PMID- 2828078
OWN - NLM
STAT- MEDLINE
DCOM- 19880307
LR  - 20190624
IS  - 0014-2999 (Print)
IS  - 0014-2999 (Linking)
VI  - 142
IP  - 3
DP  - 1987 Oct 27
TI  - Pregnenolone and its sulphate modulate GABAA-receptor-mediated contractile 
      responses in the guinea-pig isolated ileum.
PG  - 461-4
AB  - Pregnenolone and its sulphate had concentration-dependent dual actions on 
      gamma-aminobutyric acid (GABAA)-receptor-mediated ileal contractile responses, 
      enhancing at 0.001-1 microM but inhibiting at 10 microM. There was a sinistral 
      shift of the GABA dose-response curve in the presence of 0.01 microM 
      pregnenolone, with a significant potentiation over the lower concentration range 
      of GABA (3-30 microM), whilst 10 microM pregnenolone depressed the curve by 50% 
      without a significant change in the EC50, in a manner resembling non-competitive 
      inhibition with picrotoxin. Pregnenolone and its sulphate evidently have 
      modulatory actions at ileal GABAA-receptor complexes in keeping with those seen 
      using neurochemical studies in the central nervous system.
FAU - Ong, J
AU  - Ong J
AD  - Department of Pharmacology, University of Sydney, New South Wales, Australia.
FAU - Kerr, D I
AU  - Kerr DI
FAU - Johnston, G A
AU  - Johnston GA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Eur J Pharmacol
JT  - European journal of pharmacology
JID - 1254354
RN  - 0 (Receptors, GABA-A)
RN  - 04Y4D91RG0 (pregnenolone sulfate)
RN  - 73R90F7MQ8 (Pregnenolone)
SB  - IM
MH  - Animals
MH  - Female
MH  - Guinea Pigs
MH  - Ileum/physiology
MH  - In Vitro Techniques
MH  - Male
MH  - Muscle Contraction/drug effects
MH  - Muscle, Smooth/*physiology
MH  - Pregnenolone/*pharmacology
MH  - Receptors, GABA-A/*physiology
EDAT- 1987/10/27 00:00
MHDA- 1987/10/27 00:01
CRDT- 1987/10/27 00:00
PHST- 1987/10/27 00:00 [pubmed]
PHST- 1987/10/27 00:01 [medline]
PHST- 1987/10/27 00:00 [entrez]
AID - 0014-2999(87)90089-6 [pii]
AID - 10.1016/0014-2999(87)90089-6 [doi]
PST - ppublish
SO  - Eur J Pharmacol. 1987 Oct 27;142(3):461-4. doi: 10.1016/0014-2999(87)90089-6.