PMID- 28275300
OWN - NLM
STAT- MEDLINE
DCOM- 20170814
LR  - 20181113
IS  - 2219-2840 (Electronic)
IS  - 1007-9327 (Print)
IS  - 1007-9327 (Linking)
VI  - 23
IP  - 7
DP  - 2017 Feb 21
TI  - Caffeic acid phenethyl ester up-regulates antioxidant levels in hepatic stellate 
      cell line T6 via an Nrf2-mediated mitogen activated protein kinases pathway.
PG  - 1203-1214
LID - 10.3748/wjg.v23.i7.1203 [doi]
AB  - AIM: To investigate the antioxidant effect of caffeic acid phenethyl ester (CAPE) 
      in hepatic stellate cell-T6 (HSC-T6) cells cultured in vitro and the potential 
      mechanisms. METHODS: HSC-T6 cells were cultured in vitro and treated with various 
      concentrations of CAPE for 24, 48 and 72 h, respectively. Cell proliferation was 
      investigated using the MTT assay, and cell ultrastructural alterations were 
      observed by transmission electron microscopy. Flow cytometry was employed to 
      investigate the effects of CAPE on apoptosis and the levels of reactive oxygen 
      species in HSC-T6 cells cultured in vitro. An enzyme immunoassay instrument was 
      used to evaluate antioxidant enzyme expression. The effect on alpha-smooth muscle 
      actin was shown using immunofluorescence. Gene and protein levels of Nrf2, 
      related factors, and mitogen activated protein kinases (MAPKs), in HSC-T6 cells 
      were detected using RT-PCR and Western blot, respectively. RESULTS: CAPE 
      inhibited the proliferation and activation of HSC-T6 cells cultured in vitro. 
      CAPE increased the antioxidant levels and the translocation of Nrf2 from the 
      cytoplasm to the nucleus in HSC-T6 cells. Moreover, the phosphorylation of MAPKs 
      in cells decreased in response to CAPE. Interestingly, CAPE-induced oxidative 
      stress in the cells was significantly attenuated by pretreatment with MAPKs 
      inhibitors. CONCLUSION: CAPE inhibits cell proliferation and up-regulates the 
      antioxidant levels in HSC-T6 cells partly through the Nrf2-MAPKs signaling 
      pathway.
FAU - Yang, Ning
AU  - Yang N
AD  - Ning Yang, Juan-Juan Shi, Feng-Ping Wu, Mei Li, Xin Zhang, Ya-Ping Li, Song Zhai, 
      Xiao-Li Jia, Shuang-Suo Dang, Department of Infectious Diseases, the Second 
      Affiliated Hospital of Medical School of Xi'an Jiaotong University, Xi'an 710004, 
      Shannxi Province, China.
FAU - Shi, Juan-Juan
AU  - Shi JJ
AD  - Ning Yang, Juan-Juan Shi, Feng-Ping Wu, Mei Li, Xin Zhang, Ya-Ping Li, Song Zhai, 
      Xiao-Li Jia, Shuang-Suo Dang, Department of Infectious Diseases, the Second 
      Affiliated Hospital of Medical School of Xi'an Jiaotong University, Xi'an 710004, 
      Shannxi Province, China.
FAU - Wu, Feng-Ping
AU  - Wu FP
AD  - Ning Yang, Juan-Juan Shi, Feng-Ping Wu, Mei Li, Xin Zhang, Ya-Ping Li, Song Zhai, 
      Xiao-Li Jia, Shuang-Suo Dang, Department of Infectious Diseases, the Second 
      Affiliated Hospital of Medical School of Xi'an Jiaotong University, Xi'an 710004, 
      Shannxi Province, China.
FAU - Li, Mei
AU  - Li M
AD  - Ning Yang, Juan-Juan Shi, Feng-Ping Wu, Mei Li, Xin Zhang, Ya-Ping Li, Song Zhai, 
      Xiao-Li Jia, Shuang-Suo Dang, Department of Infectious Diseases, the Second 
      Affiliated Hospital of Medical School of Xi'an Jiaotong University, Xi'an 710004, 
      Shannxi Province, China.
FAU - Zhang, Xin
AU  - Zhang X
AD  - Ning Yang, Juan-Juan Shi, Feng-Ping Wu, Mei Li, Xin Zhang, Ya-Ping Li, Song Zhai, 
      Xiao-Li Jia, Shuang-Suo Dang, Department of Infectious Diseases, the Second 
      Affiliated Hospital of Medical School of Xi'an Jiaotong University, Xi'an 710004, 
      Shannxi Province, China.
FAU - Li, Ya-Ping
AU  - Li YP
AD  - Ning Yang, Juan-Juan Shi, Feng-Ping Wu, Mei Li, Xin Zhang, Ya-Ping Li, Song Zhai, 
      Xiao-Li Jia, Shuang-Suo Dang, Department of Infectious Diseases, the Second 
      Affiliated Hospital of Medical School of Xi'an Jiaotong University, Xi'an 710004, 
      Shannxi Province, China.
FAU - Zhai, Song
AU  - Zhai S
AD  - Ning Yang, Juan-Juan Shi, Feng-Ping Wu, Mei Li, Xin Zhang, Ya-Ping Li, Song Zhai, 
      Xiao-Li Jia, Shuang-Suo Dang, Department of Infectious Diseases, the Second 
      Affiliated Hospital of Medical School of Xi'an Jiaotong University, Xi'an 710004, 
      Shannxi Province, China.
FAU - Jia, Xiao-Li
AU  - Jia XL
AD  - Ning Yang, Juan-Juan Shi, Feng-Ping Wu, Mei Li, Xin Zhang, Ya-Ping Li, Song Zhai, 
      Xiao-Li Jia, Shuang-Suo Dang, Department of Infectious Diseases, the Second 
      Affiliated Hospital of Medical School of Xi'an Jiaotong University, Xi'an 710004, 
      Shannxi Province, China.
FAU - Dang, Shuang-Suo
AU  - Dang SS
AD  - Ning Yang, Juan-Juan Shi, Feng-Ping Wu, Mei Li, Xin Zhang, Ya-Ping Li, Song Zhai, 
      Xiao-Li Jia, Shuang-Suo Dang, Department of Infectious Diseases, the Second 
      Affiliated Hospital of Medical School of Xi'an Jiaotong University, Xi'an 710004, 
      Shannxi Province, China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - World J Gastroenterol
JT  - World journal of gastroenterology
JID - 100883448
RN  - 0 (Antioxidants)
RN  - 0 (Caffeic Acids)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (Nfe2l2 protein, rat)
RN  - 0 (Reactive Oxygen Species)
RN  - 339NCG44TV (Phenol)
RN  - G960R9S5SK (caffeic acid phenethyl ester)
RN  - ML9LGA7468 (Phenylethyl Alcohol)
SB  - IM
MH  - Animals
MH  - Antioxidants/*chemistry
MH  - Apoptosis
MH  - Caffeic Acids/*therapeutic use
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Flow Cytometry
MH  - Hepatic Stellate Cells/*drug effects/metabolism
MH  - Immunoenzyme Techniques
MH  - *MAP Kinase Signaling System
MH  - Microscopy, Fluorescence
MH  - NF-E2-Related Factor 2/*metabolism
MH  - Oxidative Stress
MH  - Phenol
MH  - Phenylethyl Alcohol/*analogs & derivatives/therapeutic use
MH  - Protein Transport
MH  - Rats
MH  - Reactive Oxygen Species/metabolism
PMC - PMC5323445
OTO - NOTNLM
OT  - Antioxidation
OT  - Caffeic acid phenethyl ester
OT  - Liver fibrosis
OT  - Mitogen activated protein kinases
OT  - Nrf2
COIS- Conflict-of-interest statement: To the best of our knowledge, no conflict of 
      interest exists.
EDAT- 2017/03/10 06:00
MHDA- 2017/08/15 06:00
PMCR- 2017/02/21
CRDT- 2017/03/10 06:00
PHST- 2016/11/15 00:00 [received]
PHST- 2016/12/27 00:00 [revised]
PHST- 2017/01/11 00:00 [accepted]
PHST- 2017/03/10 06:00 [entrez]
PHST- 2017/03/10 06:00 [pubmed]
PHST- 2017/08/15 06:00 [medline]
PHST- 2017/02/21 00:00 [pmc-release]
AID - 10.3748/wjg.v23.i7.1203 [doi]
PST - ppublish
SO  - World J Gastroenterol. 2017 Feb 21;23(7):1203-1214. doi: 10.3748/wjg.v23.i7.1203.