PMID- 28284075
OWN - NLM
STAT- MEDLINE
DCOM- 20171129
LR  - 20171129
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 94
DP  - 2017 Aug 15
TI  - A fluorogenic probe for imaging protein S-nitrosylation in live cells.
PG  - 162-168
LID - S0956-5663(17)30148-3 [pii]
LID - 10.1016/j.bios.2017.02.050 [doi]
AB  - S-nitrosylation is a posttranslational modification of protein cysteine residues 
      leading to the formation of S-nitrosothiols and its detection is crucial to 
      understanding of redox regulation and NO-based signaling. Prototypical detection 
      methods for S-nitrosylation are always carried out ex situ. However, the 
      reversible nature and the tendency of transnitrosylation highlight the necessity 
      of its probing in intact live biological contexts. Herein we provide a 
      fluorogenic chemical probe for the detection of S-nitrosylation in live 
      endothelial cells. The probe is weakly emissive alone and becomes highly 
      fluorescent only after undergoing a reaction with S-nitrosothiols in live 
      cellular environments. This probe features high degrees of specificity and 
      desirable sensitivity. Furthermore, it has been successfully applied to image the 
      dynamic change of protein S-nitrosylation in live endothelial cells. The 
      applicability of the probe in complex biological systems has been additionally 
      verified by imaging a known target of S-nitrosylation, glyceraldehyde-3-phosphate 
      dehydrogenase (GAPDH), in live cells. Due to the versatility exemplified, this 
      probe holds great promise for exploring the role of protein S-nitrosylation in 
      the pathophysiological process of a variety of vascular diseases.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Shao, Shiyi
AU  - Shao S
AD  - College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
FAU - Chen, Bo
AU  - Chen B
AD  - Department of Chemistry, Zhejiang Sci-Tech University, Hangzhou 310018, China.
FAU - Cheng, Juan
AU  - Cheng J
AD  - College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
FAU - Wang, Chengkun
AU  - Wang C
AD  - College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
FAU - Zhang, Yanli
AU  - Zhang Y
AD  - College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
FAU - Shao, Lingxiao
AU  - Shao L
AD  - College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
FAU - Hu, Yongzhou
AU  - Hu Y
AD  - College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
FAU - Han, Yifeng
AU  - Han Y
AD  - Department of Chemistry, Zhejiang Sci-Tech University, Hangzhou 310018, China.
FAU - Han, Feng
AU  - Han F
AD  - College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China. 
      Electronic address: changhuahan@zju.edu.cn.
FAU - Li, Xin
AU  - Li X
AD  - College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China. 
      Electronic address: lixin81@zju.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20170301
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (Protein S)
RN  - 0 (S-Nitrosothiols)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
RN  - K848JZ4886 (Cysteine)
SB  - IM
MH  - Biosensing Techniques/*methods
MH  - Cysteine/chemistry
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/*chemistry
MH  - Humans
MH  - Nitric Oxide/*metabolism
MH  - Oxidation-Reduction
MH  - Protein Processing, Post-Translational/genetics
MH  - Protein S/chemistry/*isolation & purification
MH  - S-Nitrosothiols/chemistry
MH  - Signal Transduction/genetics
OTO - NOTNLM
OT  - Endothelial cell
OT  - Fluorescent probe
OT  - Imaging
OT  - Posttranslational modification
OT  - S-nitrosylation
EDAT- 2017/03/12 06:00
MHDA- 2017/12/01 06:00
CRDT- 2017/03/12 06:00
PHST- 2016/12/23 00:00 [received]
PHST- 2017/02/22 00:00 [revised]
PHST- 2017/02/28 00:00 [accepted]
PHST- 2017/03/12 06:00 [pubmed]
PHST- 2017/12/01 06:00 [medline]
PHST- 2017/03/12 06:00 [entrez]
AID - S0956-5663(17)30148-3 [pii]
AID - 10.1016/j.bios.2017.02.050 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2017 Aug 15;94:162-168. doi: 10.1016/j.bios.2017.02.050. 
      Epub 2017 Mar 1.