PMID- 28265582
OWN - NLM
STAT- MEDLINE
DCOM- 20170330
LR  - 20201209
IS  - 2314-7156 (Electronic)
IS  - 2314-8861 (Print)
IS  - 2314-7156 (Linking)
VI  - 2017
DP  - 2017
TI  - Glycomacropeptide Attenuates Inflammation, Pruritus, and Th2 Response Associated 
      with Atopic Dermatitis Induced by 2,4-Dinitrochlorobenzene in Rat.
PG  - 6935402
LID - 10.1155/2017/6935402 [doi]
LID - 6935402
AB  - Atopic dermatitis (AD) is one of the most common skin diseases, whose incidence 
      is increasing in industrialized countries. The epicutaneous application of a 
      hapten, such as 2,4-dinitrochlorobenzene (DNCB), evokes an experimental murine 
      AD-like reaction. Glycomacropeptide (GMP) is a dairy bioactive peptide derived 
      from hydrolysis of kappa-casein by chymosin action. It has anti-inflammatory, 
      prebiotic, and immunomodulatory effects. The present study was aimed to 
      investigate the effect of GMP administration on DNCB-induced AD in rats. The 
      severity of inflammatory process, pruritus, production of cytokines, and total 
      immunoglobulin E (IgE) content were measured, and the histopathological features 
      were analyzed. GMP reduced the intensity of inflammatory process and edema of 
      DNCB-induced dermatitis, with a significant decrease in eosinophils recruitment 
      and mast cells hyperplasia. In addition GMP suppressed the serum levels of total 
      IgE and IL-4, IL-5, and IL-13 expression in AD-lesions. Besides, the levels of 
      IL-10 were significantly increased. Remarkably, GMP administration before 
      AD-induction abolished pruritus in dermatitis-like reactions in the rats. Taken 
      together, these results indicate that GMP has an inhibitory effect on AD by 
      downregulating Th2 dominant immune response, suggesting GMP as a potential 
      effective alternative therapy for the prevention and management of AD.
FAU - Munoz, Fabiola Carolina
AU  - Munoz FC
AD  - Department of Microbiology, Basic Science Center, Autonomous University of 
      Aguascalientes, Av. Universidad No. 940, 20131 Aguascalientes, AGS, Mexico.
FAU - Cervantes, Maritza Montserrat
AU  - Cervantes MM
AUID- ORCID: 0000-0002-5535-3300
AD  - Department of Microbiology, Basic Science Center, Autonomous University of 
      Aguascalientes, Av. Universidad No. 940, 20131 Aguascalientes, AGS, Mexico.
FAU - Cervantes-Garcia, Daniel
AU  - Cervantes-Garcia D
AD  - Department of Microbiology, Basic Science Center, Autonomous University of 
      Aguascalientes, Av. Universidad No. 940, 20131 Aguascalientes, AGS, Mexico.
FAU - Jimenez, Mariela
AU  - Jimenez M
AD  - Department of Microbiology, Basic Science Center, Autonomous University of 
      Aguascalientes, Av. Universidad No. 940, 20131 Aguascalientes, AGS, Mexico.
FAU - Ventura-Juarez, Javier
AU  - Ventura-Juarez J
AD  - Department of Morphology, Basic Science Center, Autonomous University of 
      Aguascalientes, Av. Universidad No. 940, 20131 Aguascalientes, AGS, Mexico.
FAU - Salinas, Eva
AU  - Salinas E
AUID- ORCID: 0000-0002-3570-7255
AD  - Department of Microbiology, Basic Science Center, Autonomous University of 
      Aguascalientes, Av. Universidad No. 940, 20131 Aguascalientes, AGS, Mexico.
LA  - eng
PT  - Journal Article
DEP - 20170207
PL  - Egypt
TA  - J Immunol Res
JT  - Journal of immunology research
JID - 101627166
RN  - 0 (Anti-Inflammatory Agents, Non-Steroidal)
RN  - 0 (Caseins)
RN  - 0 (Dinitrochlorobenzene)
RN  - 0 (Interleukin-13)
RN  - 0 (Interleukin-5)
RN  - 0 (Peptide Fragments)
RN  - 0 (caseinomacropeptide)
RN  - 130068-27-8 (Interleukin-10)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents, Non-Steroidal/pharmacology/*therapeutic use
MH  - Caseins/*therapeutic use
MH  - Dermatitis, Atopic/chemically induced/*drug therapy/*immunology/physiopathology
MH  - Dinitrochlorobenzene
MH  - Disease Models, Animal
MH  - Eosinophils
MH  - Immunoglobulin E/blood
MH  - Inflammation/*drug therapy
MH  - Interleukin-10/blood/genetics
MH  - Interleukin-13/blood/genetics
MH  - Interleukin-4/blood/genetics
MH  - Interleukin-5/blood/genetics
MH  - Mast Cells
MH  - Peptide Fragments/*therapeutic use
MH  - Pruritus/*drug therapy
MH  - Rats
MH  - Skin/immunology/pathology
MH  - Th2 Cells/drug effects/*immunology
PMC - PMC5318634
COIS- The authors confirm that there are no competing interests.
EDAT- 2017/03/08 06:00
MHDA- 2017/03/31 06:00
PMCR- 2017/02/07
CRDT- 2017/03/08 06:00
PHST- 2016/12/06 00:00 [received]
PHST- 2017/01/12 00:00 [accepted]
PHST- 2017/03/08 06:00 [entrez]
PHST- 2017/03/08 06:00 [pubmed]
PHST- 2017/03/31 06:00 [medline]
PHST- 2017/02/07 00:00 [pmc-release]
AID - 10.1155/2017/6935402 [doi]
PST - ppublish
SO  - J Immunol Res. 2017;2017:6935402. doi: 10.1155/2017/6935402. Epub 2017 Feb 7.