PMID- 28281023
OWN - NLM
STAT- MEDLINE
DCOM- 20171013
LR  - 20181113
IS  - 1573-6776 (Electronic)
IS  - 0141-5492 (Print)
IS  - 0141-5492 (Linking)
VI  - 39
IP  - 5
DP  - 2017 May
TI  - Modification of fatty acid selectivity of Candida antarctica lipase A by 
      error-prone PCR.
PG  - 767-773
LID - 10.1007/s10529-017-2299-0 [doi]
AB  - OBJECTIVE: To generate Candida antarctica lipase A (CAL-A) mutants with modified 
      fatty acid selectivities and improved lipolytic activities using error-prone PCR 
      (epPCR). RESULTS: A Candida antarctica lipase A mutant was obtained in three 
      rounds of epPCR. This mutant showed a 14 times higher ability to hydrolyze 
      triacylglycerols containing conjugated linoleic acids, and was 12 and 14 times 
      more selective towards cis-9, trans-11 and trans-10, cis-12 isomers respectively, 
      compared to native lipase. Lipolytic activities towards fatty acid esters were 
      markedly improved, in particular towards butyric, lauric, stearic and palmitic 
      esters. CONCLUSION: Directed molecular evolution is an efficient method to 
      generate lipases with desirable selectivity towards CLA isomers and improved 
      lipolytic activities towards esters of fatty acids.
FAU - Glod, Dagmara
AU  - Glod D
AD  - Department of Food Biotechnology, University of Warmia and Mazury in Olsztyn, 
      Heweliusz 1 Str., 10-718, Olsztyn, Poland. dagmara.glod@uwm.edu.pl.
AD  - Department of Pathophysiology, University of Warmia and Mazury in Olsztyn, al. 
      Warszawska 30, 10-082, Olsztyn, Poland. dagmara.glod@uwm.edu.pl.
LA  - eng
PT  - Journal Article
DEP - 20170309
PL  - Netherlands
TA  - Biotechnol Lett
JT  - Biotechnology letters
JID - 8008051
RN  - 0 (Fungal Proteins)
RN  - 0 (Linoleic Acids, Conjugated)
RN  - 0 (Triglycerides)
RN  - EC 3.1.1.3 (Lipase)
SB  - IM
MH  - *Candida/enzymology/genetics/metabolism
MH  - Directed Molecular Evolution/*methods
MH  - Fungal Proteins/genetics/*metabolism
MH  - Linoleic Acids, Conjugated/analysis/chemistry/*metabolism
MH  - Lipase/genetics/*metabolism
MH  - Polymerase Chain Reaction/methods
MH  - Protein Engineering/methods
MH  - Triglycerides/analysis/chemistry/metabolism
PMC - PMC5409807
OTO - NOTNLM
OT  - Conjugated linoleic acid
OT  - Directed molecular evolution
OT  - Error-prone PCR
OT  - Lipase
OT  - Protein engineering
OT  - Selectivity
COIS- The author declares no financial or commercial conflict of interest.
EDAT- 2017/03/11 06:00
MHDA- 2017/10/14 06:00
PMCR- 2017/03/09
CRDT- 2017/03/11 06:00
PHST- 2016/10/07 00:00 [received]
PHST- 2017/02/02 00:00 [accepted]
PHST- 2017/03/11 06:00 [pubmed]
PHST- 2017/10/14 06:00 [medline]
PHST- 2017/03/11 06:00 [entrez]
PHST- 2017/03/09 00:00 [pmc-release]
AID - 10.1007/s10529-017-2299-0 [pii]
AID - 2299 [pii]
AID - 10.1007/s10529-017-2299-0 [doi]
PST - ppublish
SO  - Biotechnol Lett. 2017 May;39(5):767-773. doi: 10.1007/s10529-017-2299-0. Epub 
      2017 Mar 9.