PMID- 28262752
OWN - NLM
STAT- MEDLINE
DCOM- 20181106
LR  - 20181113
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 7
DP  - 2017 Mar 6
TI  - Highly-Exposed HIV-1 seronegative Female Commercial Sex Workers sustain in their 
      genital mucosa increased frequencies of tolerogenic myeloid and regulatory 
      T-cells.
PG  - 43857
LID - 10.1038/srep43857 [doi]
LID - 43857
AB  - We and others have shown that HIV-1 highly-exposed seronegative (HESN) female 
      commercial sex workers (CSWs) maintain low genital inflammatory conditions to 
      prevent HIV infection. HIV-1 interacts with toll-like receptors (TLR)-7/8 to 
      induce interferon (IFN)-alpha, an important antiviral and immunomodulatory cytokine, 
      which act together with interleukin (IL)-10, human leukocyte antigen (HLA)-G and 
      immunoglobulin-like transcript (ILT)-4 to initiate a "tolerogenic/regulatory" 
      anti-inflammatory loop. In view of further unravelling elements associated with 
      natural immunity to HIV-1, we have characterised TLR-7, IFN-alpha, IL-10, HLA-G and 
      ILT-4 expression profiles in the genital tract of female CSWs and 
      HIV-1-uninfected non-CSWs from Benin. Endocervical myeloid HLA-DR(+) cells from 
      HESN CSWs expressed higher levels of IFN-alpha, TLR-7, IL-10 and HLA-G than those 
      from both HIV-1-infected CSWs and HIV-1-uninfected non-CSWs. Further 
      characterization of the endocervical myeloid HLA-DR(+) cells in HESN CSWs 
      revealed a population of "tolerogenic" CD103(+) CD14(+) CD11c(+) myeloid cells 
      expressing high levels of IFN-alpha and IL-10. Concomitantly, HESN CSWs had higher 
      frequencies of endocervical regulatory CD4(+) T-cells when compared to those from 
      the two other groups of women. These novel findings provide strong evidence to 
      support the implication of tolerogenic myeloid cells expressing high levels of 
      antiviral molecules in shaping the genital mucosal immune response to prevent HIV 
      infection.
FAU - Thibodeau, V
AU  - Thibodeau V
AD  - Laboratoire d'immunogenetique, Centre de Recherche du Centre Hospitalier de 
      l'Universite de Montreal (CRCHUM), Montreal, Canada.
AD  - Departement de Microbiologie, Infectiologie et Immunologie de l'Universite de 
      Montreal, Montreal, Canada.
FAU - Fourcade, L
AU  - Fourcade L
AD  - Laboratoire d'immunogenetique, Centre de Recherche du Centre Hospitalier de 
      l'Universite de Montreal (CRCHUM), Montreal, Canada.
AD  - Departement de Microbiologie, Infectiologie et Immunologie de l'Universite de 
      Montreal, Montreal, Canada.
FAU - Labbe, A-C
AU  - Labbe AC
AD  - Departement de Microbiologie, Infectiologie et Immunologie de l'Universite de 
      Montreal, Montreal, Canada.
FAU - Alary, M
AU  - Alary M
AD  - Centre de recherche du CHU de Quebec, Quebec, Canada.
AD  - Departement de medecine sociale et preventive, Universite Laval, Quebec, Canada.
FAU - Guedou, F
AU  - Guedou F
AD  - Dispensaire des IST, Cotonou, Benin.
FAU - Poudrier, J
AU  - Poudrier J
AD  - Laboratoire d'immunogenetique, Centre de Recherche du Centre Hospitalier de 
      l'Universite de Montreal (CRCHUM), Montreal, Canada.
AD  - Departement de Microbiologie, Infectiologie et Immunologie de l'Universite de 
      Montreal, Montreal, Canada.
FAU - Roger, M
AU  - Roger M
AD  - Laboratoire d'immunogenetique, Centre de Recherche du Centre Hospitalier de 
      l'Universite de Montreal (CRCHUM), Montreal, Canada.
AD  - Departement de Microbiologie, Infectiologie et Immunologie de l'Universite de 
      Montreal, Montreal, Canada.
LA  - eng
GR  - MOP-119406/CIHR/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170306
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Cytokines)
SB  - IM
MH  - Adult
MH  - Benin
MH  - Cytokines/immunology/metabolism
MH  - Female
MH  - Genitalia, Female/immunology/metabolism
MH  - HIV Infections/diagnosis/immunology/virology
MH  - HIV Seronegativity/*immunology
MH  - HIV-1/*immunology/physiology
MH  - Humans
MH  - Immunity, Mucosal/immunology
MH  - Middle Aged
MH  - Mucous Membrane/*immunology/metabolism
MH  - Myeloid Cells/*immunology/metabolism
MH  - Sex Work/*statistics & numerical data
MH  - T-Lymphocytes, Regulatory/*immunology/metabolism
PMC - PMC5338327
COIS- The authors declare no competing financial interests.
EDAT- 2017/03/07 06:00
MHDA- 2018/11/07 06:00
PMCR- 2017/03/06
CRDT- 2017/03/07 06:00
PHST- 2016/10/12 00:00 [received]
PHST- 2017/02/01 00:00 [accepted]
PHST- 2017/03/07 06:00 [entrez]
PHST- 2017/03/07 06:00 [pubmed]
PHST- 2018/11/07 06:00 [medline]
PHST- 2017/03/06 00:00 [pmc-release]
AID - srep43857 [pii]
AID - 10.1038/srep43857 [doi]
PST - epublish
SO  - Sci Rep. 2017 Mar 6;7:43857. doi: 10.1038/srep43857.