PMID- 28286232
OWN - NLM
STAT- MEDLINE
DCOM- 20171121
LR  - 20211015
IS  - 1872-8057 (Electronic)
IS  - 0303-7207 (Linking)
VI  - 448
DP  - 2017 Jun 15
TI  - Steroid receptor coactivator-1 can regulate osteoblastogenesis independently of 
      estrogen.
PG  - 21-27
LID - S0303-7207(17)30173-9 [pii]
LID - 10.1016/j.mce.2017.03.005 [doi]
AB  - Steroid receptor coactivator-1 (SRC-1), a well-studied coactivator of estrogen 
      receptor (ER), is known to play an important and functional role in the 
      development and maintenance of bone tissue. Previous reports suggest SRC-1 
      maintains bone mineral density primarily through its interaction with ER. Here we 
      demonstrate that SRC-1 can also affect bone development independent of estrogen 
      signaling as ovariectomized SRC-1 knockout (SRC-1 KO) mouse had decreased bone 
      mineral density. To identify estrogen-independent SRC-1 target genes in 
      osteoblastogenesis, we undertook an integrated analysis utilizing ChIP-Seq and 
      mRNA microarray in transformed osteoblast-like U2OS-ERalpha cells. We identified 
      critical osteoblast differentiation genes regulated by SRC-1, but not by estrogen 
      including alkaline phosphatase and osteocalcin. Ex vivo primary culture of 
      osteoblasts from SRC-1 wild-type and KO mice confirmed the role of SRC-1 in 
      osteoblastogenesis, associated with altered ALPL levels. Together, these data 
      indicate that SRC-1 can impact osteoblast function in an ER-independent manner.
CI  - Copyright (c) 2017 Elsevier B.V. All rights reserved.
FAU - Watters, R J
AU  - Watters RJ
AD  - Women's Cancer Research Center, University of Pittsburgh Cancer Institute, Magee 
      Womens Research Institute, Pittsburgh, PA, USA; Department of Pharmacology & 
      Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, 
      USA; Department of Orthopaedic Surgery, University of Pittsburgh School of 
      Medicine, Pittsburgh, PA, USA. Electronic address: rjw63@pitt.edu.
FAU - Hartmaier, R J
AU  - Hartmaier RJ
AD  - Women's Cancer Research Center, University of Pittsburgh Cancer Institute, Magee 
      Womens Research Institute, Pittsburgh, PA, USA; Department of Pharmacology & 
      Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, 
      USA.
FAU - Osmanbeyoglu, H U
AU  - Osmanbeyoglu HU
AD  - Computational Biology Program, Memorial Sloan Kettering Cancer Center, New York, 
      USA.
FAU - Gillihan, R M
AU  - Gillihan RM
AD  - Department of Dermatology, University of Florida, Gainesville, FL, USA.
FAU - Rae, J M
AU  - Rae JM
AD  - Department of Pharmacology, University of Michigan, Ann Arbor, MI, USA.
FAU - Liao, L
AU  - Liao L
AD  - Department of Molecular & Cellular Biology, Baylor College of Medicine, Houston, 
      TX, USA.
FAU - Chen, K
AU  - Chen K
AD  - Institute for Academic Medicine & Department of Cardiovascular Sciences, The 
      Methodist Hospital Research Institute, Houston, TX 77030, USA.
FAU - Li, W
AU  - Li W
AD  - Dan L. Duncan Cancer Center, Baylor College of Medicine, Houston, TX, USA.
FAU - Lu, X
AU  - Lu X
AD  - Department of Biomedical Informatics, University of Pittsburgh School of 
      Medicine, Pittsburgh, PA, USA.
FAU - Oesterreich, S
AU  - Oesterreich S
AD  - Women's Cancer Research Center, University of Pittsburgh Cancer Institute, Magee 
      Womens Research Institute, Pittsburgh, PA, USA; Department of Pharmacology & 
      Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, 
      USA.
LA  - eng
GR  - K99 CA207871/CA/NCI NIH HHS/United States
GR  - R01 GM099143/GM/NIGMS NIH HHS/United States
GR  - R21 DK082825/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20170307
PL  - Ireland
TA  - Mol Cell Endocrinol
JT  - Molecular and cellular endocrinology
JID - 7500844
RN  - 0 (Estrogens)
RN  - EC 2.3.1.48 (Nuclear Receptor Coactivator 1)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/metabolism
MH  - Animals
MH  - Bone Density/drug effects
MH  - Cell Differentiation/drug effects
MH  - Cell Line, Tumor
MH  - Estrogens/*pharmacology
MH  - Humans
MH  - Mice, Knockout
MH  - Nuclear Receptor Coactivator 1/*metabolism
MH  - Osteoblasts/cytology/drug effects/*metabolism
MH  - *Osteogenesis
OTO - NOTNLM
OT  - Bone
OT  - Estrogen receptor
OT  - NCOA1
OT  - SRC-1
EDAT- 2017/03/14 06:00
MHDA- 2017/11/29 06:00
CRDT- 2017/03/14 06:00
PHST- 2016/09/02 00:00 [received]
PHST- 2017/03/04 00:00 [revised]
PHST- 2017/03/04 00:00 [accepted]
PHST- 2017/03/14 06:00 [pubmed]
PHST- 2017/11/29 06:00 [medline]
PHST- 2017/03/14 06:00 [entrez]
AID - S0303-7207(17)30173-9 [pii]
AID - 10.1016/j.mce.2017.03.005 [doi]
PST - ppublish
SO  - Mol Cell Endocrinol. 2017 Jun 15;448:21-27. doi: 10.1016/j.mce.2017.03.005. Epub 
      2017 Mar 7.