PMID- 28281841
OWN - NLM
STAT- MEDLINE
DCOM- 20180501
LR  - 20180501
IS  - 1532-4206 (Electronic)
IS  - 0743-5800 (Linking)
VI  - 42
IP  - 3
DP  - 2017 Aug
TI  - LncRNA NONRATT021972 siRNA normalized the dysfunction of hepatic glucokinase 
      through AKT signaling in T2DM rats.
PG  - 180-190
LID - 10.1080/07435800.2017.1292522 [doi]
AB  - Hepatic glucokinase (GK) expression and activity are decreased in type 2 diabetes 
      mellitus (T2DM), and glycogen synthase kinase-3 (GSK-3) inhibits the synthesis of 
      GK. In hepatocytes, the activation of the protein kinase B (PKB/AKT) signaling 
      pathway enhances GK expression and inhibits the phosphorylation of GSK-3beta. The 
      dysfunction of certain long noncoding RNAs (lncRNAs) has been associated with a 
      variety of diseases. AIMS: This study explored the effects of the lncRNA 
      NONRATT021972 small interfering RNA (siRNA) on the dysfunction of hepatic GK 
      through AKT signaling in T2DM rats. METHODS: Livers from type 2 diabetic rats and 
      hepatocytes cultured in high glucose and high fatty acid media were studied. The 
      changes in expression of AKT, GK and GSK 3beta were detected by western blotting or 
      RT-PCR. The application of bioinformatics technology (CatRAPID) was used to 
      identify the targets of NONRATT021972 RNA. RESULTS: We found that lncRNA 
      NONRATT021972 levels in the liver were increased in type 2 diabetic rats, and the 
      increase was associated with an increase in the blood glucose levels. The 
      NONRATT021972 siRNA enhanced phospho-AKT (p-AKT) levels, GK expression and 
      hepatic glycogen synthesis. This siRNA also reduced phospho-glycogen synthase 
      kinase-3beta (p-GSK-3beta) levels and hyperglycemia in T2DM rats, as well as in 
      hepatocytes cultured in high glucose media with fatty acids. CatRAPID predicted 
      that there was the interaction between NONRATT021972 and p-AKT. CONCLUSIONS: 
      LncRNA NONRATT021972 siRNA may have beneficial effects on T2DM.
FAU - Song, Miaomiao
AU  - Song M
AD  - a Department of Physiology , Basic Medical College of Nanchang University , 
      Nanchang , Jiangxi , People's Republic of China.
FAU - Zou, Lifang
AU  - Zou L
AD  - a Department of Physiology , Basic Medical College of Nanchang University , 
      Nanchang , Jiangxi , People's Republic of China.
FAU - Peng, Lichao
AU  - Peng L
AD  - a Department of Physiology , Basic Medical College of Nanchang University , 
      Nanchang , Jiangxi , People's Republic of China.
FAU - Liu, Shuangmei
AU  - Liu S
AD  - a Department of Physiology , Basic Medical College of Nanchang University , 
      Nanchang , Jiangxi , People's Republic of China.
FAU - Wu, Bing
AU  - Wu B
AD  - a Department of Physiology , Basic Medical College of Nanchang University , 
      Nanchang , Jiangxi , People's Republic of China.
FAU - Yi, Zhihua
AU  - Yi Z
AD  - a Department of Physiology , Basic Medical College of Nanchang University , 
      Nanchang , Jiangxi , People's Republic of China.
FAU - Gao, Yun
AU  - Gao Y
AD  - a Department of Physiology , Basic Medical College of Nanchang University , 
      Nanchang , Jiangxi , People's Republic of China.
FAU - Zhang, Chunping
AU  - Zhang C
AD  - b Department of Cell Biology , Basic Medical College of Nanchang University , 
      Nanchang , Jiangxi , People's Republic of China.
FAU - Xu, Hong
AU  - Xu H
AD  - a Department of Physiology , Basic Medical College of Nanchang University , 
      Nanchang , Jiangxi , People's Republic of China.
FAU - Xu, Yurong
AU  - Xu Y
AD  - a Department of Physiology , Basic Medical College of Nanchang University , 
      Nanchang , Jiangxi , People's Republic of China.
FAU - Tang, Mengxia
AU  - Tang M
AD  - a Department of Physiology , Basic Medical College of Nanchang University , 
      Nanchang , Jiangxi , People's Republic of China.
FAU - Wang, Shouyu
AU  - Wang S
AD  - a Department of Physiology , Basic Medical College of Nanchang University , 
      Nanchang , Jiangxi , People's Republic of China.
FAU - Xue, Yun
AU  - Xue Y
AD  - a Department of Physiology , Basic Medical College of Nanchang University , 
      Nanchang , Jiangxi , People's Republic of China.
FAU - Jia, Tianyu
AU  - Jia T
AD  - a Department of Physiology , Basic Medical College of Nanchang University , 
      Nanchang , Jiangxi , People's Republic of China.
FAU - Zhao, Shanhong
AU  - Zhao S
AD  - a Department of Physiology , Basic Medical College of Nanchang University , 
      Nanchang , Jiangxi , People's Republic of China.
FAU - Liang, Shangdong
AU  - Liang S
AD  - a Department of Physiology , Basic Medical College of Nanchang University , 
      Nanchang , Jiangxi , People's Republic of China.
FAU - Li, Guilin
AU  - Li G
AD  - a Department of Physiology , Basic Medical College of Nanchang University , 
      Nanchang , Jiangxi , People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20170310
PL  - England
TA  - Endocr Res
JT  - Endocrine research
JID - 8408548
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (long non-coding RNA NONRATT021972, rat)
RN  - EC 2.7.1.2 (Glucokinase)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
SB  - IM
MH  - Animals
MH  - Diabetes Mellitus, Experimental/*metabolism
MH  - Diabetes Mellitus, Type 2/*metabolism
MH  - Glucokinase/*metabolism
MH  - Male
MH  - Proto-Oncogene Proteins c-akt/*metabolism
MH  - RNA, Long Noncoding/*metabolism
MH  - RNA, Small Interfering/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Signal Transduction/*physiology
OTO - NOTNLM
OT  - AKT
OT  - Glucokinase
OT  - Type 2 diabetes
OT  - long noncoding RNA
EDAT- 2017/03/11 06:00
MHDA- 2018/05/02 06:00
CRDT- 2017/03/11 06:00
PHST- 2017/03/11 06:00 [pubmed]
PHST- 2018/05/02 06:00 [medline]
PHST- 2017/03/11 06:00 [entrez]
AID - 10.1080/07435800.2017.1292522 [doi]
PST - ppublish
SO  - Endocr Res. 2017 Aug;42(3):180-190. doi: 10.1080/07435800.2017.1292522. Epub 2017 
      Mar 10.