PMID- 28284435
OWN - NLM
STAT- MEDLINE
DCOM- 20170622
LR  - 20170622
IS  - 1355-0306 (Print)
IS  - 1355-0306 (Linking)
VI  - 57
IP  - 2
DP  - 2017 Mar
TI  - Identification of Cannabis sativa L. using the 1-kbTHCA synthase-fluorescence in 
      situ hybridization probe.
PG  - 101-106
LID - S1355-0306(16)30109-5 [pii]
LID - 10.1016/j.scijus.2016.11.002 [doi]
AB  - This study reports a successful application of fluorescence in situ hybridization 
      (FISH) technique in the identification of Cannabis sativa L. cells recovered from 
      fresh and dried powdered plant materials. Two biotin-16-dUTP-labeled FISH probes 
      were designed from the Cannabis-specific tetrahydrocannabinolic acid synthase 
      (THCAS) gene and the ITS region of the 45S rRNA gene. Specificity of probe-target 
      hybridization was tested against the target and 4 non-target plant species, i.e., 
      Humulus lupulus, Mitragyna speciosa, Papaver sp., and Nicotiana tabacum. The 1-kb 
      THCA synthase hybridization probe gave Cannabis-specific hybridization signals, 
      unlike the 700-bp Cannabis-ITS hybridization probe. Probe-target hybridization 
      was also confirmed against 20 individual Cannabis plant samples. The 1-kb THCA 
      synthase and 700-bp Cannabis-ITS hybridization probes clearly showed 2 
      hybridization signals per cell with reproducibility. The 1-kb THCA synthase probe 
      did not give any FISH signal when tested against H. lupulus, its closely related 
      member of the Canabaceae family. It was also showed that 1-kb THCA synthase FISH 
      probe can be applied to identify small amount of dried powdered Cannabis material 
      with an addition of rehydration step prior to the experimental process. This 
      study provided an alternative identification method for Cannabis trace.
CI  - Copyright (c) 2016. Published by Elsevier B.V.
FAU - Jeangkhwoa, Pattraporn
AU  - Jeangkhwoa P
AD  - M.Sc. Program in Plant Science, Department of Plant Science, Faculty of Science 
      and Department of Pharmaceutical Botany, Faculty of Pharmacy, Mahidol University, 
      Thailand.
FAU - Bandhaya, Achirapa
AU  - Bandhaya A
AD  - Forensic Science Program, Faculty of Science, Mahidol University, Thailand.
FAU - Umpunjun, Puangpaka
AU  - Umpunjun P
AD  - Department of Plant Science, Faculty of Science, Mahidol University, Thailand.
FAU - Chuenboonngarm, Ngarmnij
AU  - Chuenboonngarm N
AD  - Department of Plant Science, Faculty of Science, Mahidol University, Thailand.
FAU - Panvisavas, Nathinee
AU  - Panvisavas N
AD  - Forensic Science Program, Faculty of Science, Mahidol University, Thailand; 
      Department of Plant Science, Faculty of Science, Mahidol University, Thailand. 
      Electronic address: nathinee.pan@mahidol.ac.th.
LA  - eng
PT  - Journal Article
DEP - 20161105
PL  - England
TA  - Sci Justice
JT  - Science & justice : journal of the Forensic Science Society
JID - 9508563
RN  - 0 (RNA, Ribosomal)
RN  - 0 (RNA, ribosomal, 45S)
RN  - EC 5.3.- (Intramolecular Oxidoreductases)
RN  - EC 5.3.- (cannabidiolic acid synthase)
SB  - IM
MH  - Cannabis/*genetics
MH  - Forensic Toxicology
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Intramolecular Oxidoreductases/*genetics
MH  - RNA, Ribosomal/*genetics
OTO - NOTNLM
OT  - Cannabis sativa
OT  - Fluorescence in situ hybridization (FISH)
OT  - THCA synthase gene
EDAT- 2017/03/13 06:00
MHDA- 2017/06/24 06:00
CRDT- 2017/03/13 06:00
PHST- 2016/06/16 00:00 [received]
PHST- 2016/11/02 00:00 [revised]
PHST- 2016/11/04 00:00 [accepted]
PHST- 2017/03/13 06:00 [entrez]
PHST- 2017/03/13 06:00 [pubmed]
PHST- 2017/06/24 06:00 [medline]
AID - S1355-0306(16)30109-5 [pii]
AID - 10.1016/j.scijus.2016.11.002 [doi]
PST - ppublish
SO  - Sci Justice. 2017 Mar;57(2):101-106. doi: 10.1016/j.scijus.2016.11.002. Epub 2016 
      Nov 5.