PMID- 28284415
OWN - NLM
STAT- MEDLINE
DCOM- 20180403
LR  - 20220408
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 209
DP  - 2017 Sep
TI  - Sampling guidelines for oral fluid-based surveys of group-housed animals.
PG  - 20-29
LID - S0378-1135(16)30527-2 [pii]
LID - 10.1016/j.vetmic.2017.02.004 [doi]
AB  - Formulas and software for calculating sample size for surveys based on individual 
      animal samples are readily available. However, sample size formulas are not 
      available for oral fluids and other aggregate samples that are increasingly used 
      in production settings. Therefore, the objective of this study was to develop 
      sampling guidelines for oral fluid-based porcine reproductive and respiratory 
      syndrome virus (PRRSV) surveys in commercial swine farms. Oral fluid samples were 
      collected in 9 weekly samplings from all pens in 3 barns on one production site 
      beginning shortly after placement of weaned pigs. Samples (n=972) were tested by 
      real-time reverse-transcription PCR (RT-rtPCR) and the binary results analyzed 
      using a piecewise exponential survival model for interval-censored, time-to-event 
      data with misclassification. Thereafter, simulation studies were used to study 
      the barn-level probability of PRRSV detection as a function of sample size, 
      sample allocation (simple random sampling vs fixed spatial sampling), assay 
      diagnostic sensitivity and specificity, and pen-level prevalence. These studies 
      provided estimates of the probability of detection by sample size and within-barn 
      prevalence. Detection using fixed spatial sampling was as good as, or better 
      than, simple random sampling. Sampling multiple barns on a site increased the 
      probability of detection with the number of barns sampled. These results are 
      relevant to PRRSV control or elimination projects at the herd, regional, or 
      national levels, but the results are also broadly applicable to contagious 
      pathogens of swine for which oral fluid tests of equivalent performance are 
      available.
CI  - Copyright (c) 2017 The Authors. Published by Elsevier B.V. All rights reserved.
FAU - Rotolo, Marisa L
AU  - Rotolo ML
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA, USA. Electronic address: 
      mrotolo@iastate.edu.
FAU - Sun, Yaxuan
AU  - Sun Y
AD  - Department of Statistics, College of Liberal Arts and Sciences, Iowa State 
      University, Ames, IA, USA.
FAU - Wang, Chong
AU  - Wang C
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA, USA; Department of 
      Statistics, College of Liberal Arts and Sciences, Iowa State University, Ames, 
      IA, USA.
FAU - Gimenez-Lirola, Luis
AU  - Gimenez-Lirola L
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA, USA.
FAU - Baum, David H
AU  - Baum DH
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA, USA.
FAU - Gauger, Phillip C
AU  - Gauger PC
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA, USA.
FAU - Harmon, Karen M
AU  - Harmon KM
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA, USA.
FAU - Hoogland, Marlin
AU  - Hoogland M
AD  - Smithfield Foods, Algona, IA, USA.
FAU - Main, Rodger
AU  - Main R
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA, USA.
FAU - Zimmerman, Jeffrey J
AU  - Zimmerman JJ
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA, USA.
LA  - eng
PT  - Journal Article
DEP - 20170217
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
SB  - IM
MH  - Animals
MH  - Diagnostic Techniques and Procedures/standards/veterinary
MH  - *Guidelines as Topic
MH  - *Housing, Animal
MH  - Models, Biological
MH  - Population Surveillance
MH  - Porcine Reproductive and Respiratory Syndrome/*diagnosis/prevention & 
      control/transmission
MH  - Porcine respiratory and reproductive syndrome virus/genetics
MH  - Research Design/*standards
MH  - Saliva/*virology
MH  - Swine
OTO - NOTNLM
OT  - Modeling
OT  - Monitoring
OT  - Oral fluid
OT  - PRRSV
OT  - Probability of detection
OT  - Sample size
OT  - Sampling
OT  - Surveillance
EDAT- 2017/03/13 06:00
MHDA- 2018/04/04 06:00
CRDT- 2017/03/13 06:00
PHST- 2016/10/18 00:00 [received]
PHST- 2017/02/05 00:00 [revised]
PHST- 2017/02/09 00:00 [accepted]
PHST- 2017/03/13 06:00 [pubmed]
PHST- 2018/04/04 06:00 [medline]
PHST- 2017/03/13 06:00 [entrez]
AID - S0378-1135(16)30527-2 [pii]
AID - 10.1016/j.vetmic.2017.02.004 [doi]
PST - ppublish
SO  - Vet Microbiol. 2017 Sep;209:20-29. doi: 10.1016/j.vetmic.2017.02.004. Epub 2017 
      Feb 17.