PMID- 28287715
OWN - NLM
STAT- MEDLINE
DCOM- 20190301
LR  - 20190301
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 89
IP  - 7
DP  - 2017 Apr 4
TI  - Dual-Recognition Forster Resonance Energy Transfer Based Platform for One-Step 
      Sensitive Detection of Pathogenic Bacteria Using Fluorescent Vancomycin-Gold 
      Nanoclusters and Aptamer-Gold Nanoparticles.
PG  - 4085-4090
LID - 10.1021/acs.analchem.6b04958 [doi]
AB  - The effective monitoring, identification, and quantification of pathogenic 
      bacteria is essential for addressing serious public health issues. In this study, 
      we present a universal and facile one-step strategy for sensitive and selective 
      detection of pathogenic bacteria using a dual-molecular affinity-based Forster 
      (fluorescence) resonance energy transfer (FRET) platform based on the recognition 
      of bacterial cell walls by antibiotic and aptamer molecules, respectively. As a 
      proof of concept, Vancomycin (Van) and a nucleic acid aptamer were employed in a 
      model dual-recognition scheme for detecting Staphylococcus aureus (Staph. 
      aureus). Within 30 min, by using Van-functionalized gold nanoclusters and 
      aptamer-modified gold nanoparticles as the energy donor and acceptor, 
      respectively, the FRET signal shows a linear variation with the concentration of 
      Staph. aureus in the range from 20 to 10(8) cfu/mL with a detection limit of 10 
      cfu/mL. Other nontarget bacteria showed negative results, demonstrating the good 
      specificity of the approach. When employed to assay Staph. aureus in real 
      samples, the dual-recognition FRET strategy showed recoveries from 99.00% to the 
      109.75% with relative standard derivations (RSDs) less than 4%. This establishes 
      a universal detection platform for sensitive, specific, and simple pathogenic 
      bacteria detection, which could have great impact in the fields of food/public 
      safety monitoring and infectious disease diagnosis.
FAU - Yu, Mengqun
AU  - Yu M
AD  - Key Laboratory of Luminescence and Real-Time Analytical Chemistry, Ministry of 
      Education, College of Pharmaceutical Sciences, Southwest University , Chongqing, 
      400715, People's Republic of China.
FAU - Wang, Hong
AU  - Wang H
AD  - Key Laboratory of Luminescence and Real-Time Analytical Chemistry, Ministry of 
      Education, College of Pharmaceutical Sciences, Southwest University , Chongqing, 
      400715, People's Republic of China.
FAU - Fu, Fei
AU  - Fu F
AD  - Key Laboratory of Luminescence and Real-Time Analytical Chemistry, Ministry of 
      Education, College of Pharmaceutical Sciences, Southwest University , Chongqing, 
      400715, People's Republic of China.
FAU - Li, Linyao
AU  - Li L
AD  - Key Laboratory of Luminescence and Real-Time Analytical Chemistry, Ministry of 
      Education, College of Pharmaceutical Sciences, Southwest University , Chongqing, 
      400715, People's Republic of China.
FAU - Li, Jing
AU  - Li J
AD  - Key Laboratory of Luminescence and Real-Time Analytical Chemistry, Ministry of 
      Education, College of Pharmaceutical Sciences, Southwest University , Chongqing, 
      400715, People's Republic of China.
FAU - Li, Gan
AU  - Li G
AD  - Key Laboratory of Luminescence and Real-Time Analytical Chemistry, Ministry of 
      Education, College of Pharmaceutical Sciences, Southwest University , Chongqing, 
      400715, People's Republic of China.
FAU - Song, Yang
AU  - Song Y
AUID- ORCID: 0000-0001-7716-9216
AD  - Key Laboratory of Luminescence and Real-Time Analytical Chemistry, Ministry of 
      Education, College of Pharmaceutical Sciences, Southwest University , Chongqing, 
      400715, People's Republic of China.
FAU - Swihart, Mark T
AU  - Swihart MT
AUID- ORCID: 0000-0002-9652-687X
AD  - Department of Chemical and Biological Engineering, University at Buffalo, State 
      University of New York , Buffalo, New York 14260, United States.
FAU - Song, Erqun
AU  - Song E
AUID- ORCID: 0000-0003-4026-090X
AD  - Key Laboratory of Luminescence and Real-Time Analytical Chemistry, Ministry of 
      Education, College of Pharmaceutical Sciences, Southwest University , Chongqing, 
      400715, People's Republic of China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20170327
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Aptamers, Nucleotide)
RN  - 6Q205EH1VU (Vancomycin)
RN  - 7440-57-5 (Gold)
SB  - IM
MH  - Aptamers, Nucleotide/*chemistry
MH  - Fluorescence
MH  - *Fluorescence Resonance Energy Transfer
MH  - Gold/*chemistry
MH  - Nanoparticles/*chemistry
MH  - Particle Size
MH  - Staphylococcus aureus/*isolation & purification
MH  - Surface Properties
MH  - Vancomycin/*chemistry
EDAT- 2017/03/14 06:00
MHDA- 2019/03/02 06:00
CRDT- 2017/03/14 06:00
PHST- 2017/03/14 06:00 [pubmed]
PHST- 2019/03/02 06:00 [medline]
PHST- 2017/03/14 06:00 [entrez]
AID - 10.1021/acs.analchem.6b04958 [doi]
PST - ppublish
SO  - Anal Chem. 2017 Apr 4;89(7):4085-4090. doi: 10.1021/acs.analchem.6b04958. Epub 
      2017 Mar 27.