PMID- 28281257
OWN - NLM
STAT- MEDLINE
DCOM- 20180226
LR  - 20180607
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1571
DP  - 2017
TI  - DNA-Directed Antibody Immobilization for Robust Protein Microarrays: Application 
      to Single Particle Detection 'DNA-Directed Antibody Immobilization.
PG  - 187-206
LID - 10.1007/978-1-4939-6848-0_12 [doi]
AB  - Protein microarrays are emerging tools which have become very powerful in 
      multiplexed detection technologies. A variety of proteins can be immobilized on a 
      sensor chip allowing for multiplexed diagnostics. Therefore, various types of 
      analyte in a small volume of sample can be detected simultaneously. Protein 
      immobilization is a crucial step for creating a robust and sensitive protein 
      microarray-based detection system. In order to achieve a successful protein 
      immobilization and preserve the activity of the proteins after immobilization, 
      DNA-directed immobilization is a promising technique. Here, we present the design 
      and the use of DNA-directed immobilized (DDI) antibodies in fabrication of robust 
      protein microarrays. We focus on application of protein microarrays for capturing 
      and detecting nanoparticles such as intact viruses. Experimental results on 
      Single-particle interferometric reflectance imaging sensor (SP-IRIS) are used to 
      validate the advantages of the DDI method.
FAU - Unlu, Nese Lortlar
AU  - Unlu NL
AD  - Biomedical Engineering Department, Boston University, Boston, MA, 02215, USA.
AD  - Faculty of Medicine, Bahcesehir University, Istanbul, 34353, Turkey.
FAU - Kanik, Fulya Ekiz
AU  - Kanik FE
AD  - Electrical and Computer Engineering Department, Boston University, Boston, MA, 
      02215, USA.
FAU - Seymour, Elif
AU  - Seymour E
AD  - Biotechnology Research Program Department, ASELSAN Research Center, Ankara, 
      06370, Turkey.
FAU - Connor, John H
AU  - Connor JH
AD  - Microbiology Department, Boston University School of Medicine, 8 St Mary's St, 
      Boston, MA, 02215, USA.
FAU - Unlu, M Selim
AU  - Unlu MS
AD  - Biomedical Engineering Department, Boston University, Boston, MA, 02215, USA. 
      selim@bu.edu.
AD  - Electrical and Computer Engineering Department, Boston University, Boston, MA, 
      02215, USA. selim@bu.edu.
AD  - Microbiology Department, Boston University School of Medicine, 8 St Mary's St, 
      Boston, MA, 02215, USA. selim@bu.edu.
LA  - eng
GR  - R01 AI096159/AI/NIAID NIH HHS/United States
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Antibodies, Immobilized)
RN  - 0 (DNA Probes)
RN  - 0 (Immunoconjugates)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - *Antibodies, Immobilized
MH  - Biosensing Techniques/instrumentation/*methods
MH  - *DNA
MH  - DNA Probes
MH  - Image Processing, Computer-Assisted/methods
MH  - Immunoconjugates
MH  - Microfluidics/instrumentation
MH  - Microscopy/methods
MH  - Molecular Imaging/instrumentation/*methods
MH  - Protein Array Analysis/instrumentation/*methods
MH  - Statistics as Topic/methods
MH  - Viruses/immunology
OTO - NOTNLM
OT  - DNA-directed antibody immobilization
OT  - Label free detection
OT  - Protein microarrays
OT  - SP-IRIS
OT  - Single particle detection
EDAT- 2017/03/11 06:00
MHDA- 2018/02/27 06:00
CRDT- 2017/03/11 06:00
PHST- 2017/03/11 06:00 [entrez]
PHST- 2017/03/11 06:00 [pubmed]
PHST- 2018/02/27 06:00 [medline]
AID - 10.1007/978-1-4939-6848-0_12 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2017;1571:187-206. doi: 10.1007/978-1-4939-6848-0_12.