PMID- 28263837
OWN - NLM
STAT- MEDLINE
DCOM- 20180209
LR  - 20180210
IS  - 1879-0089 (Electronic)
IS  - 0145-305X (Linking)
VI  - 73
DP  - 2017 Aug
TI  - Echinacea pupurea extracts promote murine dendritic cell maturation by activation 
      of JNK, p38 MAPK and NF-kappaB pathways.
PG  - 21-26
LID - S0145-305X(16)30459-1 [pii]
LID - 10.1016/j.dci.2017.03.002 [doi]
AB  - Dendritic cells (DCs) comprise a system of highly professional antigen presenting 
      cells (APCs) which connect innate and adaptive immunity by undergoing dramatic 
      shift in their maturation state. Phytomedicine Echinacea purpurea extracts (EE) 
      could modulate murine dendritic cell fate and function. However, the underlying 
      mechanism of EE on DCs development and maturation remains limited. In this study, 
      immature DCs were induced phenotypic maturation with up-regulated expression of 
      key accessory molecules and the phagocytic activity was decreased after being 
      treated with EE (400 mug/ml) for 48 h. We found that TLR1/2, JNK, p38-MAPK and 
      NF-kappaB pathways were activated following EE exposure. Notably, JNK activation was 
      demonstrated to be associated with increased IFN-gamma response while p38-MAPK 
      pathway exhibited immuno-regulatory effects via induction of IL-10 and TGF-beta1. 
      Furthermore, it was verified that NF-kappaB signaling was responsible for EE-induced 
      synthesis of IFN-gamma, IL-12 and TGF-beta1, but not for IL-10 induction. These results 
      indicate that EE have the immunomodulatory potency to promote both phenotypic and 
      functional maturation of BMDCs via modulating the activation of JNK, p38-MAPK and 
      NF-kappaB pathways. Our findings contributed to the current understanding of the 
      immunoregulatory function of EE and the mechanism of DCs maturation.
CI  - Copyright (c) 2017 Elsevier Ltd. All rights reserved.
FAU - Li, Yali
AU  - Li Y
AD  - Key Laboratory of Molecular Animal Nutrition and Feed Sciences, College of Animal 
      Science, Zhejiang University, Hangzhou, 310058, PR China; Animal Nutrition and 
      Human Health Laboratory, School of Life Sciences, Hunan Normal University, 
      Changsha, 410006, PR China.
FAU - Wang, Yuanyuan
AU  - Wang Y
AD  - Key Laboratory of Molecular Animal Nutrition and Feed Sciences, College of Animal 
      Science, Zhejiang University, Hangzhou, 310058, PR China.
FAU - Wu, Yanping
AU  - Wu Y
AD  - Key Laboratory of Molecular Animal Nutrition and Feed Sciences, College of Animal 
      Science, Zhejiang University, Hangzhou, 310058, PR China.
FAU - Wang, Baikui
AU  - Wang B
AD  - Key Laboratory of Molecular Animal Nutrition and Feed Sciences, College of Animal 
      Science, Zhejiang University, Hangzhou, 310058, PR China.
FAU - Chen, Xi
AU  - Chen X
AD  - International Medical Center, Zhejiang Provincial People's Hospital, Hangzhou, 
      310014, PR China.
FAU - Xu, Xin
AU  - Xu X
AD  - Key Laboratory of Molecular Animal Nutrition and Feed Sciences, College of Animal 
      Science, Zhejiang University, Hangzhou, 310058, PR China.
FAU - Chen, Hongliang
AU  - Chen H
AD  - Qilu Animal Health Products Co., Ltd., Jinan, 250100, PR China.
FAU - Li, Weifen
AU  - Li W
AD  - Key Laboratory of Molecular Animal Nutrition and Feed Sciences, College of Animal 
      Science, Zhejiang University, Hangzhou, 310058, PR China. Electronic address: 
      weifenli@zju.edu.cn.
FAU - Xu, Xiaogang
AU  - Xu X
AD  - Key Laboratory of Molecular Animal Nutrition and Feed Sciences, College of Animal 
      Science, Zhejiang University, Hangzhou, 310058, PR China. Electronic address: 
      xuxg@zju.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170302
PL  - United States
TA  - Dev Comp Immunol
JT  - Developmental and comparative immunology
JID - 7708205
RN  - 0 (NF-kappa B)
RN  - 0 (Plant Extracts)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/drug effects
MH  - Dendritic Cells/cytology/*drug effects/immunology
MH  - *Echinacea
MH  - Female
MH  - MAP Kinase Signaling System/drug effects
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - NF-kappa B/drug effects
MH  - Plant Extracts/*pharmacology
MH  - Signal Transduction/*drug effects/immunology
MH  - p38 Mitogen-Activated Protein Kinases/drug effects
OTO - NOTNLM
OT  - Dendritic cells
OT  - E. purpurea
OT  - MAPKs
OT  - Maturation
OT  - NF-kappaB
OT  - TLRs
EDAT- 2017/03/07 06:00
MHDA- 2018/02/10 06:00
CRDT- 2017/03/07 06:00
PHST- 2016/12/01 00:00 [received]
PHST- 2017/03/01 00:00 [revised]
PHST- 2017/03/01 00:00 [accepted]
PHST- 2017/03/07 06:00 [pubmed]
PHST- 2018/02/10 06:00 [medline]
PHST- 2017/03/07 06:00 [entrez]
AID - S0145-305X(16)30459-1 [pii]
AID - 10.1016/j.dci.2017.03.002 [doi]
PST - ppublish
SO  - Dev Comp Immunol. 2017 Aug;73:21-26. doi: 10.1016/j.dci.2017.03.002. Epub 2017 
      Mar 2.