00:00 [pmc-release]
AID - CNR-EPUB-82130 [pii]
AID - CNR-14-132 [pii]
AID - 10.2174/1567202614666170306105315 [doi]
PST - ppublish
SO  - Curr Neurovasc Res. 2017;14(2):132-140. doi: 10.2174/1567202614666170306105315.