PMID- 28270601
OWN - NLM
STAT- MEDLINE
DCOM- 20180423
LR  - 20210904
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 114
IP  - 12
DP  - 2017 Mar 21
TI  - Resuscitation of Pseudomonas aeruginosa from dormancy requires hibernation 
      promoting factor (PA4463) for ribosome preservation.
PG  - 3204-3209
LID - 10.1073/pnas.1700695114 [doi]
AB  - Pseudomonas aeruginosa biofilm infections are difficult to treat with antibiotic 
      therapy in part because the biofilms contain subpopulations of dormant 
      antibiotic-tolerant cells. The dormant cells can repopulate the biofilms 
      following alleviation of antibiotic treatments. While dormant, the bacteria must 
      maintain cellular integrity, including ribosome abundance, to reinitiate the de 
      novo protein synthesis required for resuscitation. Here, we demonstrate that the 
      P. aeruginosa gene PA4463 [hibernation promoting factor (HPF)], but not the 
      ribosome modulation factor (PA3049), is required for ribosomal RNA preservation 
      during prolonged nutrient starvation conditions. Single-cell-level studies using 
      fluorescence in situ hybridization (FISH) and growth in microfluidic drops 
      demonstrate that, in the absence of hpf, the rRNA abundances of starved cells 
      decrease to levels that cause them to lose their ability to resuscitate from 
      starvation, leaving intact nondividing cells. P. aeruginosa defective in the 
      stringent response also had reduced ability to resuscitate from dormancy. 
      However, FISH analysis of the starved stringent response mutant showed a bimodal 
      response where the individual cells contained either abundant or low ribosome 
      content, compared with the wild-type strain. The results indicate that ribosome 
      maintenance is key for maintaining the ability of P. aeruginosa to resuscitate 
      from starvation-induced dormancy and that HPF is the major factor associated with 
      P. aeruginosa ribosome preservation.
FAU - Akiyama, Tatsuya
AU  - Akiyama T
AD  - Department of Microbiology and Immunology, Montana State University, Bozeman, MT 
      59717.
AD  - Center for Biofilm Engineering, Montana State University, Bozeman, MT 59717.
FAU - Williamson, Kerry S
AU  - Williamson KS
AD  - Department of Microbiology and Immunology, Montana State University, Bozeman, MT 
      59717.
AD  - Center for Biofilm Engineering, Montana State University, Bozeman, MT 59717.
FAU - Schaefer, Robert
AU  - Schaefer R
AD  - Center for Biofilm Engineering, Montana State University, Bozeman, MT 59717.
AD  - Department of Chemical and Biological Engineering, Montana State University, 
      Bozeman, MT 59717.
FAU - Pratt, Shawna
AU  - Pratt S
AD  - Center for Biofilm Engineering, Montana State University, Bozeman, MT 59717.
AD  - Department of Chemical and Biological Engineering, Montana State University, 
      Bozeman, MT 59717.
FAU - Chang, Connie B
AU  - Chang CB
AD  - Center for Biofilm Engineering, Montana State University, Bozeman, MT 59717.
AD  - Department of Chemical and Biological Engineering, Montana State University, 
      Bozeman, MT 59717.
FAU - Franklin, Michael J
AU  - Franklin MJ
AD  - Department of Microbiology and Immunology, Montana State University, Bozeman, MT 
      59717; franklin@montana.edu.
AD  - Center for Biofilm Engineering, Montana State University, Bozeman, MT 59717.
LA  - eng
GR  - P20 GM103474/GM/NIGMS NIH HHS/United States
GR  - R01 AI113330/AI/NIAID NIH HHS/United States
GR  - R21 AI140163/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20170307
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (RNA, Ribosomal)
RN  - 0 (Ribosomal Proteins)
SB  - IM
MH  - Energy Metabolism
MH  - Gene Expression Regulation, Bacterial
MH  - *Hibernation
MH  - Pseudomonas aeruginosa/*physiology
MH  - RNA, Ribosomal/genetics/metabolism
MH  - Ribosomal Proteins/*metabolism
MH  - Ribosomes/*metabolism
PMC - PMC5373351
OTO - NOTNLM
OT  - dormancy
OT  - hibernation promoting factor
OT  - rRNA
OT  - resuscitation
OT  - ribosome
COIS- The authors declare no conflict of interest.
EDAT- 2017/03/09 06:00
MHDA- 2018/04/24 06:00
PMCR- 2017/09/21
CRDT- 2017/03/09 06:00
PHST- 2017/03/09 06:00 [pubmed]
PHST- 2018/04/24 06:00 [medline]
PHST- 2017/03/09 06:00 [entrez]
PHST- 2017/09/21 00:00 [pmc-release]
AID - 1700695114 [pii]
AID - 201700695 [pii]
AID - 10.1073/pnas.1700695114 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2017 Mar 21;114(12):3204-3209. doi: 
      10.1073/pnas.1700695114. Epub 2017 Mar 7.