PMID- 28261004
OWN - NLM
STAT- MEDLINE
DCOM- 20170807
LR  - 20200928
IS  - 2694-0604 (Electronic)
IS  - 1557-170X (Print)
IS  - 2375-7477 (Linking)
VI  - 2016
DP  - 2016 Aug
TI  - In vivo characterization of genetic expression of virus-transduced calcium 
      indicators in retinal ganglion cells using a low-cost funduscope.
PG  - 1316-1319
LID - 10.1109/EMBC.2016.7590949 [doi]
AB  - Virus-transduced calcium indicators are effective reporters of neural activity, 
      offering the advantage of cell-specific labeling. To track the level of in vivo 
      expression of genetically encoded calcium indicators (GECIs) in rodent retina, we 
      developed a noninvasive imaging approach based on a custom-modified low-cost and 
      simple fundus system that enabled us to monitor and characterize in vivo 
      bright-field and fluorescence retinal image. The system clearly resolves 
      individual retinal ganglion cells (RGCs) and axons. RGC fluorescence intensity 
      and number of observable fluorescent cells show a consistent rising trend from 
      week 1 to week 3 after viral injection, indicating a uniform increase of GCaMP6f 
      expression. At defined time points, we prepared wholemount retina mounted on a 
      transparent multielectrode array (MEA) and used calcium imaging to identify the 
      optimal time for studying the responsiveness of RGCs to external electrical 
      stimulation. The results show that the fluorescence-endoscopy fundus system is a 
      powerful and widely accessible tool for evaluating in vivo fluorescence reporter 
      expression.
FAU - Chang, Yao-Chuan
AU  - Chang YC
AD  - University of Southern California, Los Angeles, ca 90033, USA.
FAU - Walston, Steven T
AU  - Walston ST
AD  - University of Southern California, Los Angeles, ca 90033, USA.
FAU - Chow, Robert H
AU  - Chow RH
AD  - University of Southern California, Los Angeles, ca 90033, USA.
FAU - Weiland, James D
AU  - Weiland JD
AD  - University of Southern California, Los Angeles, ca 90033, USA.
LA  - eng
GR  - R01 EY022931/EY/NEI NIH HHS/United States
PT  - Journal Article
PL  - United States
TA  - Annu Int Conf IEEE Eng Med Biol Soc
JT  - Annual International Conference of the IEEE Engineering in Medicine and Biology 
      Society. IEEE Engineering in Medicine and Biology Society. Annual International 
      Conference
JID - 101763872
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Axons
MH  - Calcium
MH  - Ophthalmoscopes
MH  - Retina
MH  - *Retinal Ganglion Cells
PMC - PMC5325050
MID - NIHMS840618
EDAT- 2017/03/07 06:00
MHDA- 2017/08/08 06:00
PMCR- 2017/08/01
CRDT- 2017/03/07 06:00
PHST- 2017/03/07 06:00 [entrez]
PHST- 2017/03/07 06:00 [pubmed]
PHST- 2017/08/08 06:00 [medline]
PHST- 2017/08/01 00:00 [pmc-release]
AID - 10.1109/EMBC.2016.7590949 [doi]
PST - ppublish
SO  - Annu Int Conf IEEE Eng Med Biol Soc. 2016 Aug;2016:1316-1319. doi: 
      10.1109/EMBC.2016.7590949.