PMID- 28285568
OWN - NLM
STAT- MEDLINE
DCOM- 20180329
LR  - 20180409
IS  - 1933-7205 (Electronic)
IS  - 1933-7191 (Linking)
VI  - 24
IP  - 7
DP  - 2017 Jul
TI  - Cigarette Smoking Affects Uterine Receptivity Markers.
PG  - 989-995
LID - 10.1177/1933719117697129 [doi]
AB  - OBJECTIVE: Smoking negatively affects fertility and the rate of other endometrial 
      diseases. To determine the effect of smoking on endometrial physiology, we 
      evaluated 2 endometrial regulatory cytokines and receptivity markers, C-X-C motif 
      chemokine ligand 12 (CXCL12) and fibroblast growth factor 2 (FGF2), both in vitro 
      and in vivo. STUDY DESIGN: The human endometrial stromal cell line (HESC) and 
      primary human endometrial stromal cells were treated with cigarette smoking 
      extract (CSE) or with vehicle control. Twenty female mice were randomly assigned 
      to either cigarette smoke (CS) exposure for 8 weeks or to a nonsmoke (NS) group 
      that received room air. Immunohistochemical analysis of CXCL12 and FGF2 
      expression was performed in mouse uterine tissue. Human endometrial samples were 
      obtained from both nonsmokers and smokers. Real-time reverse 
      transcription-polymerase chain reaction was performed for all cell cultures and 
      human samples. RESULTS: Compared to controls, CXCL12 and FGF2 mRNA expression 
      were significantly decreased in CSE-exposed HESC and primary cells. In mice, 
      immunohistochemical analysis showed that both CXCL12 and FGF2 protein expression 
      was lower in the CS group compared to controls. Similarly, both CXCL12 and FGF2 
      expression were decreased in women who smoke compared to nonsmokers. CONCLUSION: 
      Decreased endometrial CXCL12 and FGF2 expression contribute to the impaired 
      endometrial receptivity in women who smoke. Smoking is also associated with 
      decreased rates of endometrial cancer and endometriosis; increased CXCL12 and 
      FGF2 are implicated in both conditions. The changes in the expression of 
      cytokines described here may explain the impact of smoking on all of these 
      diseases. Tobacco has direct effects on normal endometrium that impacts 
      endometrial health and disease.
FAU - Sahin Ersoy, Gulcin
AU  - Sahin Ersoy G
AD  - 1 Department of Obstetrics, Gynecology and Reproductive Sciences, Yale School of 
      Medicine, New Haven, CT, USA.
FAU - Zhou, Yuping
AU  - Zhou Y
AD  - 1 Department of Obstetrics, Gynecology and Reproductive Sciences, Yale School of 
      Medicine, New Haven, CT, USA.
FAU - Inan, Hamdi
AU  - Inan H
AD  - 2 Department of Obstetrics and Gynecology, Tepecik Education and Research 
      Hospital, Izmir, Turkey.
FAU - Taner, Cuneyt E
AU  - Taner CE
AD  - 2 Department of Obstetrics and Gynecology, Tepecik Education and Research 
      Hospital, Izmir, Turkey.
FAU - Cosar, Emine
AU  - Cosar E
AD  - 1 Department of Obstetrics, Gynecology and Reproductive Sciences, Yale School of 
      Medicine, New Haven, CT, USA.
FAU - Taylor, Hugh S
AU  - Taylor HS
AD  - 1 Department of Obstetrics, Gynecology and Reproductive Sciences, Yale School of 
      Medicine, New Haven, CT, USA.
LA  - eng
PT  - Journal Article
DEP - 20170313
PL  - United States
TA  - Reprod Sci
JT  - Reproductive sciences (Thousand Oaks, Calif.)
JID - 101291249
RN  - 0 (Chemokine CXCL12)
RN  - 0 (Smoke)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
SB  - IM
MH  - Adult
MH  - Animals
MH  - Cell Line
MH  - Chemokine CXCL12/*metabolism
MH  - Cigarette Smoking/*metabolism
MH  - Endometrium/cytology/*metabolism
MH  - Female
MH  - Fibroblast Growth Factor 2/*metabolism
MH  - Humans
MH  - Mice
MH  - *Smoke
MH  - Stromal Cells/cytology/*metabolism
MH  - Young Adult
OTO - NOTNLM
OT  - CXCL12
OT  - FGF2
OT  - endometrial receptivity
OT  - smoking
EDAT- 2017/03/14 06:00
MHDA- 2018/03/30 06:00
CRDT- 2017/03/14 06:00
PHST- 2017/03/14 06:00 [pubmed]
PHST- 2018/03/30 06:00 [medline]
PHST- 2017/03/14 06:00 [entrez]
AID - 10.1177/1933719117697129 [doi]
PST - ppublish
SO  - Reprod Sci. 2017 Jul;24(7):989-995. doi: 10.1177/1933719117697129. Epub 2017 Mar 
      13.