PMID- 28280413
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200929
IS  - 1226-4512 (Print)
IS  - 2093-3827 (Electronic)
IS  - 1226-4512 (Linking)
VI  - 21
IP  - 2
DP  - 2017 Mar
TI  - Effect of oleanolic acid on the activity, secretion and gene expression of matrix 
      metalloproteinase-3 in articular chondrocytes in vitro and the production of 
      matrix metalloproteinase-3 in vivo.
PG  - 197-204
LID - 10.4196/kjpp.2017.21.2.197 [doi]
AB  - In the present study, we tried to examine whether oleanolic acid regulates the 
      activity, secretion and gene expression of matrix metalloproteinase-3 (MMP-3) in 
      primary cultured rabbit articular chondrocytes, as well as the production of 
      MMP-3 in the knee joint of rat to evaluate the potential chondroprotective effect 
      of oleanolic acid. Rabbit articular chondrocytes were cultured in a monolayer, 
      and reverse transcription-polymerase chain reaction (RT-PCR) was used to measure 
      interleukin-1beta (IL-1beta)-induced gene expression of MMP-3, MMP-1, MMP-13, a 
      disintegrin and metalloproteinase with thrombospondin motifs-4 (ADAMTS-4), 
      ADAMTS-5 and type II collagen. In rabbit articular chondrocytes, the effects of 
      oleanolic acid on IL-1beta-induced secretion and proteolytic activity of MMP-3 were 
      investigated using western blot analysis and casein zymography, respectively. The 
      effect of oleanolic acid on in vivo MMP-3 protein production was also examined, 
      after intra-articular injection to the knee joint of rat. The results were as 
      follows: (1) oleanolic acid inhibited the gene expression of MMP-3, MMP-1, 
      MMP-13, ADAMTS-4, and ADAMTS-5, but increased the gene expression of type II 
      collagen; (2) oleanolic acid reduced the secretion and proteolytic activity of 
      MMP-3; (3) oleanolic acid suppressed the production of MMP-3 protein in vivo. 
      These results suggest that oleanolic acid can regulate the activity, secretion 
      and gene expression of MMP-3, by directly acting on articular chondrocytes.
FAU - Kang, Dong-Geun
AU  - Kang DG
AD  - Department of Orthopedic Surgery and Institute of Health Sciences, School of 
      Medicine and Hospital, Gyeongsang National University, Changwon 51472, Korea.
FAU - Lee, Hyun Jae
AU  - Lee HJ
AD  - Department of Health Management, Sahmyook University, Seoul 01795, Korea.
FAU - Kim, Kun Tae
AU  - Kim KT
AD  - Department of Orthopedic Surgery and Institute of Health Sciences, School of 
      Medicine and Hospital, Gyeongsang National University, Jinju 52727, Korea.
FAU - Hwang, Sun-Chul
AU  - Hwang SC
AD  - Department of Orthopedic Surgery and Institute of Health Sciences, School of 
      Medicine and Hospital, Gyeongsang National University, Jinju 52727, Korea.
FAU - Lee, Choong Jae
AU  - Lee CJ
AD  - Department of Pharmacology, School of Medicine, Chungnam National University, 
      Daejeon 35015, Korea.
FAU - Park, Jin Sung
AU  - Park JS
AD  - Department of Orthopedic Surgery and Institute of Health Sciences, School of 
      Medicine and Hospital, Gyeongsang National University, Jinju 52727, Korea.
LA  - eng
PT  - Journal Article
DEP - 20170221
PL  - Korea (South)
TA  - Korean J Physiol Pharmacol
JT  - The Korean journal of physiology & pharmacology : official journal of the Korean 
      Physiological Society and the Korean Society of Pharmacology
JID - 9709505
PMC - PMC5343053
OTO - NOTNLM
OT  - Chondrocytes
OT  - MMPs
OT  - Oleanolic acid
OT  - Osteoarthritis
COIS- CONFLICTS OF INTEREST: The authors declare no conflicts of interest.
EDAT- 2017/03/11 06:00
MHDA- 2017/03/11 06:01
PMCR- 2017/03/01
CRDT- 2017/03/11 06:00
PHST- 2016/11/03 00:00 [received]
PHST- 2016/11/24 00:00 [revised]
PHST- 2016/12/01 00:00 [accepted]
PHST- 2017/03/11 06:00 [entrez]
PHST- 2017/03/11 06:00 [pubmed]
PHST- 2017/03/11 06:01 [medline]
PHST- 2017/03/01 00:00 [pmc-release]
AID - 10.4196/kjpp.2017.21.2.197 [doi]
PST - ppublish
SO  - Korean J Physiol Pharmacol. 2017 Mar;21(2):197-204. doi: 
      10.4196/kjpp.2017.21.2.197. Epub 2017 Feb 21.