PMID- 28264488
OWN - NLM
STAT- MEDLINE
DCOM- 20170817
LR  - 20181113
IS  - 2072-6643 (Electronic)
IS  - 2072-6643 (Linking)
VI  - 9
IP  - 3
DP  - 2017 Feb 28
TI  - Enantioselective Modulatory Effects of Naringenin  
      Enantiomers on the Expression Levels of miR-17-3p  
      Involved in Endogenous Antioxidant Defenses.
LID - 10.3390/nu9030215 [doi]
LID - 215
AB  - Naringenin is a flavanone present in citrus fruit as a mixture of chiral isomers. 
      The numerous biological properties attributed to this compound include 
      antioxidant and anti-inflammatory activities, even though the molecular 
      mechanisms of these remain unknown. This study aims to evaluate the effects of 
      racemic and enantiomeric naringenin on the expression levels of miR-17-3p, 
      miR-25-5p and relative mRNA targets, to elucidate the mechanisms underlying these 
      antioxidant and anti-inflammatory properties. Caco-2 cells, a well characterized 
      in vitro model which mimics the intestinal barrier, were treated with subtoxic 
      concentrations of racemate and enantiomers. The expression levels of miR-17-3p 
      and miR-25-5p were determined by Real-Time PCR and were found to be decreased for 
      both miRNAs. miR-17-3p behavior was in agreement with the increased levels of 
      target mRNAs coding for two antioxidant enzymes, manganese-dependent superoxide 
      dismutase (MnSOD) and glutathione peroxidase 2 (GPx2), while expression levels of 
      miR-25-5p were not in agreement with its target mRNAs, coding for two 
      pro-inflammatory cytokines, Tumor necrosis factor-alpha (TNF-alpha) and Interleukin-6 
      (IL-6). These results lead to the conclusion that naringenin could exert its 
      antioxidant activity through epigenetic regulation operated by miRNAs, while 
      anti-inflammatory activity is regulated by other miRNAs and/or mechanisms.
FAU - Curti, Valeria
AU  - Curti V
AD  - Department of Drug Sciences, Medicinal Chemistry and Pharmaceutical Technology 
      Section, Pavia University, Viale Taramelli 12, 27100 Pavia, Italy. 
      valeriacurti@kolinpharma.com.
AD  - KOLINPHARMA S.p.A., Lainate, Corso Europa 5, 20020 Lainate, Italy. 
      valeriacurti@kolinpharma.com.
FAU - Di Lorenzo, Arianna
AU  - Di Lorenzo A
AD  - Department of Drug Sciences, Medicinal Chemistry and Pharmaceutical Technology 
      Section, Pavia University, Viale Taramelli 12, 27100 Pavia, Italy. 
      arianna.dilorenzo01@universitadipavia.it.
AD  - KOLINPHARMA S.p.A., Lainate, Corso Europa 5, 20020 Lainate, Italy. 
      arianna.dilorenzo01@universitadipavia.it.
FAU - Rossi, Daniela
AU  - Rossi D
AD  - Department of Drug Sciences, Medicinal Chemistry and Pharmaceutical Technology 
      Section, Pavia University, Viale Taramelli 12, 27100 Pavia, Italy. 
      daniela.rossi@unipv.it.
FAU - Martino, Emanuela
AU  - Martino E
AD  - Department of Earth and Environmental Sciences, University of Pavia, Via S. 
      Epifanio 14, 27100 Pavia, Italy. emanuela.martino@unipv.it.
FAU - Capelli, Enrica
AU  - Capelli E
AD  - Department of Earth and Environmental Sciences, University of Pavia, Via S. 
      Epifanio 14, 27100 Pavia, Italy. enrica.capelli@unipv.it.
FAU - Collina, Simona
AU  - Collina S
AD  - Department of Drug Sciences, Medicinal Chemistry and Pharmaceutical Technology 
      Section, Pavia University, Viale Taramelli 12, 27100 Pavia, Italy. 
      simona.collina@unipv.it.
FAU - Daglia, Maria
AU  - Daglia M
AD  - Department of Drug Sciences, Medicinal Chemistry and Pharmaceutical Technology 
      Section, Pavia University, Viale Taramelli 12, 27100 Pavia, Italy. 
      maria.daglia@unipv.it.
LA  - eng
PT  - Journal Article
DEP - 20170228
PL  - Switzerland
TA  - Nutrients
JT  - Nutrients
JID - 101521595
RN  - 0 (Antioxidants)
RN  - 0 (Flavanones)
RN  - 0 (Interleukin-6)
RN  - 0 (MIRN17 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 1.11.1.- (GPX2 protein, human)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - HN5425SBF2 (naringenin)
SB  - IM
MH  - Antioxidants/*metabolism
MH  - Caco-2 Cells
MH  - Down-Regulation
MH  - Epigenesis, Genetic
MH  - Flavanones/*pharmacology
MH  - Glutathione Peroxidase/genetics/metabolism
MH  - Humans
MH  - Interleukin-6/genetics/metabolism
MH  - MicroRNAs/genetics/*metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Superoxide Dismutase/genetics/metabolism
MH  - Tumor Necrosis Factor-alpha/genetics/metabolism
MH  - Up-Regulation
PMC - PMC5372878
OTO - NOTNLM
OT  - naringenin racemate
OT  -  antioxidant enzymes
OT  -  caco-2 cells
OT  -  epigenetics
OT  -  microRNA
OT  -  naringenin enantiomers
OT  -  pro-inflammatory  cytokines
COIS- The authors have declared no conflict of interest.
EDAT- 2017/03/08 06:00
MHDA- 2017/08/18 06:00
PMCR- 2017/03/01
CRDT- 2017/03/08 06:00
PHST- 2016/11/28 00:00 [received]
PHST- 2017/02/24 00:00 [accepted]
PHST- 2017/03/08 06:00 [entrez]
PHST- 2017/03/08 06:00 [pubmed]
PHST- 2017/08/18 06:00 [medline]
PHST- 2017/03/01 00:00 [pmc-release]
AID - nu9030215 [pii]
AID - nutrients-09-00215 [pii]
AID - 10.3390/nu9030215 [doi]
PST - epublish
SO  - Nutrients. 2017 Feb 28;9(3):215. doi: 10.3390/nu9030215.