PMID- 28280257
OWN - NLM
STAT- MEDLINE
DCOM- 20170601
LR  - 20231112
IS  - 2325-4416 (Electronic)
IS  - 2325-4394 (Print)
IS  - 2325-4394 (Linking)
VI  - 23
DP  - 2017 Mar 10
TI  - Intermediate-Conductance-Ca2-Activated K Channel IKCa1 Is Upregulated and 
      Promotes Cell Proliferation in Cervical Cancer.
PG  - 45-57
AB  - BACKGROUND Accumulating data point to intermediate-conductance calcium-activated 
      potassium channel (IKCa1) as a key player in controlling cell cycle progression 
      and proliferation of human cancer cells. However, the role that IKCa1 plays in 
      the growth of human cervical cancer cells is largely unexplored. MATERIAL AND 
      METHODS In this study, Western blot analysis, immunohistochemical staining, and 
      RT-PCR were first used for IKCa1protein and gene expression assays in cervical 
      cancer tissues and HeLa cells. Then, IKCa1 channel blocker and siRNA were 
      employed to inhibit the functionality of IKCa1 and downregulate gene expression 
      in HeLa cells, respectively. After these treatments, we examined the level of 
      cell proliferation by MTT method and measured IKCa1 currents by conventional 
      whole-cell patch clamp technique. Cell apoptosis was assessed using the Annexin 
      V-FITC/Propidium Iodide (PI) double-staining apoptosis detection kit. RESULTS We 
      demonstrated that IKCa1 mRNA and protein are preferentially expressed in cervical 
      cancer tissues and HeLa cells. We also showed that the IKCa1 channel blocker, 
      clotrimazole, and IKCa1 channel siRNA can be used to suppress cervical cancer 
      cell proliferation and decrease IKCa1 channel current. IKCa1 downregulation by 
      specific siRNAs induced a significant increase in the proportion of apoptotic 
      cells in HeLa cells. CONCLUSIONS IKCa1 is overexpressed in cervical cancer 
      tissues, and IKCa1 upregulation in cervical cancer cell linea enhances cell 
      proliferation, partly by reducing the proportion of apoptotic cells.
FAU - Liu, Ling
AU  - Liu L
AD  - Department of Gynaecology and Obstetrics, The Affiliated Hospital of Southwest 
      Medical University, Luzhou, Sichuan, China (mainland).
FAU - Zhan, Ping
AU  - Zhan P
AD  - Department of Gynaecology and Obstetrics, The Affiliated Hospital of Southwest 
      Medical University, Luzhou, Sichuan, China (mainland).
FAU - Nie, Dan
AU  - Nie D
AD  - Department of Gynaecology and Obstetrics, The Affiliated Hospital of Southwest 
      Medical University, Luzhou, Sichuan, China (mainland).
FAU - Fan, Lingye
AU  - Fan L
AD  - Department of Gynaecology and Obstetrics, The Affiliated Hospital of Southwest 
      Medical University, Luzhou, Sichuan, China (mainland).
FAU - Lin, Hairui
AU  - Lin H
AD  - Department of Gynaecology and Obstetrics, The Affiliated Hospital of Southwest 
      Medical University, Luzhou, Sichuan, China (mainland).
FAU - Gao, Lanyang
AU  - Gao L
AD  - Department of Gynaecology and Obstetrics, The Affiliated Hospital of Southwest 
      Medical University, Luzhou, Sichuan, China (mainland).
FAU - Mao, Xiguang
AU  - Mao X
AD  - Department of Gynaecology and Obstetrics, The Affiliated Hospital of Southwest 
      Medical University, Luzhou, Sichuan, China (mainland).
LA  - eng
PT  - Journal Article
DEP - 20170310
PL  - United States
TA  - Med Sci Monit Basic Res
JT  - Medical science monitor basic research
JID - 101597444
RN  - 0 (Calcium Channel Blockers)
RN  - 0 (Intermediate-Conductance Calcium-Activated Potassium Channels)
RN  - 0 (KCNN4 protein, human)
RN  - 0 (RNA, Messenger)
RN  - G07GZ97H65 (Clotrimazole)
SB  - IM
MH  - Adult
MH  - Calcium Channel Blockers/pharmacology
MH  - Cell Proliferation/drug effects/physiology
MH  - Clotrimazole/pharmacology
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - HeLa Cells
MH  - Humans
MH  - Intermediate-Conductance Calcium-Activated Potassium 
      Channels/biosynthesis/genetics/*metabolism
MH  - Middle Aged
MH  - Patch-Clamp Techniques
MH  - RNA, Messenger/genetics/metabolism
MH  - Uterine Cervical Neoplasms/genetics/*metabolism/*pathology
PMC - PMC5358865
COIS- Conflicts of interest No potential conflicts of interest were disclosed.
EDAT- 2017/03/11 06:00
MHDA- 2017/06/02 06:00
PMCR- 2017/03/10
CRDT- 2017/03/11 06:00
PHST- 2017/03/11 06:00 [entrez]
PHST- 2017/03/11 06:00 [pubmed]
PHST- 2017/06/02 06:00 [medline]
PHST- 2017/03/10 00:00 [pmc-release]
AID - 901462 [pii]
AID - 10.12659/msmbr.901462 [doi]
PST - epublish
SO  - Med Sci Monit Basic Res. 2017 Mar 10;23:45-57. doi: 10.12659/msmbr.901462.