PMID- 28266029
OWN - NLM
STAT- MEDLINE
DCOM- 20180223
LR  - 20181202
IS  - 2042-7158 (Electronic)
IS  - 0022-3573 (Linking)
VI  - 69
IP  - 6
DP  - 2017 Jun
TI  - Decorin-loaded poly lactic-co-glycolic acid nanoparticles modified by anti-alpha 
      fetoprotein antibody: preparation, proliferation inhibition and induced apoptosis 
      effects on HepG2 cells in vitro.
PG  - 633-641
LID - 10.1111/jphp.12695 [doi]
AB  - OBJECTIVES: Decorin (DCN) is a negative regulatory factor for the growth of 
      cancer cells and can inhibit the proliferation, metastasis of cancer cells and 
      angiogenesis in cancer tissues. The aims of this study were to prepare the 
      nanoparticles consisting of DCN and poly lactic-co-glycolic acid (PLGA) modified 
      by anti-alpha fetoprotein (AFP) monoclonal antibody (mAb) and to examine the 
      conventional physical properties, the in-vitro release of DCN and the targeting 
      effect of these nanoparticles on HepG2 cells. KEY FINDINGS: The encapsulated 
      plasmid was slowly and steadily released from the nanoparticles. The targeted 
      PLGA nanoparticles were initiatively taken in HepG2 cells high-efficiently. 
      According to the results of RT-PCR, DCN gene in AFPmAb-PLGA-rhDCN nanoparticles 
      can be expressed in HepG2 cells successfully. These nanoparticles significantly 
      inhibited the proliferation of HepG2 cells and induced apoptosis. The mRNA 
      expression of Bcl-2 gene in the AFPmAb-PLGA-rhDCN-treated groups appeared 
      significantly to decrease and the caspase-3 gene had the opposite trend as 
      compared with that of control group (P < 0.01). CONCLUSION: These studies 
      revealed that these nanoparticles were capable of specifically targeting the 
      HepG2 cells and inhibiting the proliferation and they induce apoptosis of HepG2 
      cells in vitro, which was in a dose- and time-dependent manner.
CI  - (c) 2017 Royal Pharmaceutical Society.
FAU - Yang, Qiaoli
AU  - Yang Q
AD  - Department of Immunology and Microbiology, Shanxi Medical University, Taiyuan, 
      China.
FAU - Wang, Shuyue
AU  - Wang S
AD  - Department of Immunology and Microbiology, Shanxi Medical University, Taiyuan, 
      China.
FAU - Wang, Yuan
AU  - Wang Y
AD  - Department of Immunology and Microbiology, Shanxi Medical University, Taiyuan, 
      China.
FAU - Qu, Yane
AU  - Qu Y
AD  - Department of Immunology and Microbiology, Shanxi Medical University, Taiyuan, 
      China.
FAU - Xue, Jun
AU  - Xue J
AD  - Department of Immunology and Microbiology, Shanxi Medical University, Taiyuan, 
      China.
FAU - Mi, Yang
AU  - Mi Y
AD  - Department of Immunology and Microbiology, Shanxi Medical University, Taiyuan, 
      China.
FAU - Wang, Yanhong
AU  - Wang Y
AD  - Department of Immunology and Microbiology, Shanxi Medical University, Taiyuan, 
      China.
FAU - Luo, Xuguang
AU  - Luo X
AD  - Department of Immunology and Microbiology, Shanxi Medical University, Taiyuan, 
      China.
FAU - Deng, Zhihua
AU  - Deng Z
AD  - Second Clinical College of Shanxi Medical University, Taiyuan, China.
FAU - Wang, Guiqin
AU  - Wang G
AD  - Department of Immunology and Microbiology, Shanxi Medical University, Taiyuan, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20170307
PL  - England
TA  - J Pharm Pharmacol
JT  - The Journal of pharmacy and pharmacology
JID - 0376363
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Decorin)
RN  - 0 (RNA, Messenger)
RN  - 0 (alpha-Fetoproteins)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Antibodies, Monoclonal/chemistry/*pharmacology
MH  - Antineoplastic Agents/chemistry/pharmacology
MH  - Apoptosis/*drug effects
MH  - Caspase 3/metabolism
MH  - Cell Line, Tumor
MH  - Cell Proliferation/*drug effects
MH  - Decorin/*chemistry
MH  - Genes, bcl-2/genetics
MH  - Hep G2 Cells
MH  - Humans
MH  - Lactic Acid/*chemistry
MH  - Nanoparticles/*chemistry
MH  - Polyglycolic Acid/*chemistry
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - RNA, Messenger/genetics
MH  - alpha-Fetoproteins/chemistry/*pharmacology
OTO - NOTNLM
OT  - HepG2 cell
OT  - alpha fetoprotein
OT  - decorin
OT  - monoclonal antibody
OT  - poly lactic-co-glycolic acid
EDAT- 2017/03/08 06:00
MHDA- 2018/02/24 06:00
CRDT- 2017/03/08 06:00
PHST- 2016/08/02 00:00 [received]
PHST- 2016/12/11 00:00 [accepted]
PHST- 2017/03/08 06:00 [pubmed]
PHST- 2018/02/24 06:00 [medline]
PHST- 2017/03/08 06:00 [entrez]
AID - 10.1111/jphp.12695 [doi]
PST - ppublish
SO  - J Pharm Pharmacol. 2017 Jun;69(6):633-641. doi: 10.1111/jphp.12695. Epub 2017 Mar 
      7.