PMID- 2828365
OWN - NLM
STAT- MEDLINE
DCOM- 19880323
LR  - 20210210
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 263
IP  - 5
DP  - 1988 Feb 15
TI  - A 55,000-60,000 Mr receptor protein for urokinase-type plasminogen activator. 
      Identification in human tumor cell lines and partial purification.
PG  - 2358-63
AB  - The iodinated Mr approximately equal to 15,000 amino-terminal fragment (ATF) of 
      the urokinase-type plasminogen activator (u-PA) molecule bound specifically to 
      the cell surface of all of seven cultured human tumor cell lines studied. 
      Cross-linking of iodinated ATF to the cell surface using a bifunctional 
      amino-reactive reagent followed by sodium dodecyl sulfate-polyacrylamide gel 
      electrophoresis and autoradiography revealed with the four cell lines studied the 
      occurrence of a single band migrating with an Mr of 70,000-75,000, indicating 
      complex formation with an Mr of 55,000-60,000 u-PA receptor protein (u-PA-R). In 
      the human monocyte cell line U937 cultivated in the presence of phorbol ester, 
      the amount of complex was strongly increased, and a fraction of the complex had a 
      slower electrophoretic mobility. Comparison between autoradiograms of reduced and 
      unreduced samples suggests that u-PA-R consists of one polypeptide chain. Two 
      forms of u-PA-R, which differed with respect to affinity to concanavalin A, were 
      identified. u-PA-R retained its ability to bind to ATF after cell lysis, and it 
      was purified approximately 2,200-fold from biosynthetically labeled U937 cells by 
      affinity chromatography with proenzyme u-PA coupled to Sepharose. The purified Mr 
      55,000-60,000 protein was specifically bound and cross-linked to u-PA, proenzyme 
      u-PA, and ATF, but not to tissue-type plasminogen activator or other unrelated 
      proteins.
FAU - Nielsen, L S
AU  - Nielsen LS
AD  - Finsen Laboratory, Rigshospitalet, Copenhagen, Denmark.
FAU - Kellerman, G M
AU  - Kellerman GM
FAU - Behrendt, N
AU  - Behrendt N
FAU - Picone, R
AU  - Picone R
FAU - Dano, K
AU  - Dano K
FAU - Blasi, F
AU  - Blasi F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (PLAUR protein, human)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Urokinase Plasminogen Activator)
RN  - EC 3.4.21.- (Plasminogen Activators)
RN  - EC 3.4.21.73 (Urokinase-Type Plasminogen Activator)
SB  - IM
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Humans
MH  - Kinetics
MH  - Molecular Weight
MH  - Plasminogen Activators/*metabolism
MH  - Receptors, Cell Surface/isolation & purification/*metabolism
MH  - Receptors, Urokinase Plasminogen Activator
MH  - Tumor Cells, Cultured/*metabolism
MH  - Urokinase-Type Plasminogen Activator/*metabolism
EDAT- 1988/02/15 00:00
MHDA- 1988/02/15 00:01
CRDT- 1988/02/15 00:00
PHST- 1988/02/15 00:00 [pubmed]
PHST- 1988/02/15 00:01 [medline]
PHST- 1988/02/15 00:00 [entrez]
AID - S0021-9258(18)69214-2 [pii]
PST - ppublish
SO  - J Biol Chem. 1988 Feb 15;263(5):2358-63.