PMID- 28275994 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20200929 IS - 2191-0855 (Print) IS - 2191-0855 (Electronic) IS - 2191-0855 (Linking) VI - 7 IP - 1 DP - 2017 Dec TI - Experimental and visual research on the microbial induced carbonate precipitation by Pseudomonas aeruginosa. PG - 57 LID - 10.1186/s13568-017-0358-5 [doi] LID - 57 AB - Microbial induced carbonate precipitation (MICP) is a common occurrence of geochemistry influences in many fields, such as biological, geographical, and engineering systems. However, the processes that control interactions between carbonate biomineralization and biofilm properties are poorly understood. We develop a method for real time, in situ and nondestructive imaging with confocal scanning microscopy. This method provides a possible way to observe biomineralization process and the morphology of biomineralized deposits within biofilms. We use this method to show calcite biominerals produced by Pseudomonas aeruginosa biofilms which extremely change biofilm structures. The distribution of calcite precipitation produced in situ biomineralization is highly heterogeneous in biofilms and also to occur primarily on the bottom of biofilms. It is distinct from those usual expectations that mineral started to precipitate from surface of biofilm. Our results reveal that biomineralization plays a comprehensive regulation function on biofilm architecture and properties. FAU - Bai, Yang AU - Bai Y AD - College of Geosciences and Environmental Engineering, Southwest Jiaotong University, X4434, Chengdu, 611756, Sichuan, China. FAU - Guo, Xu-Jing AU - Guo XJ AD - College of Resources and Environment, Chengdu University of Information Technology, Chengdu, 610225, China. FAU - Li, Yun-Zhen AU - Li YZ AD - Sichuan Academy of Environmental Science, Chengdu, 610041, China. FAU - Huang, Tao AU - Huang T AD - College of Geosciences and Environmental Engineering, Southwest Jiaotong University, X4434, Chengdu, 611756, Sichuan, China. taohuangacd@126.com. LA - eng PT - Journal Article DEP - 20170309 PL - Germany TA - AMB Express JT - AMB Express JID - 101561785 PMC - PMC5342990 OTO - NOTNLM OT - Biofilms OT - Biomineralization OT - Calcium carbonate OT - MICP EDAT- 2017/03/10 06:00 MHDA- 2017/03/10 06:01 PMCR- 2017/03/09 CRDT- 2017/03/10 06:00 PHST- 2016/12/21 00:00 [received] PHST- 2017/03/02 00:00 [accepted] PHST- 2017/03/10 06:00 [entrez] PHST- 2017/03/10 06:00 [pubmed] PHST- 2017/03/10 06:01 [medline] PHST- 2017/03/09 00:00 [pmc-release] AID - 10.1186/s13568-017-0358-5 [pii] AID - 358 [pii] AID - 10.1186/s13568-017-0358-5 [doi] PST - ppublish SO - AMB Express. 2017 Dec;7(1):57. doi: 10.1186/s13568-017-0358-5. Epub 2017 Mar 9.