PMID- 28275945
OWN - NLM
STAT- MEDLINE
DCOM- 20180619
LR  - 20181113
IS  - 1874-9356 (Electronic)
IS  - 0015-5632 (Print)
IS  - 0015-5632 (Linking)
VI  - 62
IP  - 5
DP  - 2017 Sep
TI  - Enrichment culture and identification of endophytic methanotrophs isolated from 
      peatland plants.
PG  - 381-391
LID - 10.1007/s12223-017-0508-9 [doi]
AB  - Aerobic methane-oxidizing bacteria (MOB) are an environmentally significant group 
      of microorganisms due to their role in the global carbon cycle. Research 
      conducted over the past few decades has increased the interest in discovering 
      novel genera of methane-degrading bacteria, which efficiently utilize methane and 
      decrease the global warming effect. Moreover, methanotrophs have more promising 
      applications in environmental bioengineering, biotechnology, and pharmacy. The 
      investigations were undertaken to recognize the variety of endophytic 
      methanotrophic bacteria associated with Carex nigra, Vaccinium oxycoccus, and 
      Eriophorum vaginatum originating from Moszne peatland (East Poland). 
      Methanotrophic bacteria were isolated from plants by adding sterile fragments of 
      different parts of plants (roots and stems) to agar mineral medium (nitrate 
      mineral salts (NMS)) and incubated at different methane values (1-20% CH4). 
      Single colonies were streaked on new NMS agar media and, after incubation, 
      transferred to liquid NMS medium. Bacterial growth dynamics in the culture 
      solution was studied by optical density-OD600 and methane consumption. Changes in 
      the methane concentration during incubation were controlled by the gas 
      chromatography technique. Characterization of methanotrophs was made by 
      fluorescence in situ hybridization (FISH) with Mg705 and Mg84 for type I 
      methanotrophs and Ma450 for type II methanotrophs. Identification of endophytes 
      was performed after 16S ribosomal RNA (rRNA) and mmoX gene amplification. Our 
      study confirmed the presence of both types of methanotrophic bacteria (types I 
      and II) with the predominance of type I methanotrophs. Among cultivable 
      methanotrophs, there were different strains of the genus Methylomonas and 
      Methylosinus. Furthermore, we determined the potential of the examined bacteria 
      for methane oxidation, which ranged from 0.463 +/- 0.067 to 5.928 +/- 0.169 mumol/L 
      CH4/mL/day.
FAU - Stepniewska, Zofia
AU  - Stepniewska Z
AD  - Department of Biochemistry and Environmental Chemistry, Institute of 
      Biotechnology, The John Paul II Catholic University of Lublin, Konstantynow 1I, 
      20-708, Lublin, Poland.
FAU - Goraj, Weronika
AU  - Goraj W
AD  - Department of Biochemistry and Environmental Chemistry, Institute of 
      Biotechnology, The John Paul II Catholic University of Lublin, Konstantynow 1I, 
      20-708, Lublin, Poland. weronikagoraj@kul.pl.
FAU - Kuzniar, Agnieszka
AU  - Kuzniar A
AD  - Department of Biochemistry and Environmental Chemistry, Institute of 
      Biotechnology, The John Paul II Catholic University of Lublin, Konstantynow 1I, 
      20-708, Lublin, Poland.
FAU - Lopacka, Natalia
AU  - Lopacka N
AD  - Department of Biochemistry and Environmental Chemistry, Institute of 
      Biotechnology, The John Paul II Catholic University of Lublin, Konstantynow 1I, 
      20-708, Lublin, Poland.
FAU - Malysza, Magdalena
AU  - Malysza M
AD  - Department of Biochemistry and Environmental Chemistry, Institute of 
      Biotechnology, The John Paul II Catholic University of Lublin, Konstantynow 1I, 
      20-708, Lublin, Poland.
LA  - eng
PT  - Journal Article
DEP - 20170309
PL  - United States
TA  - Folia Microbiol (Praha)
JT  - Folia microbiologica
JID - 0376757
RN  - 0 (Culture Media)
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - OP0UW79H66 (Methane)
SB  - IM
MH  - Bacteriological Techniques
MH  - Chromatography, Gas
MH  - Culture Media/chemistry
MH  - Cyperaceae/*microbiology
MH  - DNA, Bacterial/chemistry/genetics
MH  - DNA, Ribosomal/chemistry/genetics
MH  - Endophytes/classification/growth & development/*isolation & 
      purification/metabolism
MH  - In Situ Hybridization, Fluorescence
MH  - Methane/*metabolism
MH  - Methylomonas/classification/growth & development/*isolation & 
      purification/metabolism
MH  - Methylosinus/classification/growth & development/*isolation & 
      purification/metabolism
MH  - Poland
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - Vaccinium/*microbiology
PMC - PMC5579069
EDAT- 2017/03/10 06:00
MHDA- 2018/06/21 06:00
PMCR- 2017/03/09
CRDT- 2017/03/10 06:00
PHST- 2016/06/28 00:00 [received]
PHST- 2017/02/16 00:00 [accepted]
PHST- 2017/03/10 06:00 [pubmed]
PHST- 2018/06/21 06:00 [medline]
PHST- 2017/03/10 06:00 [entrez]
PHST- 2017/03/09 00:00 [pmc-release]
AID - 10.1007/s12223-017-0508-9 [pii]
AID - 508 [pii]
AID - 10.1007/s12223-017-0508-9 [doi]
PST - ppublish
SO  - Folia Microbiol (Praha). 2017 Sep;62(5):381-391. doi: 10.1007/s12223-017-0508-9. 
      Epub 2017 Mar 9.