PMID- 28262732
OWN - NLM
STAT- MEDLINE
DCOM- 20181108
LR  - 20181202
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 7
DP  - 2017 Mar 6
TI  - Transplantation of hUC-MSCs seeded collagen scaffolds reduces scar formation and 
      promotes functional recovery in canines with chronic spinal cord injury.
PG  - 43559
LID - 10.1038/srep43559 [doi]
LID - 43559
AB  - Spinal cord injury (SCI) can lead to locomotor deficits, and the repair of 
      chronic SCI is considered one of the most challenging clinical problems. Although 
      extensive studies have evaluated treatments for acute SCI in small animals, 
      comparatively fewer studies have been conducted on large-animal SCI in the 
      chronic phase, which is more clinically relevant. Here, we used a collagen-based 
      biomaterial, named the NeuroRegen scaffold, loaded with human umbilical 
      cord-derived mesenchymal stem cells (hUC-MSCs) in a canine chronic SCI model. To 
      generate chronic SCI, the T8 spinal cord segment was removed by complete 
      transection of the spinal cord. Two months later, glial scar tissue was removed 
      and a NeuroRegen scaffold was transplanted into the lesion area. Functionalized 
      NeuroRegen scaffold implantation promoted both locomotor recovery and endogenous 
      neurogenesis in the lesion area. Moreover, some newly generated neurons 
      successfully matured into 5-HT-positive neurons at 1 year post-injury. In 
      addition, many regenerated axon fibers in the lesion area exhibited remyelination 
      and synapse formation at 1 year post-injury in the functionalized NeuroRegen 
      scaffold group. In conclusion, the NeuroRegen scaffold functionalized with 
      hUC-MSCs is a promising potential therapeutic approach to chronic SCI that 
      promotes neuronal regeneration, reduces glial scar formation, and ultimately 
      improves locomotor recovery.
FAU - Li, Xing
AU  - Li X
AD  - State Key Laboratory of Molecular Developmental Biology, Institute of Genetics 
      and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
FAU - Tan, Jun
AU  - Tan J
AD  - Department of Neurosurgery, Xiangya Hospital, Central South University, 87 
      Xiangya Road, Changsha 410008, Hunan Province, China.
FAU - Xiao, Zhifeng
AU  - Xiao Z
AD  - State Key Laboratory of Molecular Developmental Biology, Institute of Genetics 
      and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
FAU - Zhao, Yannan
AU  - Zhao Y
AD  - State Key Laboratory of Molecular Developmental Biology, Institute of Genetics 
      and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
FAU - Han, Sufang
AU  - Han S
AD  - State Key Laboratory of Molecular Developmental Biology, Institute of Genetics 
      and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
FAU - Liu, Dingyang
AU  - Liu D
AD  - Department of Neurosurgery, Xiangya Hospital, Central South University, 87 
      Xiangya Road, Changsha 410008, Hunan Province, China.
FAU - Yin, Wen
AU  - Yin W
AD  - Department of Neurosurgery, Xiangya Hospital, Central South University, 87 
      Xiangya Road, Changsha 410008, Hunan Province, China.
FAU - Li, Jing
AU  - Li J
AD  - State Key Laboratory of Molecular Developmental Biology, Institute of Genetics 
      and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
FAU - Li, Juan
AU  - Li J
AD  - Department of Neurosurgery, Xiangya Hospital, Central South University, 87 
      Xiangya Road, Changsha 410008, Hunan Province, China.
FAU - Wanggou, Siyi
AU  - Wanggou S
AD  - Department of Neurosurgery, Xiangya Hospital, Central South University, 87 
      Xiangya Road, Changsha 410008, Hunan Province, China.
FAU - Chen, Bing
AU  - Chen B
AD  - State Key Laboratory of Molecular Developmental Biology, Institute of Genetics 
      and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
FAU - Ren, Caiping
AU  - Ren C
AD  - Cancer Research Institute, Collaborative Innovation Center for Cancer Medicine, 
      School of Basic Medical Science, Central South University, Changsha 410008, 
      China.
FAU - Jiang, Xingjun
AU  - Jiang X
AD  - Department of Neurosurgery, Xiangya Hospital, Central South University, 87 
      Xiangya Road, Changsha 410008, Hunan Province, China.
FAU - Dai, Jianwu
AU  - Dai J
AD  - State Key Laboratory of Molecular Developmental Biology, Institute of Genetics 
      and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170306
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Animals
MH  - Chronic Disease
MH  - Cicatrix/*pathology
MH  - *Collagen/metabolism
MH  - Dogs
MH  - Humans
MH  - Locomotion
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/*cytology
MH  - Nerve Regeneration
MH  - Neurogenesis
MH  - Neuroglia/metabolism/pathology
MH  - Neurons/metabolism
MH  - Spinal Cord Injuries/*pathology/*physiopathology/rehabilitation/therapy
MH  - *Tissue Scaffolds
MH  - Treatment Outcome
MH  - Umbilical Cord/*cytology
PMC - PMC5337930
COIS- The authors declare no competing financial interests.
EDAT- 2017/03/07 06:00
MHDA- 2018/11/09 06:00
PMCR- 2017/03/06
CRDT- 2017/03/07 06:00
PHST- 2016/10/10 00:00 [received]
PHST- 2017/01/25 00:00 [accepted]
PHST- 2017/03/07 06:00 [entrez]
PHST- 2017/03/07 06:00 [pubmed]
PHST- 2018/11/09 06:00 [medline]
PHST- 2017/03/06 00:00 [pmc-release]
AID - srep43559 [pii]
AID - 10.1038/srep43559 [doi]
PST - epublish
SO  - Sci Rep. 2017 Mar 6;7:43559. doi: 10.1038/srep43559.