PMID- 28279229
OWN - NLM
STAT- MEDLINE
DCOM- 20171120
LR  - 20181202
IS  - 1757-6512 (Electronic)
IS  - 1757-6512 (Linking)
VI  - 8
IP  - 1
DP  - 2017 Mar 9
TI  - Human umbilical cord mesenchymal stem cells improve the reserve function of 
      perimenopausal ovary via a paracrine mechanism.
PG  - 55
LID - 10.1186/s13287-017-0514-5 [doi]
LID - 55
AB  - BACKGROUND: Human umbilical cord mesenchymal stem cells (hUCMSCs) are a type of 
      pluripotent stem cell which are isolated from the umbilical cord of newborns. 
      hUCMSCs have great therapeutic potential. We designed this experimental study in 
      order to investigate whether the transplantation of hUCMSCs can improve the 
      ovarian reserve function of perimenopausal rats and delay ovarian senescence. 
      METHOD: We selected naturally aging rats confirmed by vaginal smears as models of 
      perimenopausal rats, divided into the control group and the treatment group, and 
      selected young fertile female rats as normal controls. hUCMSCs were transplanted 
      into rats of the treatment group through tail veins. Enzyme-linked immunosorbent 
      assay (ELISA) detected serum levels of sex hormones, H&E staining showed ovarian 
      tissue structure and allowed follicle counting, immunohistochemistry and western 
      blot analysis revealed ovarian expression of hepatocyte growth factor (HGF), 
      vascular endothelial cell growth factor (VEGF), and insulin-like growth factor-1 
      (IGF-1), polymerase chain reaction (PCR) and western blot analysis revealed 
      hUCMSCs expression of HGF, VEGF, and IGF-1. RESULTS: At time points of 14, 21, 
      and 28 days after hUCMSCs transplantation, estradiol (E(2)) and anti-Mullerian 
      hormone (AMH) increased while follicle-stimulating hormone (FSH) decreased; 
      ovarian structure improved and follicle number increased; ovarian expression of 
      HGF, VEGF, and IGF-1 protein elevated significantly. Meanwhile, PCR and western 
      blot analysis indicated hUCMSCs have the capacity of secreting HGF, VEGF, and 
      IGF-1 cytokines. CONCLUSIONS: Our results suggest that hUCMSCs can promote 
      ovarian expression of HGF, VEGF, and IGF-1 through secreting those cytokines, 
      resulting in improving ovarian reserve function and withstanding ovarian 
      senescence.
FAU - Li, Jia
AU  - Li J
AD  - Department of Obstetrics and Gynecology, Graduate College, Guangdong Medical 
      University, Zhanjiang, Guangdong, 524023, China.
AD  - Department of Obstetrics and Gynecology, Guangdong No.2 Provincial People's 
      Hospital, NO.466 Xingangdong Road, Guangzhou, 510317, China.
FAU - Mao, QiuXian
AU  - Mao Q
AD  - Department of Obstetrics and Gynecology, Guangdong No.2 Provincial People's 
      Hospital, NO.466 Xingangdong Road, Guangzhou, 510317, China.
FAU - He, JingJun
AU  - He J
AD  - Department of Physical Examination, Guangdong No.2 Provincial People's Hospital, 
      NO.466 Xingangdong Road, Guangzhou, 510317, China.
FAU - She, HaoQing
AU  - She H
AD  - Department of Obstetrics and Gynecology, Medical College, NanHua University, 
      Hengyang, Hunan, 421001, China.
FAU - Zhang, Zhi
AU  - Zhang Z
AD  - Department of Laboratory Medicine, Guangdong No.2 Provincial People's Hospital, 
      NO.466 Xingangdong Road, Guangzhou, 510317, China. yalw135@126.com.
FAU - Yin, ChunYan
AU  - Yin C
AD  - Department of Obstetrics and Gynecology, Guangdong No.2 Provincial People's 
      Hospital, NO.466 Xingangdong Road, Guangzhou, 510317, China. cy_yin@vip.163.com.
LA  - eng
PT  - Journal Article
DEP - 20170309
PL  - England
TA  - Stem Cell Res Ther
JT  - Stem cell research & therapy
JID - 101527581
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (vascular endothelial growth factor A, rat)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
RN  - 9002-68-0 (Follicle Stimulating Hormone)
SB  - IM
MH  - Animals
MH  - Apoptosis/genetics
MH  - Cell Proliferation/genetics
MH  - Cellular Senescence/genetics
MH  - Coculture Techniques
MH  - Female
MH  - Follicle Stimulating Hormone/*genetics
MH  - Gene Expression Regulation, Developmental
MH  - Hepatocyte Growth Factor/*genetics
MH  - Humans
MH  - Insulin-Like Growth Factor I/*genetics
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/cytology
MH  - Ovarian Follicle/*growth & development/metabolism
MH  - Ovarian Reserve/genetics
MH  - Paracrine Communication/genetics
MH  - Perimenopause
MH  - Rats
MH  - Umbilical Cord/cytology
MH  - Vascular Endothelial Growth Factor A/*genetics
PMC - PMC5345137
OTO - NOTNLM
OT  - HGF
OT  - IGF-1
OT  - Ovarian reserve function
OT  - Paracrine
OT  - Perimenopausal
OT  - VEGF
OT  - hUCMSCs
EDAT- 2017/03/11 06:00
MHDA- 2017/11/29 06:00
PMCR- 2017/03/09
CRDT- 2017/03/11 06:00
PHST- 2016/10/14 00:00 [received]
PHST- 2017/02/18 00:00 [accepted]
PHST- 2017/01/05 00:00 [revised]
PHST- 2017/03/11 06:00 [entrez]
PHST- 2017/03/11 06:00 [pubmed]
PHST- 2017/11/29 06:00 [medline]
PHST- 2017/03/09 00:00 [pmc-release]
AID - 10.1186/s13287-017-0514-5 [pii]
AID - 514 [pii]
AID - 10.1186/s13287-017-0514-5 [doi]
PST - epublish
SO  - Stem Cell Res Ther. 2017 Mar 9;8(1):55. doi: 10.1186/s13287-017-0514-5.