PMID- 28276540
OWN - NLM
STAT- MEDLINE
DCOM- 20180326
LR  - 20180326
IS  - 2047-4849 (Electronic)
IS  - 2047-4830 (Linking)
VI  - 5
IP  - 4
DP  - 2017 Mar 28
TI  - Rapid fabrication of functionalised poly(dimethylsiloxane) microwells for cell 
      aggregate formation.
PG  - 828-836
LID - 10.1039/c6bm00916f [doi]
AB  - Cell aggregates reproduce many features of the natural architecture of functional 
      tissues, and have therefore become an important in vitro model of tissue 
      function. In this study, we present an efficient and rapid method for the 
      fabrication of site specific functionalised poly(dimethylsiloxane) (PDMS) 
      microwell arrays that promote the formation of insulin-producing beta cell (MIN6) 
      aggregates. Microwells were prepared using an ice templating technique whereby 
      aqueous droplets were frozen on a surface and PDMS was cast on top to form a 
      replica. By employing an aqueous alkali hydroxide solution, we demonstrate 
      exclusive etching and functionalisation of the microwell inner surface, thereby 
      allowing the selective absorption of biological factors within the microwells. 
      Additionally, by manipulating surface wettability of the substrate through plasma 
      polymer coating, the shape and profile of the microwells could be tailored. 
      Microwells coated with antifouling Pluronic 123, bovine serum albumin, collagen 
      type IV or insulin growth factor 2 were employed to investigate the formation and 
      stability of MIN6 aggregates in microwells of different shapes. MIN6 aggregates 
      formed with this technique retained insulin expression. These results demonstrate 
      the potential of this platform for the rapid screening of biological factors 
      influencing the formation and response of insulin-producing cell aggregates 
      without the need for expensive micromachining techniques.
FAU - Forget, A
AU  - Forget A
AUID- ORCID: 0000-0002-3305-3980
AD  - Science and Engineering Faculty, Queensland University of Technology, Brisbane, 
      QLD 4001, Australia and Cooperative Research Centre for Cell Therapy 
      Manufacturing (CRC-CTM), Adelaide 5000, Australia.
FAU - Burzava, A L S
AU  - Burzava ALS
AD  - Cooperative Research Centre for Cell Therapy Manufacturing (CRC-CTM), Adelaide 
      5000, Australia and Monash Institute of Pharmaceutical Sciences, Monash 
      University, Parkville, VIC 3052, Australia. fnicolas.voelcker@monash.edu.
FAU - Delalat, B
AU  - Delalat B
AD  - Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, VIC 
      3052, Australia. fnicolas.voelcker@monash.edu.
FAU - Vasilev, K
AU  - Vasilev K
AD  - Cooperative Research Centre for Cell Therapy Manufacturing (CRC-CTM), Adelaide 
      5000, Australia and School of Engineering, University of South Australia, Mawson 
      Lakes 5095, Australia and Future Industries Institute, University of South 
      Australia, Mawson Lakes 5095, Australia.
FAU - Harding, F J
AU  - Harding FJ
AD  - Cooperative Research Centre for Cell Therapy Manufacturing (CRC-CTM), Adelaide 
      5000, Australia and Future Industries Institute, University of South Australia, 
      Mawson Lakes 5095, Australia and Cell Therapies Pty Ltd, Victorian Comprehensive 
      Cancer Centre (VCCC), Melbourne 3000, Australia. 
      franharding@celltherapies.com.au.
FAU - Blencowe, A
AU  - Blencowe A
AD  - Cooperative Research Centre for Cell Therapy Manufacturing (CRC-CTM), Adelaide 
      5000, Australia and Future Industries Institute, University of South Australia, 
      Mawson Lakes 5095, Australia and Cell Therapies Pty Ltd, Victorian Comprehensive 
      Cancer Centre (VCCC), Melbourne 3000, Australia. franharding@celltherapies.com.au 
      and School of Pharmacy and Medical Science, University of South Australia, 
      Adelaide 5000, Australia. anton.blencowe@unisa.edu.au.
FAU - Voelcker, N H
AU  - Voelcker NH
AUID- ORCID: 0000-0002-1536-7804
AD  - Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, VIC 
      3052, Australia. fnicolas.voelcker@monash.edu.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Biomater Sci
JT  - Biomaterials science
JID - 101593571
RN  - 0 (Biocompatible Materials)
RN  - 0 (Dimethylpolysiloxanes)
RN  - 0 (Immobilized Proteins)
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - 63148-62-9 (baysilon)
SB  - IM
EIN - Biomater Sci. 2017 May 2;5(5):1061. PMID: 28406497
MH  - Animals
MH  - Biocompatible Materials/*chemistry
MH  - Cattle
MH  - Cell Aggregation
MH  - Cell Culture Techniques/*instrumentation/methods
MH  - Cell Line
MH  - Dimethylpolysiloxanes/*chemistry
MH  - Equipment Design
MH  - Immobilized Proteins/chemistry
MH  - Insulin-Secreting Cells/*cytology
MH  - Mice
MH  - Serum Albumin, Bovine/chemistry
MH  - Spheroids, Cellular
MH  - Wettability
EDAT- 2017/03/10 06:00
MHDA- 2018/03/27 06:00
CRDT- 2017/03/10 06:00
PHST- 2017/03/10 06:00 [pubmed]
PHST- 2018/03/27 06:00 [medline]
PHST- 2017/03/10 06:00 [entrez]
AID - 10.1039/c6bm00916f [doi]
PST - ppublish
SO  - Biomater Sci. 2017 Mar 28;5(4):828-836. doi: 10.1039/c6bm00916f.