PMID- 28261150
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1664-2295 (Print)
IS  - 1664-2295 (Electronic)
IS  - 1664-2295 (Linking)
VI  - 8
DP  - 2017
TI  - Intranasal Administration of the Antisecretory Peptide AF-16 Reduces Edema and 
      Improves Cognitive Function Following Diffuse Traumatic Brain Injury in the Rat.
PG  - 39
LID - 10.3389/fneur.2017.00039 [doi]
LID - 39
AB  - A synthetic peptide with antisecretory activity, antisecretory factor (AF)-16, 
      improves injury-related deficits in water and ion transport and decreases 
      intracranial pressure after experimental cold lesion injury and encephalitis 
      although its role in traumatic brain injury (TBI) is unknown. AF-16 or an 
      inactive reference peptide was administrated intranasally 30 min following 
      midline fluid percussion injury (mFPI; n = 52), a model of diffuse mild-moderate 
      TBI in rats. Sham-injured (n = 14) or naive (n = 24) animals were used as 
      controls. The rats survived for either 48 h or 15 days post-injury. At 48 h, the 
      animals were tested in the Morris water maze (MWM) for memory function and their 
      brains analyzed for cerebral edema. Here, mFPI-induced brain edema compared to 
      sham or naive controls that was significantly reduced by AF-16 treatment 
      (p < 0.05) although MWM performance was not altered. In the 15-day survival 
      groups, the MWM learning and memory abilities as well as histological changes 
      were analyzed. AF-16-treated brain-injured animals shortened both MWM latency and 
      swim path in the learning trials (p < 0.05) and improved probe trial performance 
      compared to brain-injured controls treated with the inactive reference peptide. A 
      modest decrease by AF-16 on TBI-induced changes in hippocampal glial acidic 
      fibrillary protein (GFAP) staining (p = 0.11) was observed. AF-16 treatment did 
      not alter any other immunohistochemical analyses (degenerating neurons, 
      beta-amyloid precursor protein (beta-APP), and Olig2). In conclusion, intranasal 
      AF-16-attenuated brain edema and enhanced visuospatial learning and memory 
      following diffuse TBI in the rat. Intranasal administration early post-injury of 
      a promising neuroprotective substance offers a novel treatment approach for TBI.
FAU - Clausen, Fredrik
AU  - Clausen F
AD  - Unit for Neurosurgery, Department of Neuroscience, Uppsala University , Uppsala , 
      Sweden.
FAU - Hansson, Hans-Arne
AU  - Hansson HA
AD  - Institute of Biomedicine, University of Gothenburg , Goteborg , Sweden.
FAU - Raud, Johan
AU  - Raud J
AD  - Lantmannen AS Faktor AB , Stockholm , Sweden.
FAU - Marklund, Niklas
AU  - Marklund N
AD  - Unit for Neurosurgery, Department of Neuroscience, Uppsala University , Uppsala , 
      Sweden.
LA  - eng
PT  - Journal Article
DEP - 20170214
PL  - Switzerland
TA  - Front Neurol
JT  - Frontiers in neurology
JID - 101546899
PMC - PMC5306199
OTO - NOTNLM
OT  - AF-16
OT  - cerebral edema
OT  - cognition
OT  - intranasal
OT  - neuroprotection
OT  - traumatic brain injury
EDAT- 2017/03/07 06:00
MHDA- 2017/03/07 06:01
PMCR- 2017/02/14
CRDT- 2017/03/07 06:00
PHST- 2016/10/14 00:00 [received]
PHST- 2017/01/27 00:00 [accepted]
PHST- 2017/03/07 06:00 [entrez]
PHST- 2017/03/07 06:00 [pubmed]
PHST- 2017/03/07 06:01 [medline]
PHST- 2017/02/14 00:00 [pmc-release]
AID - 10.3389/fneur.2017.00039 [doi]
PST - epublish
SO  - Front Neurol. 2017 Feb 14;8:39. doi: 10.3389/fneur.2017.00039. eCollection 2017.