PMID- 2826631
OWN - NLM
STAT- MEDLINE
DCOM- 19880225
LR  - 20190516
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 43
IP  - 2
DP  - 1988 Feb
TI  - Mannose inhibits the human neutrophil oxidative burst.
PG  - 158-64
AB  - Stimulated human neutrophils (PMNs) increase their oxygen consumption and secrete 
      reactive oxygen species that are involved in bactericidal activity and 
      inflammation. While studying lectin-mediated bacterial adherence, we observed 
      that D-mannose appeared to inhibit PMN metabolism. Further studies showed that 
      100 mM mannose inhibited oxygen consumption by 82%, superoxide secretion by 84%, 
      luminol-enhanced chemiluminescence (CL) by 98%, and hexose-monophosphate shunt 
      activity by 100% when PMN were stimulated with 1 microM phorbol myristate acetate 
      (PMA). Inhibition was also seen with 0.1 microM 
      formyl-methionyl-leucyl-phenylalanine (fMLP), and 0.1 microM A23187, reagents 
      thought to stimulate the respiratory burst by different transductional 
      mechanisms. Inhibition was dose-responsive and specific since 100 mM D-galactose, 
      alpha-D-glucose, or alpha-L-fucose only minimally affected PMN oxidative 
      metabolism. Inhibition of PMA-induced superoxide production was seen almost 
      immediately upon the addition of 50 mM mannose and was reversed by washing. 
      Neutrophils remained viable as measured by trypan blue exclusion. These data 
      suggest that mannose inhibits the neutrophil oxidative burst at the level of the 
      hexose monophosphate shunt. Further investigation should elucidate the specific 
      mechanism(s) of this burst inhibition as well as define uses for it as a tool to 
      study oxidative as well as nonoxidative killing by PMN.
FAU - Rest, R F
AU  - Rest RF
AD  - Department of Microbiology and Immunology, Hahnemann University School of 
      Medicine, Philadelphia, PA 19102-1192.
FAU - Farrell, C F
AU  - Farrell CF
FAU - Naids, F L
AU  - Naids FL
LA  - eng
GR  - AI20897/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 11062-77-4 (Superoxides)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
RN  - PHA4727WTP (Mannose)
SB  - IM
MH  - Humans
MH  - Luminescent Measurements
MH  - Mannose/*pharmacology
MH  - Neutrophils/*metabolism
MH  - Oxygen Consumption/*drug effects
MH  - Superoxides/*metabolism
MH  - Tetradecanoylphorbol Acetate/pharmacology
EDAT- 1988/02/01 00:00
MHDA- 1988/02/01 00:01
CRDT- 1988/02/01 00:00
PHST- 1988/02/01 00:00 [pubmed]
PHST- 1988/02/01 00:01 [medline]
PHST- 1988/02/01 00:00 [entrez]
AID - 10.1002/jlb.43.2.158 [doi]
PST - ppublish
SO  - J Leukoc Biol. 1988 Feb;43(2):158-64. doi: 10.1002/jlb.43.2.158.