PMID- 28286103
OWN - NLM
STAT- MEDLINE
DCOM- 20170824
LR  - 20170824
IS  - 1872-7573 (Electronic)
IS  - 0378-8741 (Linking)
VI  - 202
DP  - 2017 Apr 18
TI  - The vascular protective effects of Anoectochilus roxburghii polysaccharose under 
      high glucose conditions.
PG  - 192-199
LID - S0378-8741(16)30852-2 [pii]
LID - 10.1016/j.jep.2017.03.012 [doi]
AB  - ETHNOPHARMACOLOGICAL RELEVANCE: Anoectochilus roxburghii has been used as a 
      health food and a herb for treatment diabetes in China for hundreds years. 
      Anoectochilus roxburghii polysaccharose (ARP) is the major active component of 
      the plant. AIM OF THE STUDY: The present study investigated the vascular 
      protection of ARP in vivo and in vitro experiments. MATERIALS AND METHODS: 
      Hypoglycemic activity of ARP was examined in diabetic mice. Moreover, the further 
      vascular protective effects in vitro were investigated in human umbilical vein 
      endothelial cells (HUVECs) stimulated by high glucose (HG, 35mM). RESULTS: 
      Compared with untreated diabetic mice, ARP (100 or 300mg/kg) caused a significant 
      decrease in blood glucose levels. Histological examination showed that ARP 
      ameliorated endothelial damage to some extent, especially ARP at dosage of 
      300mg/kg. In vitro assay, pretreatment with ARP (10, 20 and 30mug/mL) markedly 
      inhibited generations of reactive oxygen species (ROS), monocyte chemoattractant 
      protein-1 (MCP-1) and intercellular adhesion molecule-1 (ICAM-1) in HG-induced 
      HUVECs. ARP pretreatment not only suppressed HG-induced matrix metalloproteinases 
      (MMPs) activity via increasing the expression of the tissue inhibitors of MMPs 
      (TIMPs), but also adjusted the MMPs/TIMPs balance to maintain homeostasis of 
      vascular structure. Moreover, pretreatment with ARP could significantly reduce 
      p-NF-kappaB p65, p-p38 MAPK expression levels in HG-induced HUVECs. CONCLUSIONS: The 
      vascular protective effects of ARP might be associated with NF-kappaB and p38 MAPK 
      pathway. ARP might be used as useful substance in the treatment of vasculopathy 
      in diabetic patients.
CI  - Copyright (c) 2017 Elsevier Ireland Ltd. All rights reserved.
FAU - Liu, Zhen-Ling
AU  - Liu ZL
AD  - State Key Laboratory of Applied Organic Chemistry, College of Chemistry and 
      Chemical Engineering, Lanzhou University, Lanzhou 730000, PR China. Electronic 
      address: liuzhl@lzu.edu.cn.
FAU - Zhang, Jian-Gang
AU  - Zhang JG
AD  - Institute of pathology, Lanzhou university, Lanzhou 730000, PR China.
FAU - Liu, Qing
AU  - Liu Q
AD  - Department of Chemical and Pharmaceutical Engineering, College of Chemical 
      Engineering, Huaqiao University, Xiamen 361021, Fujian province, PR China.
FAU - Yi, Li-Tao
AU  - Yi LT
AD  - Department of Chemical and Pharmaceutical Engineering, College of Chemical 
      Engineering, Huaqiao University, Xiamen 361021, Fujian province, PR China.
FAU - Li, Yu-Meng
AU  - Li YM
AD  - Department of Chemical and Pharmaceutical Engineering, College of Chemical 
      Engineering, Huaqiao University, Xiamen 361021, Fujian province, PR China.
FAU - Li, Ya
AU  - Li Y
AD  - State Key Laboratory of Applied Organic Chemistry, College of Chemistry and 
      Chemical Engineering, Lanzhou University, Lanzhou 730000, PR China.
LA  - eng
PT  - Journal Article
DEP - 20170310
PL  - Ireland
TA  - J Ethnopharmacol
JT  - Journal of ethnopharmacology
JID - 7903310
RN  - 0 (Antioxidants)
RN  - 0 (Blood Glucose)
RN  - 0 (Cytokines)
RN  - 0 (Hypoglycemic Agents)
RN  - 0 (Polysaccharides)
SB  - IM
MH  - Animals
MH  - Antioxidants/pharmacology
MH  - Aorta, Thoracic/drug effects
MH  - Blood Glucose/metabolism
MH  - Cytokines/biosynthesis
MH  - Diabetes Mellitus, Experimental/drug therapy
MH  - Diabetic Angiopathies/pathology/*prevention & control
MH  - Human Umbilical Vein Endothelial Cells
MH  - Humans
MH  - Hypoglycemic Agents/*pharmacology
MH  - Male
MH  - Medicine, Chinese Traditional
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Orchidaceae/*chemistry
MH  - Polysaccharides/chemistry/*pharmacology
MH  - Signal Transduction/drug effects
OTO - NOTNLM
OT  - Anoectochilus roxburghii
OT  - NF-kappaB
OT  - P38 MAPK
OT  - Polysaccharose
OT  - Vascular protection
EDAT- 2017/03/14 06:00
MHDA- 2017/08/25 06:00
CRDT- 2017/03/14 06:00
PHST- 2016/09/15 00:00 [received]
PHST- 2017/01/21 00:00 [revised]
PHST- 2017/03/09 00:00 [accepted]
PHST- 2017/03/14 06:00 [pubmed]
PHST- 2017/08/25 06:00 [medline]
PHST- 2017/03/14 06:00 [entrez]
AID - S0378-8741(16)30852-2 [pii]
AID - 10.1016/j.jep.2017.03.012 [doi]
PST - ppublish
SO  - J Ethnopharmacol. 2017 Apr 18;202:192-199. doi: 10.1016/j.jep.2017.03.012. Epub 
      2017 Mar 10.